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1.

001-es BibID:BIBFORM046289
Első szerző:Bacsó Zsolt (biofizikus)
Cím:A photobleaching energy transfer analysis of CD8MHC-I and LFA-1ICAM-1 interactions in CTL-target cell conjugates / Bacsó Zsolt, Bene László, Bodnár Andrea, Matkó János, Damjanovich Sándor
Dátum:1996
ISSN:0165-2478
Megjegyzések:The photobleaching energy transfer (pbFRET) technique is a fluorescence method to measure proximity relationships between molecules, especially cell surface proteins, labeled with fluorophore-conjugated monoclonal antibodies, on a pixel-by-pixel base using digital imaging microscopy. This technique enables analysis of inter- and intramolecular proximities at cell surfaces at physiological conditions. We have developed a pbFRET approach to measure intercellular proximities in order to access spatial organization of interacting proteins in the contact region of two 'communicating' cells. Two examples, as possible application areas of this approach, are presented here: interaction between CD8 and MHC-I molecules in point contacts and interaction between LFA-1 and ICAM-1 molecules in focal contacts of CTL-target conjugates. The geometry of these protein contacts based on our resonance energy transfer (RET) data is consistent with the observed blocking effects of monoclonal antibodies (directed against the interacting proteins) on the cytolytic activity of CTLs and suggest a critical role for CD8beta-subunit in signal transmission in peripheral T-lymphocytes
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Immunology Letters. - 54 : 2-3 (1996), p. 151-156. -
További szerzők:Bene László (1963-) (biofizikus) Dóczy-Bodnár Andrea (1970-) (biofizikus) Matkó János (1952-) (biológus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM082468
Első szerző:Bene László (biofizikus)
Cím:Adaptive threshold-stochastic resonance (AT-SR) in MHC clusters on the cell surface / László Bene, Miklós Bagdány, László Damjanovich
Dátum:2019
ISSN:0165-2478
Megjegyzések:Highly conserved 2D receptor clusters (membrane rafts) of immunological signaling molecules with MHCI and MHCII antigens as their cores have been observed in the past on the surface of T- and B-cell lines of lymphoid origin, as well as on cells from patients with colon tumor and Crohn's disease. Conservativity is related to the ever presence of MHCI molecules. Although they are suspected to play a role in maintaining these clusters and facilitating transmembrane signaling, their exact role has been left largely enigmatic. Here we are suggesting stochastic resonance (SR), or "noise-assisted signal detection", as a general organizing principle for transmembrane signaling events evoked by processes like immune recognition and cytokine binding taking place in these clusters. In the conceptual framework of SR, in immune recognition as a prototype of transmembrane signaling, the sea of self-peptide-MHC complexes around a nonself-peptide presenting MHC is conceived as a source of quickly fluctuating unspecific signal ("athermal noise") serving the extra energy for amplifying the weak sub-threshold specific signal of the nonself-peptide presenting MHC. This same noise is also utilized for a readjustment of the threshold ? and also the sensitivity and specificity ? of detection by a closed loop feedback control of the TcR-CD8 (CD4) proximity on the detecting T-cell. The weak sub threshold specific signal of nonself-peptide presenting MHC is amplified by the superposing unspecific signals of the neighboring self peptide-MHC complexes towards the T-cell receptor as the detector. Because in a successful