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001-es BibID:	BIBFORM079765
Első szerző:	Takács István (Szeged)
Cím:	HPLC method for measurement of human salivary α-amylase inhibition by aqueous plant extracts / Takács István, Takács Ákos, Pósa Anikó, Gyémánt Gyöngyi
Dátum:	2017
ISSN:	0236-5383 1588-256X
Megjegyzések:	Control of hyperglycemia is an important treatment in metabolic disorders such as type II diabetes and obesity. α-Amylase, as the first enzyme of glucose release from dietary polysaccharides, is a potential target to identify new sources of novel anti-obesity and anti-diabetic drugs. In this work, different herbal extracts as α-amylase inhibitors were studied by measuring the rate of the cleavage of a maltooligomer substrate 2-chloro-4-nitrophenyl-?-D-maltoheptoside. Measurement of chromophore containing products after reversed phase HPLC separation was used for α-amylase activity measurement. Rates of hydrolysis catalysed by human salivary α-amylase were determined in the presence and absence of lyophilised water extracts of eleven herbs. Remarkable bioactivities were found for extracts of Cinnamomum zeylanicum Blume (bark), Camellia sinensis L. (leaf), Ribes nigrum L. (leaf), Laurus nobilis L. (leaf), Vaccinium macrocarpon Aiton (fruit) and Syzygium aromaticum L. (bud). Determined IC50 values were in 0.017?41 ?g/ml range for these six selected plant extracts. Our results confirm the applicability of this HPLC-based method for the quick and reliable comparison of plants as α-amylase inhibitors.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény hazai lapban folyóiratcikk α-amylase inhibition plant extract HPLC obesity diabetes
Megjelenés:	Acta Biologica Hungarica. - 68 : 2 (2017), p. 127-136. -
További szerzők:	Takács Ákos Pósa Anikó Gyémánt Gyöngyi (1960-) (vegyész)
Pályázati támogatás:	ÚNKP-16-4 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM104110
035-os BibID:	(WOS)000551124000007 (Scopus)85088609147
Első szerző:	Takács István
Cím:	Wild Strawberry, Blackberry, and Blueberry Leaf Extracts Alleviate Starch-Induced Hyperglycemia in Prediabetic and Diabetic Mice / István Takács, András Szekeres, Ákos Takács, Dávid Rakk, Miklós Mézes, Ágnes Polyák, Lóránt Lakatos, Gyöngyi Gyémánt, Dezső Csupor, Krisztina J. Kovács, Szilamér Ferenczi
Dátum:	2020
ISSN:	0032-0943
Megjegyzések:	Intestinal alpha-glucosidase and alpha-amylase break down nutritional poly- and oligosaccharides to monosaccharides and their activity significantly contributes to postprandial hyperglycemia. Competitive inhibitors of these enzymes, such as acarbose, are effective antidiabetic drugs, but have unpleasant side effects. In our ethnopharmacology inspired investigations, we found that wild strawberry (Fragaria vesca), blackberry (Rubus fruticosus), and European blueberry (Vaccinium myrtillus) leaf extracts inhibit alpha-glucosidase and alpha-amylase enzyme activity in vitro and are effective in preventing postprandial hyperglycemiain vivo. Toxicology tests on H9c2 rat embryonic cardiac muscle cells demonstrated that berry leaf extracts have no cytotoxic effects. Oral administration of these leaf extracts alone or as a mixture to normal (control), obese, prediabetic, and streptozotocin-induced diabetic mice attenuated the starch-induced rise of blood glucose levels. The efficiency was similar to that of acarbose on blood glucose. These results highlight berry leaf extracts as candidates for testing in clinical trials in order to assess the clinical significance of their effects on glycemic control.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk diabetes alpha-glucosidase alpha-amylase Fragaria vesca Rubus fruticosus Vaccinium myrtillus Rosaceae Ericaceae
Megjelenés:	Planta Medica. - 86 : 11 (2020), p. 790-799. -
További szerzők:	Szekeres András Takács Ákos Rakk Dávid Mézes Miklós Polyák Ágnes Lakatos Lóránt Gyémánt Gyöngyi (1960-) (vegyész) Csupor Dezső Kovács Krisztina J. Ferenczi Szilamér (1977-) (biológus)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00012 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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