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001-es BibID:	BIBFORM116643
035-os BibID:	(cikkazonosító)464286 (Scopus)85167593174 (WoS)001055045200001
Első szerző:	Andrási Melinda (gyógyszerész)
Cím:	Comparative study on the deamidation of three recombinant human insulins using capillary electrophoresis / M. Andrasi, G. Vishwakarma, R. Szabo, C. Nagy, A. Gaspar
Dátum:	2023
ISSN:	0021-9673
Megjegyzések:	The applicability of capillary zone electrophoresis (CZE) for the separation of different recombinant human insulins and their deamidated isoforms was studied. The high resolving power of CZE is demonstrated by its ability to separate insulin isoforms differing only by 0.984 Da (different-fold deamidated forms) and even components having the exacts same mass but slightly different shapes (same-fold deamidated forms). From among the several insulins available, humulin, glargine and glulisine were selected for our study because their sequences and chemical parameters are quite similar, however, the small differences present in their amino acid sequences influence the deamidation processes. Using a background electrolyte with basic pH was favourable not only for the separation of the different types of insulin but also for the separation of deamidated protein forms even in a bare fused silica capillary. The LOD values ranged between 0.6 - 0.93 mg/L and 2.17 - 4.37 mg/L for UV and ESI-MS detection, respectively. At -20 - -80 ?C, the deamidation is minimal, but at temperatures above +5 ?C deamidation is accelerated. At +5 ?C only 1-fold deamidation forms could be observed for each insulin. Acidified samples incubated for 1-month at room temperature showed varying levels of deamidation: 1-fold, 1?2-fold and 1?2?3-fold forms for glargine, glulisine and humulin, respectively.
Tárgyszavak:	Természettudományok Kémiai tudományok magyar nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Insulins Deamidation Isoforms Capillary electrophoresis Mass spectrometry
Megjelenés:	Journal of Chromatography A. - 1706 (2023), p. 1-8. -
További szerzők:	Vishwakarma, G. Szabó Ruben (1998-) Nagy Cynthia (1994-) Gáspár Attila (1970-) (vegyész, kémikus)
Pályázati támogatás:	K 142134 Egyéb ÚNKP-22-2-I-DE-193 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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