detection event both self- and nonself-peptides are detected simultaneously, the principle of coincidence (or lock-in) detection is also realized. The ever presence of MHC islands gets a natural explanation as a source of extra power ? in a form of "athermal noise" ? needed for coincidence detection and frequency encoding the evoked downstream signals. The effect is quite general, because the actual type of molecules surrounding a chief signaling molecule ? like nonself-peptide holding MHC, interleukin-2 and -15 cytokine receptors (IL-2R/15R) ? as the fluctuating interaction energy sources is immaterial. The model applies also for other types of signaling, such as those evoked by cytokine binding. The phenomenon of SR can also be interpreted as sampling of a low frequency, specific signal with a high frequency unspecific signal, the "noise". Recipes for identifying other forms of SR in membrane clusters with biophysical tools are recommended.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Immunology Letters. - 217 (2019), p. 65-71. -
További szerzők:Bagdány Miklós Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
Sebészet Kutatócsoport
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM004703
035-os BibID:(scopus)0037013728 (wos)000176059200014
Első szerző:Damjanovich Sándor (biofizikus)
Cím:Does mosaicism of the plasma membrane at molecular and higher hierarchical levels in human lymphocytes carry information on the immediate history of cells? / Damjanovich, S., Matyus, L., Damjanovich, L., Bene, L., Jenei, A., Matko, J., Gaspar, R., Szollosi, J.
Dátum:2002
Megjegyzések:A theoretical analysis of experimental data is presented in this mini-review on non-random homo- and hetero-associations of cell surface receptors, which can be recruited in the plasma membrane or at the surface of the rough endoplasmic reticulum during the protein synthesis. In the latter case, the likely genetic origin of these supramolecular formations is analyzed, contrasting this concept to the mobility of the cell surface proteins. A model is offered which, on the one hand, allows the mobility in a restricted way even among microdomain-confined receptor proteins through 'swapping partners'. On the other hand, the lack of mixing molecular components of protein clusters will be analyzed, when homo-and hetero-associations are studied through cell fusion experiments. The most frequently studied cell surface patterns have included lipid raft organized HLA class I and II, ICAM-1, tetraspan molecules, IL2 and IL15 and other receptors, as well. On the contrary coated pit-associated transferrin receptors would not mix with the above lipid raft associated receptor patterns, although transferrin receptor would readily oligomerize into homo-associates. The functional consequences of these superstructures are also analyzed. On the 30th anniversary of the Singer-Nicolson fluid mosaic membrane model one has to pay tribute to the authors, because of their deep insight emphasizing also the mosaicism of the membranes in general and that of the plasma membrane, in particular.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
analysis
Biophysics
Cell Fusion
Cells
Human
Hungary
Lymphocytes
Proteins
egyetemen (Magyarországon) készült közlemény
Megjelenés:Immunology Letters. - 82 : 1-2 (2002), p. 93-99. -
További szerzők:Mátyus László (1956-) (biofizikus) Damjanovich László (1960-) (általános sebész) Bene László (1963-) (biofizikus) Jenei Attila (1966-) (biofizikus) Matkó János (1952-) (biológus) Gáspár Rezső (1944-) (biofizikus) Szöllősi János (1953-) (biofizikus)
Internet cím:DOI
elektronikus változat
Borító:

