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001-es BibID:	BIBFORM042927
035-os BibID:	PMID:23466426
Első szerző:	Palicz Zoltán (orvos)
Cím:	In vivo application of a small molecular weight antifungal protein of Penicillium chrysogenum (PAF) / Zoltán Palicz, Ágnes Jenes, Tamás Gáll, Kornél Miszti-Blasius, Sándor Kollár, Ilona Kovács, Miklós Emri, Teréz Márián, Éva Leiter, István Pócsi, Éva Csősz, Gergő Kalló, Csaba Hegedűs, László Virág, László Csernoch, Péter Szentesi
Dátum:	2013
ISSN:	0041-008X
Megjegyzések:	The antifungal protein of Penicillium chrysogenum (PAF) inhibits the growth of important pathogenic filamentous fungi, including members of the Aspergillus family and some dermatophytes. Furthermore, PAF was proven to have no toxic effects on mammalian cells in vitro. To prove that PAF could be safely used in therapy, experiments were carried out to investigate its in vivo effects. Adult mice were inoculated with PAF intranasally in different concentrations, up to 2700g·kg(-1) daily, for 2weeks. Even at the highest concentration - a concentration highly toxic in vitro for all affected molds - used, animals neither died due to the treatment nor were any side effects observed. Histological examinations did not find pathological reactions in the liver, in the kidney, and in the lungs. Mass spectrometry confirmed that a measurable amount of PAF was accumulated in the lungs after the treatment. Lung tissue extracts from PAF treated mice exerted significant antifungal activity. Small-animal positron emission tomography revealed that neither the application of physiological saline nor that of PAF induced any inflammation while the positive control lipopolysaccharide did. The effect of the drug on the skin was examined in an irritative dermatitis model where the change in the thickness of the ears following PAF application was found to be the same as in control and significantly less than when treated with phorbol-12-myristate-13-acetate used as positive control. Since no toxic effects of PAF were found in intranasal application, our result is the first step for introducing PAF as potential antifungal drug in therapy.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Molekuláris Medicina
Megjelenés:	Toxicology and Applied Pharmacology 269 : 1 (2013), p. 8-16. -
További szerzők:	Jenes Ágnes (1980-) (élettanász) Gáll Tamás (1982-) (molekuláris biológus, mikrobiológus) Miszti-Blasius Kornél (1977-) (laboratóriumi szakorvos) Kollár Sándor (1949-) (sebész) Kovács Ilona (1965-) (patológus) Emri Miklós (1962-) (fizikus) Márián Teréz (1950-) (radiobiológus) Leiter Éva (1976-) (biológus) Pócsi István (1961-) (vegyész) Csősz Éva (1977-) (biokémikus, molekuláris biológus) Kalló Gergő (1989-) (molekuláris biológus) Hegedűs Csaba (1980-) (biokémikus, molekuláris biológus) Virág László (1965-) (biokémikus, sejtbiológus, farmakológus) Csernoch László (1961-) (élettanász) Szentesi Péter (1967-) (élettanász)
Pályázati támogatás:	TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Oxidatív stressz és ADP-riboziláció kapcsolatának vizsgálata TÁMOP-4.2.2/B-10/1-2010-0024 TÁMOP Molekuláris Orvostudomány Doktori Iskola TÁMOP-4.2.2.A-11/1/KONV-2012-0025 TÁMOP
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001-es BibID:	BIBFORM015778
Első szerző:	Trencsényi György (biológus, biokémikus, molekuláris biológus)
Cím:	Renal capsule parathymic lymph node complex : imaging of a new in vivo metastatic model in rats with 18F-FDG / Gy. Trencsenyi, P. Kertai , F. Bako, J. Hunyadi, T. Marian, S. A. Kis, G. Opposits, M. Emri, I. Pocsi, G. Banfalvi
Dátum:	2009
Megjegyzések:	Background: In our experiments, we wished to prove - using mini-PET, autora- diography and organ distribution examinations - that rat hepatocarcinoma (He/De), and mesoblastic nephroma (Ne/De) cells give metastases to the parathy- mic lymph nodes. Material and methods: 106 He/De or Ne/De cells were placed under the capsule of the left kidney. Two weeks after implantation, control and tumor-bearing rats were anesthetized and a radioligand, 18FDG (15.0 MBq), was injected i.v. For autorad- iography, 60 ?m thick cryostate sections (Leica cryomacrotome) were cut and ex- posed to phosphor imaging plates. Results were expressed in intensity pixel units. For organ-distribution, different tissues were removed and their activities were mea- sured. The radioactivity of the samples was used to determine the differential ab- sorption ratio (DAR). Results: By taking the pixel density of resting striated muscle as one unit, the rela- tive pixel densities were in decreasing order: 14.23 in He/De tumor, 10.82 in par- athymic lymph nodes, 5.36 in kidney, 2.35 in blood and 1.57 in liver. In the case of the Ne/De, the results were the same. The DAR values also showed that the majority of the radioactivity was accumulated in the tumors and in the parathymic lymph nodes. Conclusions: From the autoradiographic, phosphor image and tissue distribution experiments, we concluded that He/De and Ne/De tumors grown under the cap- sule of kidney represent a significant metastatic burden manifested primarily in parathymic lymph nodes. The renal capsule and parathymic lymph node complex seems to be suitable for the isolated in vivo examination of metastatic development and for the detailed analysis of secondary tumors.
Tárgyszavak:	Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:	Nuclear Medicine Review 12 : 1 (2009), p. 39. -
További szerzők:	Kertai Pál (1927-2016) (népegészségügyi szakember) Bakó Ferenc (biológus) Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Márián Teréz (1950-) (radiobiológus) Kis Sándor Attila (1973-) (fizikus) Opposits Gábor (1974-) (fizikus, szoftver fejlesztő) Emri Miklós (1962-) (fizikus) Pócsi István (1961-) (vegyész) Bánfalvi Gáspár (1943-) (sejtbiológus, gyógyszerész)
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