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1.

001-es BibID:BIBFORM044121
035-os BibID:PMID:9105639
Első szerző:Azerad, M. A.
Cím:Recirculated normal platelets adhere to surfaces coated with plasma from patients with immune thrombocytopenia / M. A. Azerad, J. Harsfalvi, H. Deckmyn, J. Vermylen, J. L. Michaux, M. F. Hoylaerts
Dátum:1997
ISSN:0957-5235
Megjegyzések:Immune thrombocytopenic purpura (ITP) patients have characteristic anti-platelet antibodies in their circulation. To assess the interaction between such antibodies adhering on to a non-physiological surface and human platelets, normal anticoagulated blood was perfused over ITP patient plasma-coated surfaces in a parallel plate flow chamber. At 300 s-1, platelet adhesion to patient plasma-coated glass coverslips (24.0 +/- 10%) was significantly higher than the adhesion to normal plasma-coated surfaces (9.8 +/- 7%). When perfused at 1300 s-1, the adhesion to patient plasma-(5.1 +/- 1.3%) and to normal plasma-(2.5 +/- 1.2%) coated coverslips were significantly weaker. Furthermore, patient platelet binding depended on simultaneous contributions by antibodies and fibrinogen present on the plasma-coated surface, since adherence was antagonized both by normal immunoglobulins added to the perfusate, as well as by the anti-GPIIb/IIIa monoclonal antibody 16N7C2, which competes with fibrinogen for binding to its receptor on the platelet. Accordingly, platelet adhesion was only observed to coverslips coated with the plasma but not the serum of ITP patients. Hence, perfusion of normal platelets over surfaces coated with ITP patient plasma enables a functional assessment of the presence in this plasma of anti-platelet antibodies.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Blood Coagulation & Fibrinolysis. - 8 : 1 (1997), p. 59-64. -
További szerzők:Hársfalvi Jolán (1949-) (klinikai biokémikus) Deckmyn, Hans Vermylen, Jozef Michaux, J. L. Hoylaerts, M. F.
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2.

001-es BibID:BIBFORM034614
035-os BibID:PMID:1799664
Első szerző:Boda Zoltán (belgyógyász, haematologus, klinikai onkológus)
Cím:Treatment of the severe bleeding episode in type III von Willebrand's disease by simultaneous administration of cryoprecipitate and platelet concentrate : case report / Z. Boda, G. Pfliegler, J. Hársfalvi, K. Rak
Dátum:1991
ISSN:0957-5235
Megjegyzések:A severe, life-threatening bleeding episode in a 24-year-old woman suffering from type III von Willebrand's disease was treated by large doses of cryoprecipitate with unsatisfactory results. Bleeding ceased and the bleeding time normalized only after concomitant administration of platelet concentrates. In the treatment of von Willebrand's disease patients possessing platelets with absent or insufficient von Willebrand factor activity the administration of plasma concentrates together with platelets appears to be justified.
Tárgyszavak:Orvostudományok Klinikai orvostudományok esettanulmány
vWF type III
platelet vWF activity
cryoprecipitate
platelet substitution
combined treatment
egyetemen (Magyarországon) készült közlemény
Megjelenés:Blood Coagulation & Fibrinolysis. - 2 : 6 (1991), p. 775-777. -
További szerzők:Pfliegler György (1949-) (belgyógyász, hematológus, labor szakorvos) Hársfalvi Jolán (1949-) (klinikai biokémikus) Rák Kálmán (1929-2005) (belgyógyász, klinikai hematológus, véralvadás kutató)
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3.

