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1.

001-es BibID:BIBFORM106367
035-os BibID:(cikkazonosító)389 (scopus)85126609863 (wos)000775439000001
Első szerző:Bene László (biofizikus)
Cím:T-cell receptor is a threshold detector : sub- and supra-threshold stochastic resonance in TCR-MHC clusters on the cell surface / Bene László, Bagdány Miklós, Damjanovich László
Dátum:2022
ISSN:1099-4300
Megjegyzések:Stochastic resonance in clusters of major histocompatibility molecules is extended by a more detailed description of adaptive thresholding and by applying the notion of suprathreshold stochastic resonance as a stochastically quantizing encoder of transmembrane signaling downstream of major histocompatibility molecules and T-cell receptors on the side of presenting and recognizing cells, respectively. The adaptive nature of thresholding is partly explained by a mirroring of the noncognate?cognate dichotomy shown by the T-cell receptor structure and the kinetic-segregation model of the onset of T-cell receptor triggering. Membrane clusters of major histocompatibility molecules and T-cell receptors on their host cells are envisioned as places of the temporal encoding of downstream signals via the suprathreshold stochastic resonance process. The ways of optimization of molecular prostheses, such as chimeric antigen receptors against cancer in transmembrane signaling, are suggested in the framework of suprathreshold stochastic resonance. The analogy between Förster resonance energy transfer and suprathreshold stochastic resonance for information transfer is also discussed. The overlap integral for energy transfer parallels the mutual information transferred by suprathreshold stochastic resonance.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
noncognate-cognate discrimination
MHCI/MHCII
TCR
CD8/CD4
distributed signal detection
chimeric antigen receptor (CAR) optimization
mutual information
correlation
pattern recognition
Förster resonance energy transfer (FRET)
Megjelenés:Entropy. - 24 : 3 (2022), p. 1-21. -
További szerzők:Bagdány Miklós Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:1G3DBLR0TUDF-247
Egyéb
OSTRAT/810/213
Egyéb
TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
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2.

001-es BibID:BIBFORM099747
035-os BibID:(WOS)000753681400016 (Scopus)85119967213
Első szerző:Bene László (biofizikus)
Cím:Fluorescence Resonance Energy Transfer Gating by Elliptically Polarized Light on the Cell Surface / Bene László, Gogolák Péter, Ungvári Tamás, Bagdány Miklós, Rubovszky Bálint, Damjanovich László
Dátum:2021
Tárgyszavak:Természettudományok Fizikai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:ACS Photonics. - 8 (2021), p. 3548-3563. -
További szerzők:Gogolák Péter (1968-) (biológus, immunológus) Ungvári Tamás Bagdány Miklós Rubovszky Bálint (1975-) (élettanász) Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:1G3DBLR0TUDF-247
Egyéb
OSTRAT/810/213
Egyéb
TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
Sebészet Kutatócsoport
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3.

001-es BibID:BIBFORM099746
035-os BibID:(cikkazonosító)113519
Első szerző:Bene László (biofizikus)
Cím:Excitation polarization angle-resolved single-laser dual-polarization energy transfer on the cell surface / Bene László, Bagdány Miklós, Ungvári Tamás, Rubovszky Bálint, Damjanovich László
Dátum:2021
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal of Photochemistry & Photobiology, A: Chemistry. - 421 (2021), p. 1-12. -
További szerzők:Bagdány Miklós Ungvári Tamás Rubovszky Bálint (1975-) (élettanász) Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
Sebészet Kutatócsoport
1G3DBLR0TUDF-247
Egyéb
OSTRAT/810/213
Egyéb
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4.

001-es BibID:BIBFORM090881
035-os BibID:(cikkazonosító)113144
Első szerző:Bene László (biofizikus)
Cím:Information theoretic FRET calibration on the cell surface / Bene László, Bagdány Miklós, Ungvári Tamás, Damjanovich László
Dátum:2021
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal of Photochemistry & Photobiology, A: Chemistry. - 409 (2021), p. 1-13. -
További szerzők:Bagdány Miklós Ungvári Tamás Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
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5.

