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1.

001-es BibID:BIBFORM015989
Első szerző:Gáspár Rezső (biofizikus)
Cím:Measurement and analysis of different aspects of potassium currents in human lymphocytes / R. Gáspár, Z. Varga, Gy. Panyi, Z. Krasznai, C. Pieri, S. Damjanovich
Dátum:1998
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
Megjelenés:Signal Transduction. Single cell techniques / eds. Bert van Duijn; Anneke Wiltink. - p. 214-235.
További szerzők:Varga Zoltán (1969-) (biofizikus, szakfordító) Panyi György (1966-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) Pieri, Carlo Damjanovich Sándor (1936-2017) (biofizikus)
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2.

001-es BibID:BIBFORM006036
Első szerző:Gáspár Rezső (biofizikus)
Cím:Effects of bretylium tosylate on voltage-gated potassium channels in human T lymphocytes / Gaspar, R., Panyi, G., Ypey, D. L., Krasznai, Z., Vereb, G., Pieri, C., Damjanovich, S.
Dátum:1994
Megjegyzések:Using the patch-clamp technique, we determined that bretylium tosylate, a quaternary ammonium compound possessing immunomodulating activity, decreased the whole-cell K+ current in human T lymphocytes, in a dose-dependent manner, in the 0.05-5 mM extracellular concentration range. Bretylium tosylate prolonged the recovery from inactivation and accelerated the inactivation and deactivation of the K+ current but did not influence the kinetics of activation or the voltage dependence of activation and steady state inactivation of the K+ conductance. The percentage of drug-induced block was independent of membrane potential. K+ channel block by bretylium tosylate was partially and slowly removable by washing with drug-free extracellular solution. Bovine serum albumin (10 mg/ml) in the bath lifted the drug-induced block almost instantaneously, although not completely. In control experiments bovine serum albumin increased the inactivation time constant of the K+ channels but left the peak K+ current amplitude unaffected. On the basis of the experimental evidence, a gating-dependent allosteric interaction is suggested for the mechanism of drug action. The effective dose range, time of exposure, and reversibility of bretylium tosylate-induced K+ channel block correlated well with the same parameters of the drug-induced inhibition of T lymphocyte activation. The reported effects of bretylium tosylate on T cell mitogenesis can be regarded partly as a consequence of its blocking effects on voltage-gated K+ channels.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Bretylium Tosylate
Cell Membrane
drug effects
Electrophysiology
Human
Hungary
In Vitro
Ion Channel Gating
Kinetics
Lymphocytes
pharmacology
physiology
Potassium
Potassium Channels
Support,Non-U.S.Gov't
T-Lymphocytes
Megjelenés:Molecular pharmacology. - 46 : 4 (1994), p. 762-766. -
További szerzők:Panyi György (1966-) (biofizikus) Ypey, Dirk L. Krasznai Zoltán (1950-) (biofizikus) Vereb György (1965-) (biofizikus, orvos) Pieri, Carlo Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
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3.

001-es BibID:BIBFORM004728
Első szerző:Hajdu Péter (biofizikus)
Cím:Cholesterol modifies the gating of Kv1.3 in human T lymphocytes / Péter Hajdú, Zoltán Varga, Carlo Pieri, György Panyi, Rezső Gáspár
Dátum:2003
ISSN:0031-6768
Megjegyzések:The Kv1.3 potassium channel that belongs to the Shaker family of voltage-gated K(+) channels plays a crucial role in the mitogenic response of T cells. Because it spans the cell membrane its function can be influenced by lipid-protein interactions. In order to study the effect of lipid-protein interactions on the functioning of Kv1.3 we manipulated the membrane cholesterol content in T cells mimicking various physiological conditions by means of the oligosaccharide methyl-beta-cyclodextrin (MbetaCD) and its cholesterol-saturated complex (MbetaCD/C). Fluorescence polarization anisotropy and peak current density were used to monitor the efficiency of cholesterol removal (MbetaCD) and loading (MbetaCD/C). Using whole-cell patch-clamp technique we determined the kinetic and steady-state parameters of activation and inactivation of the Kv1.3 currents under different treatment conditions. Upon elevation of cholesterol content by 1 or 1.5 mg/ml MbetaCD/C the rates of both activation and inactivation were slowed. Moreover, the increased cholesterol level in the membrane resulted in a biphasic activation curve. Cholesterol depletion with MbetaCD (0.95 and 1.425 mg/ml) caused no significant changes in the gating characteristics of Kv1.3. The equilibrium between the open and the closed states of the channels was affected by increased cholesterol content, but at the same time steady-state inactivation was unchanged. We argue that manipulation of membrane cholesterol changed both the kinetic properties of Kv1.3 and steady-state parameters of activation by modifying lipid-protein interactions
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
beta-Cyclodextrins
Biophysics
Cell Membrane
Cells
Cholesterol
Cyclodextrins
drug effects
Electric Conductivity
Fluorescence
Fluorescence Polarization
Human
Humans
Hungary
Ion Channel Gating
Kinetics
Kv1.3 Potassium Channel
Lymphocytes
Membrane Microdomains
Membrane Potentials
metabolism
pharmacology
physiology
Potassium
Potassium Channels
Potassium Channels,Voltage-Gated
Research
Support
T-Lymphocytes
Megjelenés:Pflügers Archiv. - 445 : 6 (2003), p. 674-682. -
További szerzők:Varga Zoltán (1969-) (biofizikus, szakfordító) Pieri, Carlo Panyi György (1966-) (biofizikus) Gáspár Rezső (1944-) (biofizikus)
Internet cím:DOI
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4.

001-es BibID:BIBFORM004715
Első szerző:Panyi György (biofizikus)
Cím:Pharmacological effects of melatonin on ion channels / Panyi, G., Somodi, S., Varga, Z., Hajdu, P., Pieri, C., Pandi-Perumal, S. R., Damjanovich, S., Gaspar, R., Hardar, C., Singaravel, M., Maitra, S. K.
Dátum:2002
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
Ion Channels
Melatonin
Megjelenés:Treatise on Pineal Gland and Melatonin. - p. 489-506.
További szerzők:Somodi Sándor (1977-) (belgyógyász) Varga Zoltán (1969-) (biofizikus, szakfordító) Hajdu Péter (1975-) (biofizikus) Pieri, Carlo Pandi-Perumal, Seithikurippu R. Damjanovich Sándor (1936-2017) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Hardar, C. Singaravel, M. Maitra, S. K.
Borító:

5.

001-es BibID:BIBFORM004635
Első szerző:Péter Mózes (orvos, neuroradiológus) ifj.
Cím:Pandinus imperator scorpion venom blocks voltage-gated K+ channels in human lymphocytes / Peter, M. Jr., Varga, Z., Panyi, G., Bene, L., Damjanovich, S., Pieri, C., Possani, L. D., Gaspar, R.
Dátum:1998
ISSN:006-291X
Megjegyzések:Using the patch-clamp technique, we determined that Pandinus imperator scorpion venom blocked whole-cell n-type K+ currents in human peripheral blood lymphocytes in a dose-dependent manner with Kd = 0.02 microgram/ml. K+ channel block was instantaneous and removable by washing with venom-free extracellular solution. The venom-induced block was independent of membrane potential. The venom did not influence activation and inactivation kinetics of the K+ channels, however, accelerated recovery from inactivation. Purified peptides Pi1, Pi2, and Pi3 from the P. imperator venom powerfully blocked Kv1.3 channels in human lymphocytes with Kd values of 9.7 nM, 50 pM, and 0.5 nM, respectively. Flow cytometric membrane potential measurements with the oxonol dye showed that Pi2, the most effective peptide toxin of the P. imperator venom, depolarizes human lymphocytes in accordance with its K+ channel blocking effect.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Barbiturates
blood
drug effects
Electrophysiology
Flow Cytometry
Fluorescent Dyes
Human
isolation and purification
Isoxazoles
Kinetics
Lymphocytes
Membrane Potentials
metabolism
Patch-Clamp Techniques
pharmacology
physiology
Potassium
Potassium Channel Blockers
Potassium Channels
Protein Binding
Scorpion Venoms
Support, Non-U.S.Gov't
Support, U.S.Gov't, P.H.S.
Toxins
Megjelenés:Biochemical and Biophysical Research Communications. - 242 : 3 (1998), p. 621-625. -
További szerzők:Varga Zoltán (1969-) (biofizikus, szakfordító) Panyi György (1966-) (biofizikus) Bene László (1963-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Pieri, Carlo Possani, Lourival Domingos Gáspár Rezső (1944-) (biofizikus)
Internet cím:DOI
elektronikus változat
Borító:

6.

