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001-es BibID:	BIBFORM035095
Első szerző:	Balkay László (biofizikus)
Cím:	A novel method for measuring intracellular pH : effect of neutron irradiation on pHi of transformed cells / László Balkay, Teréz Márián, Miklós Emri, Lajos Trón
Dátum:	1992
ISSN:	1011-1344
Megjegyzések:	A new flow cytometric method was developed for measuring the intracellular pH (pHi) of mammalian cells using a fluorescent pH indicator dye 2',7-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF). Emission intensities (or their ratios) measured from BCECF-loaded cells can be converted into absolute pHi values using appropriate calibration curves. By comparison of several possible measuring and data evaluation procedures a double-ratio method was suggested as the most advantageous protocol to yield reliable intracellular pH data. This method allows pHi to be determined on a cell-by-cell basis corrected for cell volume and change in geometry of input-output optics of the flow cytometer. Our method applies a standard calibration curve and does not necessitate its reconstruction for each new set of measurements. Cells of the OKT-4 and OKT-8 hybridoma lines were exposed to neutron irradiation of different doses. Irradiated cells underwent a biphasic alkalinization; an instantaneous effect detected within 1.5 h was found to be intensified over 24 h. For the interpretation of data we suggest that the increase in cytoplasmic pH following neutron treatment is evoked by two mechanisms.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal of Photochemistry and Photobiology B-Biology. - 16 : 3-4 (1992), p. 367-375. -
További szerzők:	Márián Teréz (1950-) (radiobiológus) Emri Miklós (1962-) (fizikus) Trón Lajos (1941-) (biofizikus)
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001-es BibID:	BIBFORM046276
Első szerző:	Krasznai Zoltán (biofizikus)
Cím:	Flow cytometric determination of absolute membrane potential of cells / Krasznai Z., Marian T., Balkay L., Emri M., Tron L.
Dátum:	1995
Megjegyzések:	Membrane potential measurements using fluorescent membrane potential indicator dyes report on relative changes but usually do not result in an absolute value of the measured parameter. The method developed in this paper is based on the assumption that the negatively charged bis-oxonol distributes across the cytoplasmic membrane according to the Nernst equation. It is further supposed that the fluorescence intensity measured from a given stained cell is a single-value function of the intracellular dye concentration. The protocol suggested incorporates the construction of a calibration curve (fluorescence intensity measured from stained cells vs. extracellular dye concentration). This allows the evaluation of the membrane potential in millivolts using fluorescence readings of the cells both in the depolarized state and in the state of interest. Good agreement was found between absolute membrane potential data of human peripheral blood lymphocytes by our method and results of parallel patch clamp measurements.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal of Photochemistry and Photobiology. B, Biology. - 28 : 1 (1995), p. 93-99. -
További szerzők:	Márián Teréz (1950-) (radiobiológus) Balkay László (1963-) (biofizikus) Emri Miklós (1962-) (fizikus) Trón Lajos (1941-) (biofizikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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