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001-es BibID:	BIBFORM111384
035-os BibID:	(cikkazonosító)150 (WoS)000958878700001 (Scopus)85151131747
Első szerző:	Andrási Melinda (gyógyszerész)
Cím:	Analysis of Sugars in Honey Samples by Capillary Zone Electrophoresis Using Fluorescence Detection / Melinda Andrasi, Gyongyi Gyemant, Zsofi Sajtos, Cynthia Nagy
Dátum:	2023
ISSN:	2297-8739
Megjegyzések:	: The applicability of capillary electrophoresis (CE) with light-emitting diode-induced fluorescence detection (LEDIF) for the separation of sugars in honey samples was studied. An amount of 25 mM ammonium acetate (pH 4.5) with 0.3% polyethylene oxide (PEO) was found to be optimal for the efficient separation of carbohydrates. 8-aminopyrene-1,3,6-trisulfonic acid (APTS) was used for the labeling of the carbohydrate standards and honey sugars for fluorescence detection. The optimized method was applied in the quantitative analysis of fructose and glucose by direct injection of honey samples. Apart from the labeling reaction, no other sample preparation was performed. The mean values of the fructose/glucose ratio for phacelia honey, acacia honey and honeydew honey were 0.86, 1.61 and 1.42, respectively. The proposed method provides high separation efficiency and sensitive detection within a short analysis time. Apart from the labeling reaction, it enables the injection of honeys without sample pretreatment. This is the first time that fluorescence detection has been applied for the CE analysis of sugars in honeys.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk capillary electrophoresis fluorescence detection honey sugars
Megjelenés:	Separations. - 10 : 3 (2023), p. 1-10-. -
További szerzők:	Gyémánt Gyöngyi (1960-) (vegyész) Sajtos Zsófi (1994-) (kémikus) Nagy Cynthia (1994-)
Pályázati támogatás:	K 127931 Egyéb K 142134 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM106976
035-os BibID:	(Scopus)85139781076 (cikkazonosító)100024
Első szerző:	Nagy Cynthia (1994-)
Cím:	Top-down proteomic analysis of monoclonal antibodies by capillary zone electrophoresis-mass spectrometry / Cynthia Nagy, Melinda Andrási, Narmin Hamidli, Gyöngyi Gyémánt, Attila Gáspár
Dátum:	2022
ISSN:	2772-3917
Megjegyzések:	Capillary zone electrophoresis (CZE) is considered an alternative to advanced chromatographic methods for the analysis of monoclonal antibodies (mAbs) as those are well applicable for the high-resolution separation of intact proteins, proteoforms or even protein complexes. Thus, CZE with mass spectrometry (MS) detection, has the potential to grow into a powerful analytical platform for the extensive investigation of mAbs. The top-down proteomic approach, where the application of CZE-MS might be exceptionally beneficial, provides the determination of both the accurate molecular mass and several microheterogeneities. Although there is a relatively small number of publications about the CZE-MS of mAbs, the pharmaceutical industry has an unambiguous interest on this field and thorough, intensive research has been initiated. In this review, we surveyed the developments of top-down CZE?MS applied for mAbs. The merits and limitations of the published capillary coatings and running electrolytes used for CZE-MS were discussed. The different aspects of CZE-MS hyphenation, furthermore, the applications of such mAb studies were surveyed, as well.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Journal of Chromatography Open. - 2 (2022), p. 1-21. -
További szerzők:	Andrási Melinda (1979-) (gyógyszerész) Hamidli, Narmin Gyémánt Gyöngyi (1960-) (vegyész) Gáspár Attila (1970-) (vegyész, kémikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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