4.

001-es BibID:BIBFORM046291
Első szerző:Dóczy-Bodnár Andrea (biofizikus)
Cím:Modification of membrane cholesterol level affects expression and clustering of class I HLA molecules at the surface of JY human lymphoblasts / Bodnár Andrea, Jenei Attila, Bene László, Damjanovich Sándor, Matkó János
Dátum:1996
ISSN:0165-2478
Megjegyzések:Recently we have found that class I HLA molecules, key elements of the antigen presentation system for CD8 + effector cells, show a clustered lateral distribution (homoassociation) at the surface of activated human T- and B-lymphocytes as well as virus-transformed T- and B-lymphoblasts, in contrast to a disperse distribution on resting human PBLs (Matk6 et al. (1994) J. Immunol. 152, 3353; Bene et al. (1994) Eur. J. Immunol. 24, 2115). Expression of beta2m-free HLA heavy chains and exogenous beta2m have been shown as potential regulation factors of HLA-I clustering, which in turn may affect cytotoxic activity of CD8+ effector cells. Here we report a study on the effect of plasma membrane-modification (by exogenous cholesterol and phosphatidylcholine) on the expression of free HLA heavy chains and beta2m-bound HLA-I molecules on JY human B-lymphoblasts. The modulating effect of these two treatments on the lipid fluidity of cells was demonstrated by fluorescence anisotropy of DPH lipid probe. The lateral clustering (association) of HLA-I molecules was detected by flow cytometric fluorescence resonance energy transfer (FCET) and digital imaging microscopic photobleaching energy transfer (pbFRET) methods, using flourescein-isothiocyanate (FITC) (donor)- and tetramethyl-rhodamine-isothiocyanate (TRITC) (acceptor)-labeled W6/32 or KE2 antibodies directed against intact HLA-I molecules. Cholesterol enrichment of the plasma membrane increased membrane fluidity and reduced the expression of heavy- and light-chain determinants of HLA-I molecules and free heavy chains (FHCs). This was accompanied with a higher degree of HLA-I clustering as shown by the enhanced intermolecular energy transfer efficiency. In contrast, cholesterol depletion resulted in membrane fluidization and increased expression of HLA-I epitopes. Our results suggest that both cholesterol level and lipid structure/fluidity of the plasma membrane in lymphoblastoid cells may also potentially regulate lateral organization and consequently the presentation efficiency of HLA-I molecules.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Immunology Letters. - 54 : 2-3 (1996), p. 221-226. -
További szerzők:Jenei Attila (1966-) (biofizikus) Bene László (1963-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Matkó János (1952-) (biológus)
Pályázati támogatás:T6163
OTKA
6221
OTKA
17592
OTKA
F020102
OTKA
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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5.

001-es BibID:BIBFORM004646
Első szerző:Erdei Anna
Cím:Inhibition of IgE-mediated triggering of mast cells by complement-derived peptides interacting with the Fc epsilon RI / Erdei, A., Toth, G. K., Andrasfalvy, M., Matko, J., Bene, L., Bajtay, Z., Ischenko, A., Rong, X., Pecht, I.
Dátum:1999
Megjegyzések:Mucosal type mast cells, in contrast to the serosal type ones, do not respond to cationic agents, or to the complement-derived peptides C3a and C5a. Earlier we have found that while C3a does not activate the rat mucosal type mast cells (line RBL-2H3), it strongly inhibits the IgE-mediated triggering of these cells, by interfering with the Fc epsilon RI-initiated signaling pathway. In the present study we further investigated the mechanism of this process. It is shown, that C3a interacts with the beta-chain of the Fc epsilon RI complex. Binding of the complement peptide to the cells apparently causes a decrease in the proximity of the IgE-binding Fc epsilon RI. Investigating certain sequences of C3a we found that the inhibition is caused by the C-terminal sequences of the complement-peptide, ranging from positions 56 to 77 and also by a shorter sequence, ranging from positions 56 to 64. The inhibitory effect of these peptides was observed both in the case of RBL-2H3 cells and mouse bone marrow derived mast cells
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Amino Acid Sequence
Animal
Bone Marrow Cells
Cells
Cells,Cultured
chemistry
Complement
Complement 3a
Hungary
Immunoglobulin E
immunology
Immunosuppressive Agents
Mast Cells
metabolism
Mice
Molecular Sequence Data
Peptides
pharmacology
physiology
Protein Conformation
Rats
Receptors, IgE
Support, Non-U.S.Gov't
Megjelenés:Immunology Letters. - 68 : 1 (1999), p. 79-82. -
További szerzők:Tóth Gábor K. Andrásfalvy Márton Matkó János (1952-) (biológus) Bene László (1963-) (biofizikus) Bajtay Zsuzsa Ischenko, Alexander Rong, Xu Pecht, Israel
Internet cím:DOI
elektronikus változat
elektronikus változat
Borító:

6.