001-es BibID:BIBFORM043577
Első szerző:Cauwenberghs, Nancy
Cím:Epitope mapping of inhibitory antibodies against platelet glycoprotein Ibalpha reveals interaction between the leucine-rich repeat N-terminal and C-terminal flanking domains of glycoprotein Ibalpha / Cauwenberghs N., Vanhoorelbeke K., Vauterin S., Westra D. F., Romo G., Huizinga E. G., Lopez J. A., Berndt M. C., Harsfalvi J., Deckmyn H.
Dátum:2001
ISSN:0006-4971
Megjegyzések:The interaction of von Willebrand factor (vWF) with the platelet receptor glycoprotein Ibalpha (GPIbalpha) is important for platelet adhesion at high shear stress. Two functionally important antigenic areas within GPIbalpha were identified through the characterization of 5 new inhibitory anti-GPIb monoclonal antibodies (mAbs). The binding sites of 3 of these anti-GPIb mAbs, which were intercompeting and potently inhibiting shear stress-induced binding of vWF, were mapped within the N-terminal amino acid (aa) 1-59 area by the use of canine-human chimeras. These antibodies, however, had little or no effect (approximately 40% inhibition) on the binding of vWF induced by either botrocetin or ristocetin. On the other hand, the anti-GPIb mAbs 24G10 and 6B4, which blocked GPIb-vWF binding under all conditions examined, bound to 2 different regions of GPIbalpha, aa 1-81 and aa 201-268, respectively. The epitope for 6B4 was further narrowed by phage display revealing 2 sets of peptide sequences aligning within aa 259-262 and aa 230-242. In the latter region of GPIbalpha, the gain-of-function platelet-type von Willebrand disease (PT-vWD) mutations have been identified. Alignment was partially confirmed because the binding of 6B4 to recombinant GPIbalpha fragments carrying either one of the PT-vWD mutations was considerably impaired but not completely abolished. In contrast, mAb 24G10 bound more strongly to mutant PT-vWD GPIbalpha. However, although 24G10 competed with 6B4 for binding to platelets, it bound to an epitope within aa 1-81 of GPIbalpha. In conclusion, 2 functionally important areas within GPIbalpha were identified: one localized within the leucine-rich repeat N-terminal aa 1-59 area and one composed of residues aa 1-81 in close contact with aa 201-268. Moreover, further support is provided for the existence of an intramolecular interaction between the N-terminal flanking (aa 1-81) and C-terminal flanking (aa 201-268) regions.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
glycoprotein Ibalpha
Megjelenés:Blood. - 98 : 3 (2001), p. 652-660. -
További szerzők:Vanhoorelbeke, Karen Vauterin, Stephan Westra, Douwe F. Romo, Gabriel Huizinga, Eric G. Lopez, José A. Berndt, Michael C. Hársfalvi Jolán (1949-) (klinikai biokémikus) Deckmyn, Hans
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DOI
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4.

001-es BibID:BIBFORM043586
Első szerző:Hársfalvi Jolán (klinikai biokémikus)
Cím:Calin from Hirudo medicinalis, an inhibitor of von Willebrand factor binding to collagen under static and flow conditions / Harsfalvi J., Stassen J. M., Hoylaerts M. F., Van Houtte E., Sawyer R. T., Vermylen J., Deckmyn H.
Dátum:1995
ISSN:0006-4971
Megjegyzések:Calin from the saliva of the medicinal leech, Hirudo medicinalis, is a potent inhibitor of collagen mediated platelet adhesion and activation. In addition to inhibition of the direct platelet-collagen interaction, we presently demonstrate that binding of von Willebrand to coated collagen can be prevented by Calin, both under static and flow conditions in agreement with the occurrence of binding of Calin to collagen, confirmed by Biospecific Interaction Analysis. To define whether Calin acted by inhibiting the platelet-collagen or the platelet-von Willebrand factor (vWF)-collagen-mediated thrombus formation, platelet adhesion to different types of collagens was studied in a parallel-plate flow chamber perfused with whole blood at different shear rates. Calin dose-dependently prevented platelet adhesion to the different collagens tested both at high- and low-shear stress. The concentration of Calin needed to cause 50% inhibition of platelet adhesion at high-shear stress was some fivefold lower than that needed for inhibition of vWF-binding under similar conditions, implying that at high-shear stress, the effect of Calin on the direct platelet-collagen interactions, suffices to prevent thrombus formation. Platelet adhesion to extracellular matrix (ECM) of cultured human umbilical vein endothelial cells was only partially prevented by Calin, and even less so at a high-shear rather than a low-shear rate, whereas the platelet binding to coated vWF and fibrinogen were minimally affected at both shear rates. Thus, Calin interferes with both the direct platelet-collagen interaction and the vWF-collagen binding. Both effects may contribute to the inhibition of platelet adhesion in flowing conditions, although the former seems to predominate.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Calin
Megjelenés:Blood. - 85 : 3 (1995), p. 705-711. -
További szerzők:Stassen, Jean-Marie Hoylaerts, M. F. Van Houtte, Elisabeth Sawyer, Roy T. Vermylen, Jozef Deckmyn, Hans
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5.

001-es BibID:BIBFORM034608
035-os BibID:PMID:2133212
Első szerző:Hársfalvi Jolán (klinikai biokémikus)
Cím:The use of polybrene for heparin neutralization in protein C activity assay / J. Harsfalvi, G. Pfliegler, M. Udvardy, Z. Boda, I. Tornai, K. Rak
Dátum:1990
ISSN:0957-5235
Megjegyzések:The protein C activity assay of Francis and Patch (Thromb Res 1983; 32: 605-613) is based on the prolongation of the activated partial thromboplastin time in the presence of activated protein C isolated from the test samples. The assay was modified and standardized by Rapaport et al. (Am J Clin Pathol 1987; 87: 491-497), but could still only be used in patients on heparin therapy after chromatographic removal of the heparin. In this study we attempted to eliminate the heparin separation step without losing the advantages of the modified (Rapaport) method. Heparin was added to the isolated protein C to obtain a rapid and complete antithrombin effect after the thrombin activation step and polybrene was subsequently used to neutralize the excess heparin. Using this modified assay protein C activity ranged from 67 to 133% in the normal population, and from 9 to 25% in coumarin-treated patients. Precision of the modified method was acceptable in both normal and pathological PC ranges: within- and between-batch variations were 5.6 and 3.6%, and 8 and 14%, respectively. The assay correlated well (r = 0.84) with the ELISA technique in both healthy donors and non-coumarin-treated patients.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Protein C assay
protein C deficiency
thrombosis
anticoagulant therapy
egyetemen (Magyarországon) készült közlemény
Megjelenés:Blood Coagulation & Fibrinolysis. - 1 : 4-5 (1990), p. 357-361. -
További szerzők:Pfliegler György (1949-) (belgyógyász, hematológus, labor szakorvos) Udvardy Miklós (1947-) (belgyógyász, haematológus) Boda Zoltán (1947-) (belgyógyász, haematologus, klinikai onkológus) Tornai István (1954-) (belgyógyász, gasztroenterológus) Rák Kálmán (1929-2005) (belgyógyász, klinikai hematológus, véralvadás kutató)
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6.

001-es BibID:BIBFORM004714
Első szerző:Novák Levente (biológus)
Cím:Shear-dependent morphology of von Willebrand factor bound to immobilized collagen / Novak, L., Deckmyn, H., Damjanovich, S., Harsfalvi, J.
Dátum:2002
Megjegyzések:We have developed an immunogold von Willebrand factor (VWF) detection method that permits almost complete coverage of individual VWF molecules, and by this unequivocal localization and morphologic analysis of collagen-bound VWF by atomic force microscopy (AFM). Perfusion of gel filtration-purified VWF in parallel plate perfusion chambers over glass coverslips coated with calf skin collagen, followed by AFM imaging in air, enabled us to assess possible morphologic differences between VWF bound at low (0.07 N/m(2) = 0.7 dynes/cm(2)) and high (4.55 N/m(2) = 45.5 dynes/cm(2)) shear stresses. No significant differences in VWF morphology were found, the molecules were oriented almost randomly, and there were no clear signs of VWF "uncoiling" either at a high or at a low shear regime. After perfusing 1 microg/mL VWF for 5 minutes, surface coverage at high shear was almost twice the one seen at low shear, and some larger and more irregularly shaped VWF molecules could be seen at high shear. This difference disappeared, however, at 15 minutes of perfusion and was probably caused by diffusion kinetics. Moreover, the presence of 68 x 10(9)/L washed fixed platelets in the perfusate did not have any visible effect on VWF morphology at high versus low shear stress. These findings suggest that shear stress does not influence significantly the overall molecular morphology of VWF during its binding to collagen-coated surface and are consistent with a constitutively expressed affinity of collagen-bound VWF for glycoprotein Ib.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Animal
Blood Platelets
Cattle
chemistry
Collagen Type I
Diffusion
Human
Hungary
Immunohistochemistry
Kinetics
metabolism
methods
Microscopy
Microscopy,Atomic Force
pathology
Perfusion
Platelet Adhesiveness
Protein Binding
Stress,Mechanical
Support,Non-U.S.Gov't
ultrastructure
von Willebrand Factor
Megjelenés:Blood. - 99 : 6 (2002), p. 2070-2076. -
További szerzők:Deckmyn, Hans Damjanovich Sándor (1936-2017) (biofizikus) Hársfalvi Jolán (1949-) (klinikai biokémikus)
Internet cím:elektronikus változat
DOI
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7.

001-es BibID:bibEBI00018588
Első szerző:Pósán Emőke (belgyógyász, kardiológus)
Cím:Thrombotic and fibrinolytic alterations in the aseptic necrosis of femoral head / Posan E., Szepesi K., Gáspár L., Csernátony Z., Hársfalvi J., Ajzner É., Tóth A., Udvardy M.
Dátum:2003
Megjegyzések:Recent reports seem to support the role of the thrombophilia and decreased fibrinolysis in the aetiopathogenesis of aseptic necrosis of bone. In the present study, haemostatic disturbances were analysed in adults (n = 49) and patients in childhood (Perthes disease) (n = 47) with aseptic necrosis of the femoral head. Fibrinolytic parameters (in vitro clot lysis, plasminogen, plasmatic plasminogen activator inhibitor-1 activity, D-dimer) along with lipoprotein (a) [Lp(a)] and fibrinogen were measured. von Willebrand factor, platelet activation and some thrombophilic factors (activated protein C resistance and factor V Leiden mutation, protein C, protein S activity) were also determined. Impaired fibrinolysis, an increased Lp(a) level along with slow clot lysis and increased platelet activation were found in adult cases. We detected five cases of factor V Leiden mutations (one heterozygotic and four homozygotic) among patients with Perthes disease. The clinical course of the heterozygous case was similar to the usual form of Perthes disease. The most severe form of Perthes disease has been observed in homozygous factor V Leiden mutation cases. The mutation of factor V Leiden per se probably does not induce the development of aseptic necrosis of bone tissue in childhood, but it does play a role in its acceleration. Homozygous factor V Leiden mutation definitely runs a more severe course. On the other hand, in adult cases, the disturbances of haemostasis, impaired fibrinolysis, elevated Lp(a) level, increased platelet activation and slight elevation of fibrinogen might have clinical relevance. Further studies should focus on proving the role of the haemostatic alterations in the pathogenesis of severe forms of aseptic bone necrosis. The use of antithrombotic drugs in order to slow the process of aseptic necrosis also has to be addressed in future surveys.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Blood Coagulation and Fibrinolysis. - 14 : 3 (2003), p. 243-248. -
További szerzők:Szepesi Kálmán (1938-) (ortopéd sebész) Gáspár Levente (1948-) (ortopéd és baleseti sebész) Csernátony Zoltán (1959-2023) (ortopéd sebész, traumatológus) Hársfalvi Jolán (1949-) (klinikai biokémikus) Ajzner Éva (1968-) (laboratóriumi szakorvos) Tóth Anikó Udvardy Miklós (1947-) (belgyógyász, haematológus)
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8.

