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001-es BibID:BIBFORM060767
Első szerző:Nemes Z.
Cím:Expression and activation of tissue transglutaminase in apoptotic cells of involuting rodent mammary tissue / Z. Nemes, R. R. Friis, D. Aeschlimann, S. Saurer, M. Paulsson, L. Fésüs
Dátum:1996
ISSN:0171-9335
Megjegyzések:Apoptosis is a form of cell death in which cellular integrity is maintained and neither cytoplasmic nor nuclear content is released. The Ca(2+)-dependent tissue transglutaminase (tTG) is an enzyme that forms protein cross-links between specific glutamyl and lysyl side-chains of intra- and extracellular proteins, therefore it may be responsible for the structural stabilization observed during the death process. In this study, the expression of tTG was investigated following the physiological process of forced weaning which results in an almost complete elimination of secretory epithelium by apoptosis and remodelling of the tissue structure. A dramatic induction of tTG was detected by immunoblotting of total mammary gland homogenates prepared from the involuting glands. The concentration of epsilon(gamma-glutamyl)-lysine crosslinks was also elevated in these samples, showing that the enzyme is activated during apoptosis. To determine the distribution of tTG and its relationship to apoptotic cells, paraffin-embedded specimens were studied by RNA in situ hybridization and immunohistochemical methods as well as using in situ labeling for nuclear fragmentation. All of these approaches indicated that mammary secretory epithelium expressed tissue transglutaminase coincident with the onset of apoptosis. The apoptotic and tTG-expressing cells were found to be identical as demonstrated by a histological double-labeling technique.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:European Journal of Cell Biology 70 : 2 (1996), p. 125-133. -
További szerzők:Friis, Bob R. R. Aeschlimann, Daniel Saurer, S. Paulsson, M. Fésüs László (1947-) (orvos biokémikus)
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2.

001-es BibID:BIBFORM002765
Első szerző:Petrovski, Goran (orvos)
Cím:Clearance of dying autophagic cells of different origin by professional and non-professional phagocytes / Petrovski G., Zahuczky G., Katona K., Vereb G., Martinet W., Nemes Z., Bursch W., Fesus L.
Dátum:2007
Megjegyzések:MCF-7 cells undergo autophagic death upon tamoxifen treatment. Plated on non-adhesive substratum these cells died by anoikis while inducing autophagy as revealed by monodansylcadaverine staining, elevated light-chain-3 expression and electron microscopy. Both de novo and anoikis-derived autophagic dying cells were engulfed by human macrophages and MCF-7 cells. Inhibition of autophagy by 3-methyladenine abolished engulfment of cells dying through de novo autophagy, but not those dying through anoikis. Blocking exposure of phosphatidylserine (PS) on both dying cell types inhibited phagocytosis by MCF-7 but not by macrophages. Gene expression profiling showed that though both types of phagocytes expressed full repertoire of the PS recognition and signaling pathway, macrophages could evolve during engulfment of de novo autophagic cells the potential of calreticulin-mediated processes as well. Our data suggest that cells dying through autophagy and those committing anoikis with autophagy may engage in overlapping but distinct sets of clearance mechanisms in professional and non-professional phagocytes
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Apoptosis
Biophysics
Cells
Gene Expression
Human
Hungary
Macrophages
Microscopy
Phagocytosis
Research
Support
Megjelenés:Cell Death Differentiation. - 14 : 6 (2007), p. 1117-1128. -
További szerzők:Zahuczky Gábor (1975-) (molekuláris biológus, biokémikus, vegyész) Katona Klára (1976-) Vereb György (1965-) (biofizikus, orvos) Martinet, Wim Nemes Z. Bursch, Wilfried Fésüs László (1947-) (orvos biokémikus)
Internet cím:DOI
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