001-es BibID:BIBFORM082468
Első szerző:Bene László (biofizikus)
Cím:Adaptive threshold-stochastic resonance (AT-SR) in MHC clusters on the cell surface / László Bene, Miklós Bagdány, László Damjanovich
Dátum:2019
ISSN:0165-2478
Megjegyzések:Highly conserved 2D receptor clusters (membrane rafts) of immunological signaling molecules with MHCI and MHCII antigens as their cores have been observed in the past on the surface of T- and B-cell lines of lymphoid origin, as well as on cells from patients with colon tumor and Crohn's disease. Conservativity is related to the ever presence of MHCI molecules. Although they are suspected to play a role in maintaining these clusters and facilitating transmembrane signaling, their exact role has been left largely enigmatic. Here we are suggesting stochastic resonance (SR), or "noise-assisted signal detection", as a general organizing principle for transmembrane signaling events evoked by processes like immune recognition and cytokine binding taking place in these clusters. In the conceptual framework of SR, in immune recognition as a prototype of transmembrane signaling, the sea of self-peptide-MHC complexes around a nonself-peptide presenting MHC is conceived as a source of quickly fluctuating unspecific signal ("athermal noise") serving the extra energy for amplifying the weak sub-threshold specific signal of the nonself-peptide presenting MHC. This same noise is also utilized for a readjustment of the threshold ? and also the sensitivity and specificity ? of detection by a closed loop feedback control of the TcR-CD8 (CD4) proximity on the detecting T-cell. The weak sub threshold specific signal of nonself-peptide presenting MHC is amplified by the superposing unspecific signals of the neighboring self peptide-MHC complexes towards the T-cell receptor as the detector. Because in a successful detection event both self- and nonself-peptides are detected simultaneously, the principle of coincidence (or lock-in) detection is also realized. The ever presence of MHC islands gets a natural explanation as a source of extra power ? in a form of "athermal noise" ? needed for coincidence detection and frequency encoding the evoked downstream signals. The effect is quite general, because the actual type of molecules surrounding a chief signaling molecule ? like nonself-peptide holding MHC, interleukin-2 and -15 cytokine receptors (IL-2R/15R) ? as the fluctuating interaction energy sources is immaterial. The model applies also for other types of signaling, such as those evoked by cytokine binding. The phenomenon of SR can also be interpreted as sampling of a low frequency, specific signal with a high frequency unspecific signal, the "noise". Recipes for identifying other forms of SR in membrane clusters with biophysical tools are recommended.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Immunology Letters. - 217 (2019), p. 65-71. -
További szerzők:Bagdány Miklós Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
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6.

001-es BibID:BIBFORM075134
035-os BibID:(WoS)000444060600014 (Scopus)85052296237
Első szerző:Bene László (biofizikus)
Cím:Dual-Laser Tetra-Polarization FRET (4polFRET) for Site-Selective Control of Homo-FRET in Hetero-FRET Systems on the Cell Surface : the Homo-FRET Gate / Bene L., Bagdány M., Ungvári T., Damjanovich L.
Dátum:2018
ISSN:0003-2700
Megjegyzések:The effects of donor homo-Förster resonance energy transfer (homo-FRET) taking place in hetero-FRET systems is described in the context of hetero-FRET detection via donor and acceptor fluorescence anisotropies in cell surface receptor clusters. Donor homo-FRET can influence both the efficiency of detection as well as the magnitude of the detectable hetero-FRET. A 4-fold polarized FRET detection scheme-tetrapolarization FRET (4polFRET)-is proposed not only for discriminating the effects of homo-FRET from those of hetero-FRET, but also for correlating homo-associations of the donors and acceptors at different donor-acceptor distances, even beyond the critical Förster distance for hetero-FRET ( R0). The method is based on suppressing homo-FRET at the donor side with red-edge excitation. After the anisotropy effects of physical rotation and homo-FRET were separated by site-selective spectroscopy, the magnitude of the effect of homo-FRET on hetero-FRET has been estimated. It has been found significant, offering a new sensitive technique for detecting conformational dynamics via the homo-FRET mediated component of hetero-FRET, the "homo-FRET enhanced hetero-FRET" or "homo-FRET gate". The method is realizable in flow, as well as in image cytometry equipped with polarization detecting facility.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
four-fold fluorescence anisotropy correlations
dual-hetero-FRET
dual-homo-FRET
homo-FRET-sensitized hetero-FRET
donor fluorescence anisotropy
acceptor fluorescence anisotropy
Megjelenés:Analytical Chemistry. - 90 : 17 (2018), p. 10159-10170. -
További szerzők:Bagdány Miklós Ungvári Tamás Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
Sebészet Kutatócsoport
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7.

001-es BibID:BIBFORM075133
Első szerző:Bene László (biofizikus)
Cím:Checkpoint for helicity conservation in fluorescence at the nanoscale : energy and helicity transfer (hFRET) from a rotating donor dipole / László Bene, Miklós Bagdány, László Damjanovich
Dátum:2018
Megjegyzések:Orientation factor (?2) for FRET from a rotating donor dipole, transferred helicity of the donor field and torqueexerted on the acceptor by the donor have been investigated in the framework of classical electrodynamics. It isshown that for rotating dipole, ?2 is significantly higher as compared to linear dipole independently of theorientation distribution of the donor and acceptor and whether the static or the dynamic rotational regimes areused for averaging ?2. By this property of ?2, FRET serves as an example for a phenomenon where local fieldinterference may take place in a "natural" way for emitters possessing rotating dipoles in their excited states bynature. The overlapping spatial distributions for the helicity of donor local field, torque exerted on the acceptorby the donor and for the FRET orientational factor suggest that transfer of both energy and helicity take placepredominantly in the plane of rotation by keeping the original direction of helicity, i.e. in accordance with theconservation law for helicity.Orienting FRET has been proposed by engineering local field structure by using elliptically polarized light fordonor excitation or by using linearly polarized light coupled with electromagnetic modification of the donorenvironment. The phenomenon of increased ?2 can be exploited for checking helicity conservation for differentFRET donors without the need for polarized detection optics. Modulation of FRET with changing ellipticity of theexcited donor state might supply structural and dynamical information on the orientational distribution of dyeholdingmatrices even on the surface of living cells, e.g. on the level of cell surface receptor clusters.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Orientation factor for FRET (?2), Elliptically polarized light, Angular momentum, Torque of local field, Local field interference, Nanoparticle (quantum dot/QD Plasmonic)
Megjelenés:Biophysical Chemistry. - 239 (2018), p. 38-53. -
További szerzők:Bagdány Miklós Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
Sebészet Kutatócsoport
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8.

