Találati lista | Tudóstér

001-es BibID:	BIBFORM004657
Első szerző:	Bene László (biofizikus)
Cím:	Detection of receptor clustering by flow cytometric fluorescence anisotropy measurements / Bene, L., Fulwyler, M. J., Damjanovich, S.
Dátum:	2000
Megjegyzések:	Perrin equation suggests an alternative way for the accurate energy transfer determination on a cell-by-cell basis by measuring polarized donor intensities in a conventional flow cytometer. METHODS: The relationship between energy transfer and fluorescence anisotropy of the donor was investigated by flow cytometric generation of Perrin-lifetime plots of fluorescent antibody-labeled MHC class I and class II molecules on the surface of living cells. The energy transfer reduced the fluorescence lifetime of the donor. RESULTS: Perrin plots have proven to be sensitive to the segmental mobility of the labeling dye and that of antibodies of different isotypes, and homo-transfer due to the multiple labeling of antibodies. A method demonstrating the feasibility of energy transfer determination by measuring anisotropy enhancement of the donor is presented. Flow cytometric histograms of the donor anisotropy and of the deduced energy transfer efficiency are shown, indicating clustering of MHC class I and class II molecules on the surface of human T lymphoblasts. In the Appendix, a method for the simultaneous determination of both energy transfer efficiency and donor fluorescence anisotropy, without need for G-factor measurement, is also presented. CONCLUSIONS: We demonstrate that energy transfer efficiency, i.e., proximity, between suitably selected donor and acceptor, and the rotational relaxation of the donor, i.e., donor mobility, can be simultaneously measured in a flow cytometer
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Adult analysis Antibodies beta 2-Microglobulin Cells diagnostic use Dyes Energy Transfer Flow Cytometry Fluorescence Fluorescence Polarization Fluorescent Dyes Histocompatibility Antigens Class I Histocompatibility Antigens Class II Human Hungary Immunoglobulin G immunology methods Receptors,Cell Surface Support,Non-U.S.Gov't T-Lymphocytes
Megjelenés:	Cytometry. - 40 : 4 (2000), p. 292-306. -
További szerzők:	Fulwyler, Mack J. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:	elektronikus változat DOI
Borító:
Saját polcon: