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001-es BibID:	BIBFORM070872
Első szerző:	Mikóné Hamvas Márta (biológus)
Cím:	Cylindrospermopsin induces biochemical changes leading to programmed cell death in plants / M-Hamvas Márta, Ajtay Kitti, Beyer Dániel, Jámbrik Katalin, Vasas Gábor, Surányi Gyula, Máthé Csaba
Dátum:	2017
ISSN:	1360-8185 1573-675X
Megjegyzések:	In the present study we provide cytological and biochemical evidence that the cyanotoxin cylindrospermopsin (CYN) induces programmed cell death (PCD) symptoms in two model vascular plants: the dicot white mustard (Sinapis alba) and the monocot common reed (Phragmites australis). Cytological data include chromatin fragmentation and the increase of the ratio of TUNEL-positive cells in roots, the latter being detected in both model systems studied. The strongest biochemical evidence is the elevation of the activity of several single- stranded DNA preferring nucleases- among them enzymes active at both acidic and alkaline conditions and are probably directly related to DNA breaks occurring at the initial stages of plant PCD: 80 kDa nucleases and a 26 kDa nuclease, both having dual (single- and double-stranded nucleic acid) specificity. Moreover, the total protease activity and in particular, a 53-56 kDa alkaline protease activity increases. This protease could be inhibited by PMSF, thus regarded as serine protease. Serine proteases are detected in all organs of Brassicaceae (Arabidopsis) having importance in differentiation of specialized plant tissue through PCD, in protein degradation/processing during early germination and defense mechanisms induced by a variety of biotic and abiotic stresses. However, knowledge of the physiological roles of these proteases and nucleases in PCD still needs further research. It is concluded that CYN treatment induces chromatin fragmentation and PCD in plant cells by activating specific nucleases and proteases. CYN is proposed to be a suitable molecule to study the mechanism of plant apoptosis.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Cylindrospermopsin Sinapis alba Phragmites australis Single-strand-preferring nuclease Protease Programmed cell death
Megjelenés:	Apoptosis 22 : 2 (2017), p. 254-264. -
További szerzők:	Ajtay Kitti Beyer Dániel (1982-) (molekuláris biológus) Jámbrik Katalin Vasas Gábor (1975-) (biológus-vegyész) Surányi Gyula (1957-) (biológus) Máthé Csaba (1966-) (biológus)
Pályázati támogatás:	NKFIH Egyéb
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001-es BibID:	BIBFORM066833
035-os BibID:	(Scopus)85019678782 (WOS)000439311400002 (cikkazonosító)3525
Első szerző:	Resetár Anna
Cím:	Production and antioxidant capacity of tissue cultures from four Amaryllidaceae species / Anna Resetár, Csongor Freytag, Fruzsina Kalydi, Sándor Gonda, Márta M-Hamvas, Kitti Ajtay, László Papp, Csaba Máthé
Dátum:	2017
ISSN:	0001-6977
Megjegyzések:	The aim of this study was (i) to produce tissue cultures capable of efficient plant regeneration from European naturally occurring protected and/or pharmacologically important Amaryllidaceae species, and (ii) to test them for antioxidant activities in order to select tissue cultures that scavenge efficiently oxygen radicals. Bulb explants were collected from Galanthus woronowii, two Leucojum species and Sternbergia lutea- Leucojum species being Hungarian isolates. Mostly ?-naphthalene acetic acid (NAA) and benzyladenine (BA) were used as growth regulator combinations for the induction and maintenance of tissue cultures and further antioxidant activity studies. G. woronowii and L. vernum cultures produced shoots or whole plants via micropropagation (callus stage was observed only sporadically and callus tissue did not contribute to regeneration), whereas L. aestivum and S. lutea produced efficiently whole plants or multiple shoots via embryogenic calli. Total phenolic content, % inhibition of ABTS radical (ABTS) cation and peroxidase activities on native polyacrylamide gels were studied and showed differences between cultures. No relationship could be detected between polyphenol content/ radical scavenging capacities and H2O2 reducing enzyme activities. For G. woronowii, S. lutea and a culture line of L. vernum, polyphenol content and ABTS cation scavenging activities were high and for G. woronowii, comparable to organs of the native plants used as explant sources. Bulbs of native plants showed low radical scavenging activities in general. For L. vernum and L. aestivum tissue cultures grown in the presence of NAA as the sole growth regulator, ABTS* cation scavenging showed low values. Enzymatic antioxidant (pyrogallol peroxidase) activities were low for all cultures and organs of native plants. This study shows the species conservation value of these cultures and highlights the high antioxidant capacity of G. woronowii and S. lutea, attributed to the presence of non-enzymatic scavengers.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Amaryllidaceae micropropagation somatic embryogenesis free radical scavenging pyrogallol peroxidase
Megjelenés:	Acta Societatis Botanicorum Poloniae. - 86 : 1 (2017), p. 1-12. -
További szerzők:	Freytag Csongor (1993-) (biológus) Kalydi Fruzsina Gonda Sándor (1984-) (gyógyszerész) Mikóné Hamvas Márta (1963-) (biológus) Ajtay Kitti Papp László (1958-) (biológus, botanikus) Máthé Csaba (1966-) (biológus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI Kiadói változat
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