001-es BibID:BIBFORM040092
Első szerző:Varga Zoltán (biofizikus, szakfordító)
Cím:Multiple Binding Sites for Melatonin on Kv1.3 / Varga, Z., Panyi, G., Peter, M., Jr., Pieri, C., Csecsei, G., Damjanovich, S., Gaspar, R.
Dátum:2001
ISSN:0006-3495
Megjegyzések:Melatonin is a small amino acid derivative hormone of the pineal gland. Melatonin quickly and reversibly blocked Kv1.3 channels, the predominant voltage-gated potassium channel in human T-lymphocytes, acting from the extracellular side. The block did not show state or voltage dependence and was associated with an increased inactivation rate of the current. A half-blocking concentration of 1.5 mM was obtained from the reduction of the peak current. We explored several models to describe the stoichiometry of melatonin-Kv1.3 interaction considering one or four independent binding sites per channel. The model in which the occupancy of one of four binding sites by melatonin is sufficient to block the channels gives the best fit to the dose-response relationship, although all four binding sites can be occupied by the drug. The dissociation constant for the individual binding sites is 8.11 mM. Parallel application of charybdotoxin and melatonin showed that both compounds can simultaneously bind to the channels, thereby localizing the melatonin binding site out of the pore region. However, binding of tetraethylammonium to its receptor decreases the melatonin affinity, and vice versa. Thus, the occupancy of the two separate receptor sites allosterically modulates each other.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biophysical Journal. - 80 : 3 (2001), p. 1280-1297. -
További szerzők:Panyi György (1966-) (biofizikus) Péter Mózes (1972-) (orvos, neuroradiológus) ifj. Pieri, Carlo Csécsei György (1948-2005) (idegsebész) Damjanovich Sándor (1936-2017) (biofizikus) Gáspár Rezső (1944-) (biofizikus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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7.

001-es BibID:BIBFORM033278
035-os BibID:PMID:8588941 WOS:A1995TF18900001
Első szerző:Vereb György (biofizikus, orvos)
Cím:Plasma-membrane-bound macromolecules are dynamically aggregated to form non-random codistribution patterns of selected functional elements. Do pattern recognition processes govern antigen presentation and intercellular interactions? / György Vereb, László Matyus, László Bene, György Panyi, Zsolt Bacsó, Margit Balázs, János Matkó, János Szöllősi, Rezső Gáspár, Sándor Damjanovich, Robert E. Dale, Carlo Pieri, Marcel Ameloot
Dátum:1995
Megjegyzések:Molecular recognition processes between cell surface elements are discussed with special reference to cell surface pattern formation of membrane-bound integral proteins. The existence, as detected by flow cytometric resonance energy transfer (Appendix), and significance of cell surface patterns involving the interleukin-2 receptor, the T-cell receptor-CD3 system, the intercellular adhesion molecule ICAM-1, and the major histocompatibility complex class I and class II molecules in the plasma membrane of lymphocytes are described. The modulation of antigen presentation by transmembrane potential changes is discussed, and a general role of transmembrane potential changes, and therefore of ion channel activities, adduced as one of the major regulatory mechanisms of cell-cell communication. A general role in the mediation and regulation of intercellular interactions is suggested for cell-surface macromolecular patterns. The dynamic pattern of protein and lipid molecules in the plasma membrane is generated by the genetic code, but has a remarkable flexibility and may be one of the major instruments of accommodation and recognition processes at the cellular level.
Tárgyszavak:Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban
cell surface
molecular pattern
energy transfer
fluorescence
flow cytometry
transmembrane potential
MHC
antigen presentation
intercellular communication
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of Molecular Recognition. - 8 : 4 (1995), p. 237-246. -
További szerzők:Mátyus László (1956-) (biofizikus) Bene László (1963-) (biofizikus) Panyi György (1966-) (biofizikus) Bacsó Zsolt (1963-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Matkó János (1952-) (biológus) Szöllősi János (1953-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Dale, Robert E. Pieri, Carlo Ameloot, Marcel
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
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