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001-es BibID:BIBFORM020318
Első szerző:Jakab Balázs (Pécs)
Cím:Distribution of PACAP-38 in the central nervous system of various species determined by a novel radioimmunoassay / Balázs Jakab, Dóra Reglődi, Rita Józsa, Tibor Hollósy, Andrea Tamás, Andrea Lubics, István Lengvári, Gábor Oroszi, Zoltán Szilvássy, János Szolcsányi, József Németh
Dátum:2004
Megjegyzések:AbstractPituitary adenylate cyclase activating polypeptide (PACAP) occurs in two molecular forms: PACAP-38 and PACAP-27. Soon after the isolation and chemical characterization of PACAP, the first radioimmunoassay (RIA) methods have been developed, but it is a still rarely used laboratory technique in the field of PACAP research. The aim of the present study was to develop a novel, highly specific PACAP-38 assay to investigate the quantitative distribution of PACAP-38 in the central nervous system of various vertebrate species under the same technical and experimental conditions. Different areas of the brain and the spinal cord were removed from rats, chickens and fishes and the tissue samples were processed for PACAP-38 RIA. Our results indicate that the antiserum used in the RIA is C-terminal specific, without affinity for other members of the vasoactive intestinal polypeptide (VIP)/secretin/glucagon peptide family. The average ID50 value was 48.6+/-3.4 fmol/ml determined in 10 consecutive assays. Detection limit for PACAP-38 proved to be 2 fmol/ml. PACAP-38 immunoreactivity was present in the examined brain areas of each species studied, with highest concentration in the rat diencephalons. High levels of PACAP-38 were also detected in the rat telencephalon, followed by spinal cord and brainstem. The central nervous system of the fish also contained considerable concentrations of PACAP-38, whereas lowest concentrations were measured in the central nervous system of the chicken.
Tárgyszavak:Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
PACAP-38
central nervous system
radioimmunoassay
Megjelenés:Journal of Biochemical and Biophysical Methods. - 61 : 1-2 (2004), p. 189-198. -
További szerzők:Reglődi Dóra (Idegtudományok) Józsa Rita (Pécs) Hollósy Tibor (Pécs) Tamás Andrea (Idegtudomány) (Pécs) Lubics Andrea (Pécs) Lengvári István Oroszi Gábor (Pécs) Szolcsányi János (Pécs) Németh József (Pécs) Szilvássy Zoltán (1957-) (belgyógyász, farmakológus, klinikai farmakológus)
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2.

001-es BibID:BIBFORM002473
Első szerző:Kiss P.
Cím:Changes of PACAP levels in the brain show gender differences following short-term water and food deprivation / P. Kiss, D. Reglödi, A. Tamás, A. Lubics, I. Lengvári, R. Józsa, A. Somogyvári-Vigh, Z. Szilvássy, J. Németh
Dátum:2007
Megjegyzések:Pituitary adenylate cyclase activating polypeptide (PACAP) is a pleiotropic neuropeptide exerting diverse actions in the central and peripheral nervous systems. A few studies indicate that PACAP is involved in the regulation of feeding and water homeostasis. The aim of the present study was to investigate changes in PACAP38 concentrations in diVerent brain areas following food or water deprivation in male and female rats. Rats were sacriWced 12, 36 and 84 h after water or food removal. PACAP levels were determined by radioimmunoassay. Our results show that levels of PACAP decreased in the hypothalamus in both sexes after water deprivation, with a more marked, signiWcant decrease in females at 12 h. A decrease was observed also in the telencephalon, with a similar pattern in both genders: levels were lowest after 12 h, and showed a gradual increase at the other two time-points. PACAP levels increased in the brainstem of male rats, while females had a decrease 12 h after water deprivation. The pattern of changes in PACAP levels was very diVerent after food deprivation. In male rats, PACAP levels showed a signiWcant increase in the hypothalamus, telencephalon and brainstem 12 h after the beginning of starvation. In females, a less marked increase was observed only in the hypothalamus while no changes were found in the other brain areas. Our results show a sensitive reaction in changes of endogenous PACAP levels to water and food deprivation in most brain areas, but they are diVerentially regulated in male and female rats.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Gender differences
RIA
Starvation
CNS
Megjelenés:General and Comparative Endocrinology. - 152 : 2-3 (2007), p. 225-230. -
További szerzők:Józsa Rita (Pécs) Szilvássy Zoltán (1957-) (belgyógyász, farmakológus, klinikai farmakológus) Németh József (1954-) (vegyész, analitikus) Reglődi Dóra (Idegtudományok) Tamás Andrea (Idegtudomány) (Pécs) Lubics Andrea (Pécs) Lengvári István Somogyvári-Vigh Anikó
Internet cím:elektronikus változat
DOI
Szerző által megadott URL
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3.

001-es BibID:BIBFORM003049
Első szerző:Németh József (vegyész, analitikus)
Cím:PACAP-27 radioimmunoassay : description and application of a novel method / J. Nemeth, B. Jakab, R. Jozsa, T. Hollossy, A. Tamas, A. Lubics, I. Lengvari, P. Kiss, Zs. Oberritter, B. Horvath, Z. Szilvassy, D. Reglődi
Dátum:2007
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény hazai lapban
PACAP-27 radioimmunoassay
Megjelenés:Journal of Radioanalytical and Nuclear Chemistry. - 273 : 2 (2007), p. 327-332. -
További szerzők:Jakab Balázs (Pécs) Józsa Rita (Pécs) Hollósy Tibor (Pécs) Tamás Andrea (Idegtudomány) (Pécs) Lubics Andrea (Pécs) Lengvári István Kiss P. Oberritter Zsolt Horváth B. Reglődi Dóra (Idegtudományok) Szilvássy Zoltán (1957-) (belgyógyász, farmakológus, klinikai farmakológus)
Internet cím:elektronikus változat
DOI
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