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001-es BibID:BIBFORM033194
035-os BibID:PMID:22056526
Első szerző:Mendelboum Raviv, Shlomit (biológus)
Cím:Coating conditions matter to collagen matrix formation regarding von Willebrand factor and platelet binding / Shlomit Mendelboum Raviv, Katalin Szekeres-Csiki, Attila Jenei, Janos Nagy, Boris Shenkman, Naphtali Savion, Jolan Harsfalvi
Dátum:2012
ISSN:0049-3848
Megjegyzések:IntroductionVon Willebrand factor (VWF) and platelet binding needs a uniform collagen matrix therefore we aimed to find an optimal condition for the preparation of human type-I and type-III collagen matrices.MethodThe effects of pH, salt and ligand concentration and binding time were tested when collagen matrices were prepared by adsorption. Surface-bound collagen and collagen-bound VWF measured by specific antibodies. Platelet adhesion was tested under flow conditions at a shear rate of 1800 s? 1 for 2 min. Matrices and platelets were visualized by atomic force and scanning electron microscope.ResultsThe extent of human collagens type-I and III binding to the surface was 10 and 4 times greater and binding was maximal under 8?16 hours, when coated from physiological buffer solution versus acid solution. Collagen fibrils were more developed and platelet adhesion was higher, with more organized and denser aggregates. VWF binding was parallel to the surface bound collagen in both collagen types.ConclusionCollagen coating of surfaces for VWF binding and platelet adhesion studies is very variable from acid solution. Our experiments provide evidences that neutralizing the acid and adding NaCl in physiological concentration, thereby facilitating formation of collagen fibril molecules in solution, results in efficient coating of human type-I and type III collagens, which then bind normal VWF equally well.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Collagen matrix
Von Willebrand factor
Platelet adhesion
Thrombus formation
egyetemen (Magyarországon) készült közlemény
Megjelenés:Thrombosis Research. - 129 : 4 (2012), p. e29-e35. -
További szerzők:Szekeres-Csiki Katalin (1979-) (orvos) Jenei Attila (1966-) (biofizikus) Nagy János (biofizikus) Shenkman, Boris Savion, Naphtali Hársfalvi Jolán (1949-) (klinikai biokémikus)
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2.

001-es BibID:BIBFORM073777
Első szerző:Pályu Eszter
Cím:Major changes of von Willebrand factor multimer distribution in cirrhotic patients with stable disease or acute decompensation / Eszter Palyu, Jolan Harsfalvi, Tamas Tornai, Maria Papp, Miklos Udvardy, Katalin Szekeres-Csiki, Lajos Pataki, Karen Vanhoorelbeke, Hendrik B. Feys, Hans Deckmyn, Istvan Tornai
Dátum:2018
ISSN:0340-6245
Megjegyzések:Background and aims: There is an unstable balance between pro- and anti-haemostatic processes in patients with cirrhosis. We hypothesized, that in patients with acute decompensation (AD) the major alterations of von Willebrand factor (VWF) could contribute to the prothrombotic situation as compared to patients with stable (ST) cirrhosis. Methods: We analyzed different parameters of VWF, including detailed multimer distribution by densitometry and platelet adhesion together with ADAMTS13 activity and antigen and C-reactive protein (CRP) levels in patients with ST (n=99), with AD (n=54) and controls (n=92). Results: VWF antigen, ristocetin cofactor as well as collagen binding activities were elevated in both cirrhotic groups in a stepwise manner. There was a decrease in high and an increase in low molecular weight multimer ratios in the majority of ST cirrhosis. However, in 24 out of 54 AD patients ultra-large VWF multimers (ULMWM) were found. ADAMTS13 activity in ST and AD patients without ULMWM was similar to controls [median, (IQR)%: 98 (67-132) and 91 (60-110) vs. 106 (88-117), respectively]. The presence of ULMWM in AD patients was associated with low ADAMTS13 activity [33 (24-49)%] and high CRP level [23 (7.1-83.6)mg/l]. Adhesion of normal platelets showed a stepwise increase in the presence of cirrhotic plasmas, reaching the highest level in AD patients with ULMWM. Conclusion: Characteristic changes of VWF parameters are seen in ST cirrhosis. In AD patients highly increased VWF and reduced ADAMTS13 activity could be found, along with the presence of ULMWM, which are possible markers and contributors of the disease progression.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
cirrhosis
acute decompensation
systemic inflammation
von Willebrand factor multimers
trhombotic microangiopathy
Megjelenés:Thrombosis and Haemostasis. - 118 : 8 (2018), p. 1397-1408. -
További szerzők:Hársfalvi Jolán (1949-) (klinikai biokémikus) Tornai Tamás István (1984-) (belgyógyász) Papp Mária (1975-) (belgyógyász, gasztroenterológus) Udvardy Miklós (1947-) (belgyógyász, haematológus) Szekeres-Csiki Katalin (1979-) (orvos) Pataki Lajos Vanhoorelbeke, Karen Feys, Hendrik B. Deckmyn, Hans Tornai István (1954-) (belgyógyász, gasztroenterológus)
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3.

001-es BibID:BIBFORM043856
Első szerző:Szekeres-Csiki Katalin (orvos)
Cím:Von Willebrand-faktor és laboratóriumi diagnosztikai szerepe / Szekeres-Csiki Katalin, Udvardy Miklós László, Varga-Fekete Tímea, Hársfalvi Jolán
Dátum:2008
Tárgyszavak:Orvostudományok Elméleti orvostudományok magyar nyelvű folyóiratközlemény hazai lapban
Megjelenés:Orvostudományi Értesítő. - 81 : 1 (2008), p. 45-48. -
További szerzők:Udvardy Miklós László (1977-) (orvos, tudományos segédmunkatárs) Varga-Fekete Tímea Hársfalvi Jolán (1949-) (klinikai biokémikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:BIBFORM010455
Első szerző:Udvardy Miklós László (orvos, tudományos segédmunkatárs)
Cím:Novel evaluation method for densitometric curves of von Willebrand factor multimers and a new parameter (MMW) to describe the degree of multimersation / Udvardy, M. L., Szekeres-Csiki, K., Hársfalvi, J.
Dátum:2009
ISSN:0340-6245 (Print)
Megjegyzések:Von Willebrand factor (VWF) is built up from a varying number of subunits, of which the larger molecules have higher haemostatic activity. Von Willebrand disease (VWD) and thrombotic thrombocytopenic purpura are the best known disorders with pathognomonic changes of the highly multimerised VWF forms. There is an established method to calculate the relative amount of large oligomers. Our aim is to quantify the degree of VWF multimerisation as well, to complete the densitometric analysis of VWF electrophoresis. After optimisation, we have defined this new parameter (MMW) as the molecular weight corresponding to the lower boundary of the largest 25% of VWF protein. MMW was significantly different (p<.0001) in platelet lysate, normal samples and VWD type 2 samples (10.4, 6.3, 2.1, respectively). There was strong correlation between the MMW and the amount of large multimers in normal samples (r2=0.98) and in platelet lysate. However MMW was higher in platelet lysate, in which VWF is not cleaved by ADAMTS-13, than in healthy samples with the same amount of large multimers. Comparison of the new parameter and the collagen binding and ristocetin cofactor activity of VWF, showed that the functional tests are at least partially determined by the multimerisation; however, about 15% of VWD samples had normal activity to antigen ratios. The quantification of multimerisation aids the classification in these cases, especially at low antigen concentrations, and also might help in the detection of thrombotic conditions.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Von Willebrand disease
VWF multimer analysis
collagen
oligomer
ristocetin
von Willebrand factor
von Willebrand factor cleaving proteinase
ADAM protein
ADAMTS13 protein
human
buffer
multiprotein complex
analytic method
article
cell lysate
controlled study
densitometry
function test
hemostasis
human
human cell
molecular weight
polyacrylamide gel electrophoresis
priority journal
quantitative analysis
thrombotic thrombocytopenic purpura
von Willebrand disease
blood
case control study
chemistry
comparative study
evaluation
in vitro study
metabolism
methodology
protein binding
protein quaternary structure
statistics
ADAM Proteins
Buffers
Case-Control Studies
Collagen
Densitometry
Humans
Molecular Weight
Multiprotein Complexes
Protein Binding
Protein Structure
Quaternary
von Willebrand Disease
von Willebrand Factor
Megjelenés:Thrombosis and Haemostasis. - 102 : 2 (2009), p. 412-417. -
További szerzők:Szekeres-Csiki Katalin (1979-) (orvos) Hársfalvi Jolán (1949-) (klinikai biokémikus)
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Intézményi repozitóriumban (DEA) tárolt változat
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