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1.

001-es BibID:BIBFORM044121
035-os BibID:PMID:9105639
Első szerző:Azerad, M. A.
Cím:Recirculated normal platelets adhere to surfaces coated with plasma from patients with immune thrombocytopenia / M. A. Azerad, J. Harsfalvi, H. Deckmyn, J. Vermylen, J. L. Michaux, M. F. Hoylaerts
Dátum:1997
ISSN:0957-5235
Megjegyzések:Immune thrombocytopenic purpura (ITP) patients have characteristic anti-platelet antibodies in their circulation. To assess the interaction between such antibodies adhering on to a non-physiological surface and human platelets, normal anticoagulated blood was perfused over ITP patient plasma-coated surfaces in a parallel plate flow chamber. At 300 s-1, platelet adhesion to patient plasma-coated glass coverslips (24.0 +/- 10%) was significantly higher than the adhesion to normal plasma-coated surfaces (9.8 +/- 7%). When perfused at 1300 s-1, the adhesion to patient plasma-(5.1 +/- 1.3%) and to normal plasma-(2.5 +/- 1.2%) coated coverslips were significantly weaker. Furthermore, patient platelet binding depended on simultaneous contributions by antibodies and fibrinogen present on the plasma-coated surface, since adherence was antagonized both by normal immunoglobulins added to the perfusate, as well as by the anti-GPIIb/IIIa monoclonal antibody 16N7C2, which competes with fibrinogen for binding to its receptor on the platelet. Accordingly, platelet adhesion was only observed to coverslips coated with the plasma but not the serum of ITP patients. Hence, perfusion of normal platelets over surfaces coated with ITP patient plasma enables a functional assessment of the presence in this plasma of anti-platelet antibodies.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Blood Coagulation & Fibrinolysis. - 8 : 1 (1997), p. 59-64. -
További szerzők:Hársfalvi Jolán (1949-) (klinikai biokémikus) Deckmyn, Hans Vermylen, Jozef Michaux, J. L. Hoylaerts, M. F.
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2.

001-es BibID:BIBFORM034614
035-os BibID:PMID:1799664
Első szerző:Boda Zoltán (belgyógyász, haematologus, klinikai onkológus)
Cím:Treatment of the severe bleeding episode in type III von Willebrand's disease by simultaneous administration of cryoprecipitate and platelet concentrate : case report / Z. Boda, G. Pfliegler, J. Hársfalvi, K. Rak
Dátum:1991
ISSN:0957-5235
Megjegyzések:A severe, life-threatening bleeding episode in a 24-year-old woman suffering from type III von Willebrand's disease was treated by large doses of cryoprecipitate with unsatisfactory results. Bleeding ceased and the bleeding time normalized only after concomitant administration of platelet concentrates. In the treatment of von Willebrand's disease patients possessing platelets with absent or insufficient von Willebrand factor activity the administration of plasma concentrates together with platelets appears to be justified.
Tárgyszavak:Orvostudományok Klinikai orvostudományok esettanulmány
vWF type III
platelet vWF activity
cryoprecipitate
platelet substitution
combined treatment
egyetemen (Magyarországon) készült közlemény
Megjelenés:Blood Coagulation & Fibrinolysis. - 2 : 6 (1991), p. 775-777. -
További szerzők:Pfliegler György (1949-) (belgyógyász, hematológus, labor szakorvos) Hársfalvi Jolán (1949-) (klinikai biokémikus) Rák Kálmán (1929-2005) (belgyógyász, klinikai hematológus, véralvadás kutató)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM043711
Első szerző:Hársfalvi Jolán (klinikai biokémikus)
Cím:Enzymatic fluorimetric assay for [epsilon](gamma-glutamyl)lysine isodipeptides: a new assay for proteolytic fragments of proteins cross-linked by factor XIII and other transglutaminases / J. Harsfalvi, E. Tarcsa, M. Udvardy, L. Fesus
Dátum:1994
ISSN:0268-9499
Megjegyzések:A fluorescent method for the quantitation of ?(?-glutamyl)lysine isodipeptides is described which is applicable to the quantitation of the free isodipeptides in human plasma and in proteolytic digest of human platelet lysates. The possible diagnostic importance of free ?(?-glutamyl)lysine isodipeptides in plasma revealed the necessity of developing a relatively simple method suitable for the fast screening of large numbers of samples. Samples are deproteinised by Centrifree micro partition system, then purified by cation-exchange chromatography and lyophilised. Aliquots of the reconstituted samples are incubated with ?-glutamylamine cyclotransferase, the enzyme which specifically catalyses the conversion of ?(? glutamyl)lysine to free lysine and 5-oxo-L-praline. The L-lysine is decarboxylated and the cadaverin formed is extracted by pentane-l-ol. The primary amine produces a stable product with fluorescamine which is measured fluorimetrically. The ?(?-glutamyl)lysine isodipeptide standard curves were linear in a concentration range of 0?100 ?mol/l. Imprecision of measurement was 34% at 1.4 gmmol/l and 18% at 24.6?mol/l concentrations. Results correlated with the HPLC technique described previously (r=0.798). Median (and ranges) of the isodipeptide levels in plasma were 2.4 (0?9.2) gmmol/l (n=14) in apparently healthy individuals, 11.8 (0?80.7) ?mol/l(n=23) in patients with deep vein thrombosis and 0.9 (0.1?7.9) ?mol/l (n=6) in patients with acute myocardial infarction.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Enzymatic fluorimetric assay
Megjelenés:Fibrinolysis. - 8 : 6 (1994), p. 378-381. -
További szerzők:Tarcsa Edit Udvardy Miklós (1947-) (belgyógyász, haematológus) Fésüs László (1947-) (orvos biokémikus)
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DOI
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4.

001-es BibID:BIBFORM034608
035-os BibID:PMID:2133212
Első szerző:Hársfalvi Jolán (klinikai biokémikus)
Cím:The use of polybrene for heparin neutralization in protein C activity assay / J. Harsfalvi, G. Pfliegler, M. Udvardy, Z. Boda, I. Tornai, K. Rak
Dátum:1990
ISSN:0957-5235
Megjegyzések:The protein C activity assay of Francis and Patch (Thromb Res 1983; 32: 605-613) is based on the prolongation of the activated partial thromboplastin time in the presence of activated protein C isolated from the test samples. The assay was modified and standardized by Rapaport et al. (Am J Clin Pathol 1987; 87: 491-497), but could still only be used in patients on heparin therapy after chromatographic removal of the heparin. In this study we attempted to eliminate the heparin separation step without losing the advantages of the modified (Rapaport) method. Heparin was added to the isolated protein C to obtain a rapid and complete antithrombin effect after the thrombin activation step and polybrene was subsequently used to neutralize the excess heparin. Using this modified assay protein C activity ranged from 67 to 133% in the normal population, and from 9 to 25% in coumarin-treated patients. Precision of the modified method was acceptable in both normal and pathological PC ranges: within- and between-batch variations were 5.6 and 3.6%, and 8 and 14%, respectively. The assay correlated well (r = 0.84) with the ELISA technique in both healthy donors and non-coumarin-treated patients.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Protein C assay
protein C deficiency
thrombosis
anticoagulant therapy
egyetemen (Magyarországon) készült közlemény
Megjelenés:Blood Coagulation & Fibrinolysis. - 1 : 4-5 (1990), p. 357-361. -
További szerzők:Pfliegler György (1949-) (belgyógyász, hematológus, labor szakorvos) Udvardy Miklós (1947-) (belgyógyász, haematológus) Boda Zoltán (1947-) (belgyógyász, haematologus, klinikai onkológus) Tornai István (1954-) (belgyógyász, gasztroenterológus) Rák Kálmán (1929-2005) (belgyógyász, klinikai hematológus, véralvadás kutató)
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5.

001-es BibID:bibEBI00018588
Első szerző:Pósán Emőke (belgyógyász, kardiológus)
Cím:Thrombotic and fibrinolytic alterations in the aseptic necrosis of femoral head / Posan E., Szepesi K., Gáspár L., Csernátony Z., Hársfalvi J., Ajzner É., Tóth A., Udvardy M.
Dátum:2003
Megjegyzések:Recent reports seem to support the role of the thrombophilia and decreased fibrinolysis in the aetiopathogenesis of aseptic necrosis of bone. In the present study, haemostatic disturbances were analysed in adults (n = 49) and patients in childhood (Perthes disease) (n = 47) with aseptic necrosis of the femoral head. Fibrinolytic parameters (in vitro clot lysis, plasminogen, plasmatic plasminogen activator inhibitor-1 activity, D-dimer) along with lipoprotein (a) [Lp(a)] and fibrinogen were measured. von Willebrand factor, platelet activation and some thrombophilic factors (activated protein C resistance and factor V Leiden mutation, protein C, protein S activity) were also determined. Impaired fibrinolysis, an increased Lp(a) level along with slow clot lysis and increased platelet activation were found in adult cases. We detected five cases of factor V Leiden mutations (one heterozygotic and four homozygotic) among patients with Perthes disease. The clinical course of the heterozygous case was similar to the usual form of Perthes disease. The most severe form of Perthes disease has been observed in homozygous factor V Leiden mutation cases. The mutation of factor V Leiden per se probably does not induce the development of aseptic necrosis of bone tissue in childhood, but it does play a role in its acceleration. Homozygous factor V Leiden mutation definitely runs a more severe course. On the other hand, in adult cases, the disturbances of haemostasis, impaired fibrinolysis, elevated Lp(a) level, increased platelet activation and slight elevation of fibrinogen might have clinical relevance. Further studies should focus on proving the role of the haemostatic alterations in the pathogenesis of severe forms of aseptic bone necrosis. The use of antithrombotic drugs in order to slow the process of aseptic necrosis also has to be addressed in future surveys.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Blood Coagulation and Fibrinolysis. - 14 : 3 (2003), p. 243-248. -
További szerzők:Szepesi Kálmán (1938-) (ortopéd sebész) Gáspár Levente (1948-) (ortopéd és baleseti sebész) Csernátony Zoltán (1959-2023) (ortopéd sebész, traumatológus) Hársfalvi Jolán (1949-) (klinikai biokémikus) Ajzner Éva (1968-) (laboratóriumi szakorvos) Tóth Anikó Udvardy Miklós (1947-) (belgyógyász, haematológus)
Internet cím:DOI
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6.

001-es BibID:BIBFORM035432
035-os BibID:PMID:8054463
Első szerző:Udvardy Miklós (belgyógyász, haematológus)
Cím:Cyclic relapses of thrombotic thrombocytopenic purpura / M. Udvardy, I. Borka, K. Racz, J. Harsfalvi, Z. Boda, K. Rak
Dátum:1994
ISSN:0957-5235
Megjegyzések:A 24-year-old male patient was first observed with full-blown acute thrombotic thrombocytopenic purpura in 1991. Complete remission was achieved with plasma and plasmapheresis therapy, but in spite of continuous corticosteroid and aspirin administration, thrombocytopenic (megakaryocytic) relapses were observed every 26-30 days. Splenectomy and danazol failed to prevent the recurrence of the disease. Surprisingly, cyclosporin A (5 mg/kg/day) administration resulted in a complete transitional remission, but after dose reduction a less regular pattern of repeated milder recurrences was observed. Cryopreserved plasma, obtained from the patient during remission also proved to be effective in treating the last two thrombocytopenic episodes.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
chronic relapsing thrombotic thrombocytopenic purpura (TTP)
Cyclosporin-A
plasmapheresis
egyetemen (Magyarországon) készült közlemény
Megjelenés:Blood Coagulation & Fibrinolysis. - 5 : 2 (1994), p. 305-307. -
További szerzők:Borka I. (Kecskemét) Rácz K. (belgyógyász) Hársfalvi Jolán (1949-) (klinikai biokémikus) Boda Zoltán (1947-) (belgyógyász, haematologus, klinikai onkológus) Rák Kálmán (1929-2005) (belgyógyász, klinikai hematológus, véralvadás kutató)
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7.

001-es BibID:bibKLT00005075
Első szerző:Udvardy Miklós (belgyógyász, haematológus)
Cím:New pentamidine related substances which simultaneously inhibit platelet aggregation and accelerate plasmin generation and in vitro clot lysis / M. Udvardy, E. Posan, J. Hársfalvi, Z. Dinya
Dátum:1996
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Blood Coagulation and Fibrinolysis. - 7 : 2 (1996), 256-258. -
További szerzők:Pósán Emőke (1963-) (belgyógyász, kardiológus) Hársfalvi Jolán (1949-) (klinikai biokémikus) Dinya Zoltán (1942-) (vegyész)
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