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001-es BibID:	BIBFORM072791
Első szerző:	Barta Tünde
Cím:	Endothelin-1-induced hypertrophic alterations and heme oxygenase-1 expression in cardiomyoblasts are counteracted by beta estradiol : in vitro and in vivo studies / Tunde Barta, Agnes Tosaki, David Haines, Gyorgy Balla, Istvan Lekli, Arpad Tosaki
Dátum:	2018
ISSN:	0028-1298
Megjegyzések:	Endothelin-1(ET-1),apotentvasoconstrictornormallyactiveinmaintainingvasculartone,maymediatesignificantpathogeniceffects, contributing to several serious diseases when aberrantly expressed or regulated. The present study evaluates the capacity of ET-1 to affect endothelin-1-associated hypertrophic activity and decreased expression of heme oxygenase-1 by H9c2 rat cardiomyoblasts in vitro, corresponding to in vivo processes underlying cardiovascular diseases (CVDs). Beta estradiol (?-E) is tested for its capacity to altertheeffectsofET-1.H9c2cells,cultured48h,werestimulatedwith100?10,000 nM of ET-1andevaluatedfor changes incell size,cellviability,andexpressionofthecytoprotectiveheatshockproteinhemeoxygenase-1(HO-1),with200nMof?-Eincludedin selectedculturestoevaluateitseffectonET-1-mediatedchanges.Theapplicationof100to10,000nMofET-1resultedinasignificant increase in average cell size and decreases in both cell viability and HO-1 protein content (p<0.05). Moreover, 200 nM of ?-E was observedtosignificantlycounteracttheseeffectsbycardiomyoblastsstimulatedwith1000nMofET-1(p<0.05).Sprague-Dawleyrats treated intravenously with 1000 ng/kg of ET-1 demonstrated reduced HO-1 expression in peripheral blood and left ventricular tissue, whichwascounteractedbyinjectionof200ng/kg?-E?demonstratingapossiblecorrespondencebetweeninvitroandinvivoeffects. Anoutcomeofparticularvalueforclinicaluseof ?-E,inthemanagementofcardiachypertrophy,istheobservedcapacityofthedrug to abate ET-1-mediated suppression of HO-1 expression. It has been previously demonstrated that HO-1 inducers exhibit potent cardioprotective properties, thus offering the promise of combining them with ?-E, allowing lower effective dosage of the drug and concomitantly lower adverse side effects associated with its clinical use. Major findings of this investigation are that pretreatment of cardiomyoblasts with ?-E inhibited their hypertrophic response to ET-1 and counteracts the decrease of cell viability. These effects were associated with a restoration of HO-1 protein expression in both under in vitro and in vivo conditions.
Tárgyszavak:	Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban endothelin H9c2 Beta-estradiol
Megjelenés:	Naunyn-Schmiedebergs Archives Of Pharmacology. - 391 : 4 (2018), p. 371-383. -
További szerzők:	Tósaki Ágnes (1992-) (bőrgyógyász) Haines, David Donald (1981-) (gyógyszerész) Balla György (1953-) (csecsemő és gyermekgyógyász, neonatológus) Lekli István (1981-) (gyógyszerész) Tósaki Árpád (1958-) (kísérletes farmakológus, gyógyszerész)
Pályázati támogatás:	OTKA-104017 OTKA GINOP-2.3.2-15-2016-00043 GINOP K104017 OTKA NKFIH-124719 NKFIH PD-111794 OTKA TÁMOP-4.2.4. A/2-11-1-2012-0001 TÁMOP GINOP-2.3.2-15-2016-00043 GINOP
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001-es BibID:	BIBFORM004141
Első szerző:	Szappanos Henrietta (biológus, élettanász)
Cím:	The Penicillium chrysogenum-derived antifungal peptide shows no toxic effects on mammalian cells in the intended therapeutic concentration / Szappanos H., Szigeti G. P., Pal B., Rusznak Z., Szucs G., Rajnavolgyi E., Balla J., Balla G., Nagy E., Leiter E., Pocsi I., Marx F., Csernoch L.
Dátum:	2005
Megjegyzések:	Certain filamentous fungi, such as the penicillin-producing strain Penicillium chrysogenum, secrete small, highly basic and cysteine-rich proteins with antifungal effects. Affected fungi include a number of important zoopathogens, including those infecting humans. Recent studies, however, have pointed to a membrane-perturbing effect of these antifungal compounds, apparent as a potassium efflux from affected fungal cells. If present on mammalian cells, this would severely hinder the potential therapeutic use of these molecules. Here we studied the effects of the P. chrysogenum-derived antifungal peptide (PAF) on a number of mammalian cells to establish whether the protein has any cytotoxic effects, alters transmembrane currents on excitable cells or activates the immune system. PAF, in a concentration range of 2?100 g/ml, did not cause any cytotoxicity on human endothelial cells from the umbilical vein. Applied at 10 g/ml, it also failed to modify voltage-gated potassium channels of neurones, skeletal muscle fibers, and astrocytes. PAF also left the hyperpolarization-activated non-specific cationic current (Ih) and the L-type calcium current unaffected. Finally, up to 2 g/ml, PAF did not induce the production of pro-inflammatory cytokines such as IL-6, IL-8, and TNF-. These results suggest that PAF should have only minor, if any, effects on mammalian cells in the intended therapeutic concentration range.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Naunyn-Schmiedeberg's archives of pharmacology. - 371 : 2 (2005), p. 122-132. -
További szerzők:	Szigeti Gyula (1969-) (élettanász, elektrofiziológus) Pál Balázs (1975-) (élettanász) Rusznák Zoltán (1965-) (élettanász) Szűcs Géza (1948-) (élettanász) Rajnavölgyi Éva (1950-) (immunológus) Balla József (1959-) (belgyógyász, nephrológus) Balla György (1953-) (csecsemő és gyermekgyógyász, neonatológus) Nagy Emőke (neonatológus, gyermekgyógyász) Leiter Éva (1976-) (biológus) Pócsi István (1961-) (vegyész) Marx, Florentine Csernoch László (1961-) (élettanász)
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