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001-es BibID:	BIBFORM033103
035-os BibID:	PMID:19597833
Első szerző:	Csomor Péter (biotechnológus)
Cím:	Restriction analysis of otosclerosis-associated CD46 splicing variants / Csomor Péter, Szalmás Anita, Kónya József, Sziklai István, Karosi Tamás
Dátum:	2010
ISSN:	0937-4477
Megjegyzések:	Otosclerosis is a primary bone remodeling disorder of the human otic capsule and is associated with persistent measles virus infection. The human cellular receptor of measles virus is the membrane cofactor protein (MCP, CD46), which has 14 well-described splicing variants. Unique CD46 expression pattern of the otic capsule and the stapes footplate may determine the susceptibility for persistent measles virus infection. A total of 51 surgically removed ankylotic stapes footplates were analyzed by histopathological and molecular biological methods, respectively. Nucleic acids were extracted. Measles virus sequences were detected by nucleoprotein RNA-specific reverse transcriptase polymerase chain reaction (RT-PCR). Alternatively spliced RNA of CD46 isoforms was amplified by RT-PCR; cDNA amplimers were separated by SDS poly-acrylamide gel electrophoresis and were purified from the gel. Complementary DNA of CD46 isoforms was restricted by endonuclease enzymes having CD46-specific recognition sites. The presence of viral RNA was associated exclusively with the histopathological diagnosis of otosclerosis; the stapes specimens with negative measles virus belonged to non-otosclerotic stapes fixations. All specimens (N = 51) were characterized by the consecutive expression of five CD46 variants (c, d, e, f and one shorter unidentified isoform). Histologically confirmed ostosclerotic specimens (N = 21) were characterized by increased expression levels of variant "f" and the unknown isoform. Increased expression levels of these isoforms and special CD46 expression pattern of the human otic capsule might produce modified or pathological intracellular signalization that could create the possibility of persistent measles virus infection.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban CD46 Otosclerosis Measles Virus RT-PCR Restriction analysis egyetemen (Magyarországon) készült közlemény
Megjelenés:	European Archives of Oto-Rhino-Laryngology. - 267 : 2 (2010), p. 219-226. -
További szerzők:	Szalmás Anita (1978-) (biológus, mikrobiológus, klinikai mikrobiológus) Kónya József (1964-) (szakorvos, klinikai mikrobiológus) Sziklai István (1954-) (fül-orr-gégész) Karosi Tamás (1979-) (fül-orr-gégész)
Pályázati támogatás:	OTKA PD75371 OTKA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM013837
Első szerző:	Karosi Tamás (fül-orr-gégész)
Cím:	Osteoprotegerin expression and sensitivity in otosclerosis with different histological activity / Karosi Tamás, Csomor Péter, Szalmás Anita, Kónya József, Petkó Mihály, Sziklai István
Dátum:	2011
ISSN:	0937-4477
Megjegyzések:	Otosclerosis is a complex bone dystrophy of the human otic capsule leading to conductive and sensorineural hearing loss. Since otosclerosis may, at least in part, be considered as an autoimmune-inXammatory disease, disturbed balance of TNF-alpha and osteoprotegerin (OPG) expression has been implicated in the pathological bone remodeling. It has been supposed that active otosclerosis is characterized by decreased or missing local OPG production with invariable OPG sensitivity of the otosclerotic foci. Ankylotic stapes footplates (n = 41) removed by stapedectomy were processed to histological examination, OPGspeciWc RT-PCR, tissue culturing and alkaline-phosphatase (AP) activity assessment, respectively. OPG concentration of serum specimens (n = 41) was measured by ELISA. Cortical bone fragments harvested from the external ear canal were used as negative controls of otosclerosis. Among 41 ankylotic stapes footplates, 22 active and 19 inactive otosclerosis cases were histologically diagnosed. OPG expression was signiWcantly lower (p < 0.001) in active otosclerosis compared to inactive cases. Osteoclast cultures originated from active otosclerotic foci showed a considerable susceptibility against external OPG dosage, which resulted in a signiWcant decrease of AP activity (p < 0.001). In contrast, OPG serum levels were in the normal range (5-100 ng/ml) indicating a non-systemic bone resorption. In conclusion, secondary decreased local OPG production might play an important role in the pathogenesis of otosclerotic bone remodeling disorder. As to previous and current results, decreased OPG sensitivity of lesion-forming cells should be excluded. These observations may indicate the potential role of recombinant OPG treatment in early stages of otosclerosis.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban otosclerosis
Megjelenés:	European Archives Of Oto-Rhino-Laryngology. - 268 : 3 (2011), p. 357-365. -
További szerzők:	Csomor Péter (1984-) (biotechnológus) Szalmás Anita (1978-) (biológus, mikrobiológus, klinikai mikrobiológus) Kónya József (1964-) (szakorvos, klinikai mikrobiológus) Petkó Mihály (1943-) (orvos, neurobiológus) Sziklai István (1954-) (fül-orr-gégész)
Pályázati támogatás:	PD 75371 OTKA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: