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001-es BibID:	BIBFORM092558
035-os BibID:	(cikkazonosító)1407 (WoS)000634343500001 (Scopus)85102714615
Első szerző:	Bukva Mátyás
Cím:	Raman Spectral Signatures of Serum-Derived Extracellular Vesicle-Enriched Isolates May Support the Diagnosis of CNS Tumors / Matyas Bukva, Gabriella Dobra, Juan Gomez-Perez, Krisztian Koos, Maria Harmati, Edina Gyukity-Sebestyen, Tamas Biro, Adrienn Jenei, Sandor Kormondi, Peter Horvath, Zoltan Konya, Almos Klekner, Krisztina Buzas
Dátum:	2021
ISSN:	2072-6694
Megjegyzések:	Investigating the molecular composition of small extracellular vesicles (sEVs) for tumor diagnostic purposes is becoming increasingly popular, especially for diseases for which diagnosis is challenging, such as central nervous system (CNS) malignancies. Thorough examination of the molecular content of sEVs by Raman spectroscopy is a promising but hitherto barely explored approach for these tumor types. We attempt to reveal the potential role of serum-derived sEVs in diagnosing CNS tumors through Raman spectroscopic analyses using a relevant number of clinical samples. A total of 138 serum samples were obtained from four patient groups (glioblastoma multiforme, non-small-cell lung cancer brain metastasis, meningioma and lumbar disc herniation as control). After isolation, characterization and Raman spectroscopic assessment of sEVs, the Principal Component Analysis?Support Vector Machine (PCA?SVM) algorithm was performed on the Raman spectra for pairwise classifications. Classification accuracy (CA), sensitivity, specificity and the Area Under the Curve (AUC) value derived from Receiver Operating Characteristic (ROC) analyses were used to evaluate the performance of classification. The groups compared were distinguishable with 82.9?92.5% CA, 80?95% sensitivity and 80?90% specificity. AUC scores in the range of 0.82?0.9 suggest excellent and outstanding classification performance. Our results support that Raman spectroscopic analysis of sEV-enriched isolates from serum is a promising method that could be further developed in order to be applicable in the diagnosis of CNS tumors.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Cancers. - 13 : 6 (2021), p. 1407-1426. -
További szerzők:	Dobra Gabriella Gomez-Perez, Juan Koós Krisztián Harmati Mária Gyukity-Sebestyén Edina Bíró Tamás (1968-) (élettanász) Jenei Adrienn (1978-) (biológus, kémikus) Kormondi Sándor Horváth Péter Kónya Zoltán (Szeged) Klekner Álmos (1970-) (idegsebész) Buzás Krisztina
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001-es BibID:	BIBFORM113378
035-os BibID:	(Scopus)85147867513 (WOS)000929367600001 (cikkazonosító)712
Első szerző:	Dobra Gabriella
Cím:	MMP-9 as Prognostic Marker for Brain Tumours : A Comparative Study on Serum-Derived Small Extracellular Vesicles / Dobra Gabriella, Gyukity-Sebestyén Edina, Bukva Mátyás, Harmati Mária, Nagy Valentina, Szabó Zoltán, Pankotai Tibor, Klekner Álmos, Buzás Krisztina
Dátum:	2023
ISSN:	2072-6694
Megjegyzések:	Simple Summary The invasive nature of brain tumours, particularly glioblastoma, severely limits its therapy. Matrix-metalloproteinases (MMPs), enzymes involved in the degradation of the extracellular matrix, are associated with the invasiveness of brain tumours; hence, the determination of MMPs is critical for the monitoring of cancer patients. The aim of our comparative study was to evaluate the possible additional utility of the MMP-9 level of serum-derived small extracellular vesicles (sEVs) for characterising brain tumours. We established a relationship between low MMP-9 content in sEVs and improved survival, and discovered that MMP-9 levels considerably differed between tumour types and stages, showing a positive correlation with aggressiveness. We demonstrated on a large number of samples that the high MMP-9 level of serum-sEVs may serve as a negative prognostic marker for brain tumours. Matrix metalloproteinase-9 (MMP-9) degrades the extracellular matrix, contributes to tumour cell invasion and metastasis, and its elevated level in brain tumour tissues indicates poor prognosis. High-risk tissue biopsy can be replaced by liquid biopsy; however, the blood-brain barrier (BBB) prevents tumour-associated components from entering the peripheral blood, making the development of blood-based biomarkers challenging. Therefore, we examined the MMP-9 content of small extracellular vesicles (sEVs)-which can cross the BBB and are stable in body fluids-to characterise tumours with different invasion capacity. From four patient groups (glioblastoma multiforme, brain metastases of lung cancer, meningioma, and lumbar disc herniation as controls), 222 serum-derived sEV samples were evaluated. After isolating and characterising sEVs, their MMP-9 content was measured by ELISA and assessed statistically (correlation, paired t-test, Welch's test, ANOVA, ROC). We found that the MMP-9 content of sEVs is independent of gender and age, but is affected by surgical intervention, treatment, and recurrence. We found a relation between low MMP-9 level in sEVs (<28 ppm) and improved survival (8-month advantage) of glioblastoma patients, and MMP-9 levels showed a positive correlation with aggressiveness. These findings suggest that vesicular MMP-9 level might be a useful prognostic marker for brain tumours.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk liquid biopsy small extracellular vesicles matrix metalloproteinase-9 central nervous system diseases brain tumour prognostic marker survival glioblastoma
Megjelenés:	Cancers. - 15 : 3 (2023), p. 1-21. -
További szerzők:	Gyukity-Sebestyén Edina Bukva Mátyás Harmati Mária Nagy Valentina Szabó Zoltán (orvos, Szeged) Pankotai Tibor Klekner Álmos (1970-) (idegsebész) Buzás Krisztina
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