001-es BibID:BIBFORM004947
Első szerző:Kiss Erzsébet
Cím:Effect of TSH and anti-TSH receptor antibodies on the plasma membrane potential of polymorphonuclear granulocytes / Kiss, E., Balazs, C., Bene, L., Damjanovich, S., Matko, J.
Dátum:1997
ISSN:0165-2478
Megjegyzések:Effects of thyrotropin hormone (TSH) and anti-TSH receptor antibodies on the plasma membrane potential of polymorphonuclear granulocytes (PMN) were analyzed by means of flow cytometry. Both TSH and the autoantibody caused a rapid, dose-dependent hyperpolarization of the plasma membrane of PMNs. TSH was also able to mask (revert) the depolarizing effect of a chemotactic peptide, fMLP, on PMNs. No detectable rise in the cytosolic free calcium level accompanied the observed hyperpolarization. Quinine, a blocker of Ca(2+)-activated and voltage-gated K+ channels did not affect the hyperpolarization by TSH and antibodies. Decreasing the [K+] gradient across the plasma membrane by valinomycin, however, blocked the hyperpolarizing effect. Peptide362-376 (derived from the extracellular domain of TSH receptor) also blocked the hyperpolarization induced by both TSH and anti-TSHR antibodies. These data suggest that the observed hyperpolarization is a specific, receptor-mediated early signal during interaction of PMNs with TSH or anti-TSHR antibodies.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Amino Acid Sequence
Antibodies,Monoclonal
Autoantibodies
Calcium
Cell Membrane
chemistry
Dose-Response Relationship,Drug
drug effects
Flow Cytometry
Fluorescence Polarization
Human
Hungary
immunology
Membrane Potentials
metabolism
Molecular Sequence Data
N-Formylmethionine Leucyl-Phenylalanine
Neutrophils
Oligopeptides
pharmacology
physiology
Potassium
Potassium Channels
Quinine
Receptors,Thyrotropin
Support,Non-U.S.Gov't
Thyrotropin
ultrastructure
Valinomycin
Megjelenés:Immunology Letters. - 55 : 3 (1997), p. 173-177. -
További szerzők:Balázs Csaba Bene László (1963-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Matkó János (1952-) (biológus)
Internet cím:elektronikus változat
DOI
Borító:

7.

001-es BibID:BIBFORM046280
Első szerző:Mátyus László (biofizikus)
Cím:Distinct association of transferrin receptor with HLA class I molecules on HUT-102B and JY cells / Mátyus László, Bene László, Heiligen Harry, Rausch Jeff, Damjanovich Sándor
Dátum:1995
ISSN:0165-2478
Megjegyzések:The topological relationship of transferrin receptor (TfR) has been studied relative to the heavy and light chains of the HLA class I molecules, class II molecules, interleukin-2 receptor alpha-chain and ICAM-1 molecule in the plasma membrane of HUT-102B2 T and JY B lymphoblastoid cell lines using the flow cytometric fluorescence energy transfer technique (FCET). The effect of different growing conditions (logarithmic and plateau phases) on the relative surface density of the receptors and the lateral organization of the TfR was also studied. The TfR showed a high degree of self-association on the surface of both cell lines regardless of the growing phase. TfR was in close vicinity to HLA class I heavy and light chains on HUT-102B cells in both plateau and logarithmic phases, while it was not associated with HLA class I on the surface of JY cells. HLA class II molecules form a cluster with TfR on HUT-102B cells, while only a modest association was found on JY cells, and only in the logarithmic phase. The possible explanation of this distinct association and a two dimensional model of the antigen and receptor distributions are presented in this paper.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Immunology Letters. - 44 : 2-3 (1995), p. 203-208. -
További szerzők:Bene László (1963-) (biofizikus) Heiligen, Harry Rausch, Jeff Damjanovich Sándor (1936-2017) (biofizikus)
Pályázati támogatás:1492
OTKA
T6221
OTKA
T6163
OTKA
ETT 469
OTKA
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

8.

001-es BibID:BIBFORM046282
Első szerző:Nagy Péter (biofizikus)
Cím:Ion-channel activities regulate transmembrane signaling in thymocyte apoptosis and T-cell activation / Nagy Péter, Panyi György, Jenei Attila, Bene László, Gáspár Rezső, Matkó János, Damjanovich Sándor
Dátum:1995
ISSN:0165-2478
Megjegyzések:Several examples have shown that plasma membrane ion channels (e.g., Ca2+ and K+ channels) make an important contribution to lymphocyte activation or thymocyte apoptosis. Here we report on the importance of these ion channels in the sensitivity or resistance of lymphoid cells to extracellular ATP-induced apoptosis. Thymocytes of Balb/c mice responded to extracellular ATP (ATPex) sensitively, with an immediate increase in the intracellular calcium level and later with an increased membrane permeability to low MW markers. Mature (medullary) thymocytes showed a higher sensitivity than did cortical thymocytes. Three human lymphoma cell lines, including SUPT13, a cell line reported to be sensitive to TcR/CD3 activation-induced apoptosis, showed a high resistance to ATPex action. These observations suggest that maturation/differentiation state-dependent activity or disappearance of early ATP-receptor operated signaling systems (including ion channels) are critical for the cells in developing towards apoptosis. Using the patch-clamp technique we demonstrated that bretylium tosylate (a particular K(+)-channel blocker) known as inhibitor of T-lymphocyte proliferation also influences the single-channel properties of voltage-gated K+ channels through depressing whole-cell K+ currents. This finding is yet another example underlying the importance of K+ channel activity in T-lymphocyte proliferation.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Immunology Letters. - 44 : 2-3 (1995), p. 91-95. -
További szerzők:Panyi György (1966-) (biofizikus) Jenei Attila (1966-) (biofizikus) Bene László (1963-) (biofizikus) Gáspár Rezső (1921-2001) (fizikus) Matkó János (1952-) (biológus) Damjanovich Sándor (1936-2017) (biofizikus)
Pályázati támogatás:T14655
OTKA
F13335
OTKA
T6163
OTKA
T6221
OTKA
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

9.

001-es BibID:BIBFORM035604
Első szerző:Varga Zsuzsa (biokémikus, nephrológus)
Cím:Cell surface markers, inositol phosphate levels and membrane potential of lymphocytes from young and old human patients / Varga Zsuzsa, Bressani N., Zaia A. M., Bene László, Fülöp Tamás Jr., Leövey András, Fabris N., Damjanovich Sándor
Dátum:1990
ISSN:0165-2478
Megjegyzések:It is well known that most physiological functions change with aging, including the immune response. Data concerning the aging of lymphocyte subpopulations are conflicting. The antigen density of peripheral blood lymphocytes has been determined by fluorescently tagged OKT-3, OKT-4, OKT-8, OKT-11 and OKM1 monoclonal antibodies in a carefully selected aged (over 87 years) population, and compared to that of young subjects. A substantial difference was found in the percentage distribution of OKT8 and OKM1 subsets. The volume of lymphocytes of the elderly population was significantly less than that of the young. The effect of various monoclonal antibodies on phosphatidylinositol breakdown has also been studied. It was found that only OKT3, acting through the CD3 antigen receptor, was able to induce inositol phosphate formation in both young and elderly, although in the latter population this occurred at a lower level. Because the plasma membrane plays a regulatory role in this process, an important and sensitive functional parameter, the membrane potential, was also monitored and influenced by changing the extracellular K+ concentration. The lymphocytes of the elderly population responded less sensitively to changes in extracellular potassium concentration.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Immunology Letters. - 23 : 4 (1990), p. 275-280. -
További szerzők:Bressani, N. Zaia, A. M. Fülöp Tamás Jr Leövey András (1926-) (belgyógyász) Fabris, N. Damjanovich Sándor (1936-2017) (biofizikus) Bene László (1963-) (biofizikus)
Borító:
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