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001-es BibID:BIBFORM016491
Első szerző:Vig Attila
Cím:Development and characterization of a FIA system for selective assay of l-ascorbic acid in food samples / Attila Vig, Attila Igloi, Nora Adanyi, Gyongyi Gyemant, Csaba Csutoras and Attila Kiss
Dátum:2010
ISSN:1615-7591
Megjegyzések:An amperometric detector and an enzymatic reaction were combined for the measurement of l-ascorbic acid. The enzyme cell (containing immobilized ascorbate oxidase) was connected to a flow injection analyzer (FIA) system with a glassy carbon electrode as an amperometric detector. During optimization and measurements two sample injectors were used, one before and one after the enzyme cell, thus eliminating the background interferences. Subtraction of the signal area given in the presence of enzyme from the one given in the absence of enzyme was applied for measuring analyte concentrations and calibration at 400 mV. Analysis capacity of system is 25 samples/hour. The relative standard deviation (RSD) was below 5% (5 times repeated, 400 mu mol/L conc.), linearity up to 400 mu mol/L, limit of detection (LOD) 5 mu mol/L, fitting of calibration curve in 25-400 mu mol/L range was R (2) = 0.99.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Biosensor
Ascorbic acid
Amperometry
Glassy carbon
Flow injection
analysis
biosensor
oxidase
Megjelenés:Bioprocess and Biosystems Engineering. - 33 : 8 (2010), p. 947-952. -
További szerzők:Iglói Attila (1971-) (biológus-mikrobiológus) Adanyi Nóra Gyémánt Gyöngyi (1960-) (vegyész) Csutorás Csaba Kiss Attila
Internet cím:DOI
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001-es BibID:BIBFORM010543
Első szerző:Vig Attila
Cím:Impedimetric aflatoxin M-1 immunosensor based on colloidal gold and silver electrodeposition / Attila Vig, Antonio Radoi, Xavier Muñoz-Berbel, Gyongyi Gyemant, Jean-Louis Marty
Dátum:2009
ISSN:0925-4005
Megjegyzések:An impedimetric immunosensor based on colloidal gold and silver electrodeposition for the detection of aflatoxin M-1 (AFM(1)) herein is reported. An indirect like competitive ELISA procedure was performed on screen-printed electrodes (SPEs) in presence of anti-AFM(1) gold-labelled antibodies. Silver was chronoamperometrically electrode posited at a fixed applied potential and for a determined period of time to amplify the signal. The calculated charge transfer resistance (R-ct) was found to correlate well with the concentration of AFM(1). The linear working range of the described AFM(1) immunosensor ranged between 15 and 1000 ng/L with a limit of detection (LoD) equal to 15 ng/L (R.S.D. = 20%). Impedimetric results were confronted with linear sweep voltametry (LSV) and corresponded well to this technique.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Sensors and Actuators B-Chemical. - 138 : 1 (2009), p. 214-220. -
További szerzők:Radoi, Antonio Muñoz-Berbel, Xavier Gyémánt Gyöngyi (1960-) (vegyész) Marty, Jean-Louis
Internet cím:DOI
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