001-es BibID:BIBFORM078885
Első szerző:Radnay Zita (belgyógyász)
Cím:A New Approach to Predict the Chance of Infectious Complications Following Autologous Hematopoietic Stem Cell Transplantation : Mannose-Binding Lectin ELISA / Zita Radnay, Miklos Udvardy, Attila Kiss, Maria Papp, Laszlo Rejto, Jolan Harsfalvi, Tamas Masszi, Robert Szasz, Peter Batar, Gyula Remenyi, Bela Telek
Dátum:2011
ISSN:0006-4971
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
Megjelenés:Blood. - 118 : 21 (2011), p. 4492. -
További szerzők:Udvardy Miklós (1947-) (belgyógyász, haematológus) Kiss Attila (1942-) (belgyógyász, haematológus) Papp Mária (1975-) (belgyógyász, gasztroenterológus) Rejtő László (1963-) (belgyógyász, haematológus) Hársfalvi Jolán (1949-) (klinikai biokémikus) Masszi Tamás Szász Róbert (1972-) (belgyógyász, haematológus) Batár Péter (1969-) (belgyógyász, haematológus) Reményi Gyula (1969-) (belgyógyász, haematológus) Telek Béla (1948-) (belgyógyász, haematológus)
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Szerző által megadott URL
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9.

001-es BibID:BIBFORM035432
035-os BibID:PMID:8054463
Első szerző:Udvardy Miklós (belgyógyász, haematológus)
Cím:Cyclic relapses of thrombotic thrombocytopenic purpura / M. Udvardy, I. Borka, K. Racz, J. Harsfalvi, Z. Boda, K. Rak
Dátum:1994
ISSN:0957-5235
Megjegyzések:A 24-year-old male patient was first observed with full-blown acute thrombotic thrombocytopenic purpura in 1991. Complete remission was achieved with plasma and plasmapheresis therapy, but in spite of continuous corticosteroid and aspirin administration, thrombocytopenic (megakaryocytic) relapses were observed every 26-30 days. Splenectomy and danazol failed to prevent the recurrence of the disease. Surprisingly, cyclosporin A (5 mg/kg/day) administration resulted in a complete transitional remission, but after dose reduction a less regular pattern of repeated milder recurrences was observed. Cryopreserved plasma, obtained from the patient during remission also proved to be effective in treating the last two thrombocytopenic episodes.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
chronic relapsing thrombotic thrombocytopenic purpura (TTP)
Cyclosporin-A
plasmapheresis
egyetemen (Magyarországon) készült közlemény
Megjelenés:Blood Coagulation & Fibrinolysis. - 5 : 2 (1994), p. 305-307. -
További szerzők:Borka I. (Kecskemét) Rácz K. (belgyógyász) Hársfalvi Jolán (1949-) (klinikai biokémikus) Boda Zoltán (1947-) (belgyógyász, haematologus, klinikai onkológus) Rák Kálmán (1929-2005) (belgyógyász, klinikai hematológus, véralvadás kutató)
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10.

001-es BibID:bibKLT00005075
Első szerző:Udvardy Miklós (belgyógyász, haematológus)
Cím:New pentamidine related substances which simultaneously inhibit platelet aggregation and accelerate plasmin generation and in vitro clot lysis / M. Udvardy, E. Posan, J. Hársfalvi, Z. Dinya
Dátum:1996
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Blood Coagulation and Fibrinolysis. - 7 : 2 (1996), 256-258. -
További szerzők:Pósán Emőke (1963-) (belgyógyász, kardiológus) Hársfalvi Jolán (1949-) (klinikai biokémikus) Dinya Zoltán (1942-) (vegyész)
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