001-es BibID:BIBFORM065560
Első szerző:Bene László (biofizikus)
Cím:Depolarized FRET (depolFRET) on the cell surface : FRET control by photoselection / László Bene, Péter Gogolák, Tamás Ungvári, Miklós Bagdány, István Nagy, László Damjanovich
Dátum:2016
ISSN:0167-4889
Megjegyzések:Sensitivity of FRET in hetero- and homo-FRET systems on the photoselected orientation distribution of donors has been proven by using polarized and depolarized light for excitation. FRET as well as donor and acceptor anisotropies have been simultaneously measured in a dual emission-polarization scheme realized in a conventional flow cytometer by using single laser excitation and applying fluorophore-conjugated mAbs against the MHCI and MHCII cell surface receptors. Depolarization of the originally polarized light have been achieved by using crystal depolarizers based on Cornu's principle, a quarter-wave plate for circular polarization, and a parallel beam splitter acting as a diagonal-polarizer for dual-polarization excitation. Simultaneous analysis of intensity-based FRET efficiency and acceptor depolarization equivocally report that depolarization of light may increase FRET in an amount depending on the acceptor-to-donor concentration ratio. Acceptor depolarization turned to be more sensitive to FRET than donor hyper-polarization and even than intensity-based FRET efficiency. It can be used as a sensitive tool for monitoring changes in the dynamics of the donor-acceptor pairs. The basic observations of FRET enhancement and increased acceptor depolarization obtained for hetero-FRET are paralleled by analog observations of homo-FRET enhancements under depolarized excitation. In terms of the orientation factor for FRET, the FRET enhancements on depolarization in the condition of the macroscopically isotropic orientation distributions such as those of the cell surface bound fluorophores report on the presence of local orientation mismatches of the donor and acceptor preventing the optimal FRET in the polarized case, which may be eliminated by the excitation depolarization. A theory of fluorescence anisotropy for depolarized excitation is also presented.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Polarizationally structured light
Circularly polarized light
Diagonal-polarization
CORNU-depolarizer
Orientation factor for FRET
Homo-FRET
Megjelenés:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. - 1863 : 2 (2016), p. 322-334. -
További szerzők:Gogolák Péter (1968-) (biológus, immunológus) Ungvári Tamás Bagdány Miklós Nagy István (1957-) (villamosmérnök) Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
Sebészet Kutatócsoport
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9.

001-es BibID:BIBFORM069692
Első szerző:Ungvári Tamás
Cím:Perrin and Förster unified : dual-laser triple-polarization FRET (3polFRET) for interactions at the Förster-distance and beyond / Tamás Ungvári, Péter Gogolák, Miklós Bagdány, László Damjanovich, László Bene
Dátum:2016
ISSN:0167-4889
Megjegyzések:Dual laser flow cytometric energy transfer (FCET)--elaborated by Trón et al. in 1984--is an efficient and rapid way of measuring FRET on large cell populations. FRET efficiency and the donor and acceptor concentrations are determined from one donor and two acceptor signals. In this communication this method is extended towards the domain of receptor dynamics by the detection of polarized components of the three intensities. By enabling a complete description of the proximity and dynamics of FRET-systems, the new measuring scheme allows a more refined description of both the structure and dynamics of cell surface receptor clusters at the nano-scale and beyond. Associated donor fraction, limiting anisotropy and rotational correlation time of the donor, acceptor anisotropy and cell-by-cell estimation of the orientation factor for FRET (K2) are available in the steady state on a single FRET sample in a very rapid and statistically efficient way offered by flow cytometry. For a more sensitive detection of conformational changes the "polarized FRET indices"--quantities composed from FRET efficiency and anisotropies--are proposed. The method is illustrated by measurements on a FRET system with changing FRET-fraction and on a two donor-one acceptor-system, when the existence of receptor trimers are proven by the detection of "hetero-FRET induced homo-FRET relief", i.e. the diminishing of homo-FRET between the two donors in the presence of a donor quencher. The method also offers higher sensitivity for assessing conformational changes at the nano-scale, due to its capability for the simultaneous detection of changes of proximity and relative orientations of the FRET donor and acceptor. Although the method has been introduced in the context of FRET, it is more general: It can be used for monitoring triple-anisotropy correlations also in those cases when FRET actually does not occur, e.g. for interactions occuring beyond the Förster-distance R0. Interpretation of K2 has been extended.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Triple-anisotropy correlations
Donor anisotropy
Acceptor anisotropy
Orientation factor for FRET
Homo-FRET relief
FRET-fraction
Megjelenés:Biochimica et Biophysica Acta (BBA). Molecular Cell Research. - 1863 : 4 (2016), p. 703-716. -
További szerzők:Gogolák Péter (1968-) (biológus, immunológus) Bagdány Miklós Damjanovich László (1960-) (általános sebész) Bene László (1963-) (biofizikus)
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