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001-es BibID:	BIBFORM004645
035-os BibID:	(scopus)0033552675 (wos)000084290400003
Első szerző:	Damjanovich Sándor (biofizikus)
Cím:	Two-dimensional receptor patterns in the plasma membrane of cells : a critical evaluation of their identification, origin and information content / Damjanovich, S., Bene, L., Matko, J., Matyus, L., Krasznai, Z., Szabo, G., Pieri, C., Gaspar, R., Szollosi, J.
Dátum:	1999
Megjegyzések:	A concise review is presented on the nature, possible origin and functional significance of cell surface receptor patterns in the plasma membrane of lymphoid cells. A special emphasize has been laid on the available methodological approaches, their individual virtues and sources of errors. Fluorescence energy transfer is one of the oldest available means for studying non-randomized co-distribution patterns of cell surface receptors. A detailed and critical description is given on the generation of two-dimensional cell surface receptor patterns based on pair-wise energy transfer measurements. A second hierarchical-level of receptor clusters have been described by electron and scanning force microscopies after immuno-gold-labeling of distinct receptor kinds. The origin of these receptor islands at a nanometer scale and island groups at a higher hierarchical (mum) level, has been explained mostly by detergent insoluble glycolipid-enriched complexes known as rafts, or detergent insoluble glycolipids (DIGs). These rafts are the most-likely organizational forces behind at least some kind of receptor clustering [K. Simons et al., Nature 387 (1997) 569]. These models, which have great significance in trans-membrane signaling and intra-membrane and intracellular trafficking, are accentuating the necessity to revisit the Singer-Nicolson fluid mosaic membrane model and substitute the free protein diffusion with a restricted diffusion concept.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Biophysics Cells Diffusion Energy Transfer Fluorescence Hungary Microscopy
Megjelenés:	Biophysical Chemistry. - 82 : 2-3 (1999), p. 99-108. -
További szerzők:	Bene László (1963-) (biofizikus) Matkó János (1952-) (biológus) Mátyus László (1956-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) Szabó Gábor (1953-) (biofizikus) Pieri, Carlo Gáspár Rezső (1944-) (biofizikus) Szöllősi János (1953-) (biofizikus)
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001-es BibID:	BIBFORM004659
Első szerző:	Dzoljic, M.
Cím:	Ethanol and halothane differently modulate HLA class I and class II oligomerization : a new look at the mode of action of anesthetic agents through fluorescence spectroscopy / Dzoljic, M., Bene, L., Krasznai, Z., Damjanovich, S., Van Duijn, B.
Dátum:	2000
Megjegyzések:	The field of research considering the working mechanism of anesthetic agents is a complex one and the site or sites of action of general anesthetics are yet to be elucidated. Through the years, on the molecular level, the discussion has shifted from the lipid theories to the more specific interaction with the proteins responsible for the signal transduction. While this approach led to several models, they offer, at best, partial explanations for the observed phenomena. Anesthetic agents interact with many systems, of which the neuronal is best studied, leaving interaction with the immune defense system relatively unexplored. In this study we focus on the interaction of ethanol and halothane with the co-localization on the membrane of HLA I and II molecules. We show that ethanol tends to randomize the distribution of HLA I and II molecules, while halothane increases the clustering of HLA I proteins. The notion that anesthetics modulate cell function by disrupting clustering and thereby promoting a random distribution is a novel approach that may explain the general involvement of many systems during exposition to anesthetic drugs. In this study we show the disturbance of co-localization of molecules that may form a functional network. The relevance of this finding depends on the importance of these networks for extracellular and intracellular processes.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Anesthetics, Inhalation Antibodies, Monoclonal B-Lymphocytes Cell Line chemistry Comparative Study drug effects Energy Transfer Ethanol Fluorescence Halothane Histocompatibility Antigens Class I Histocompatibility Antigens Class II Human Macromolecular Systems methods pharmacology Research Signal Transduction Spectrometry, Fluorescence Support, Non-U.S.Gov't
Megjelenés:	Journal of Photochemistry and Photobiology. B, Biology. - 56 : 1 (2000), p. 48-52. -
További szerzők:	Bene László (1963-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Duijn, B., Van
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001-es BibID:	BIBFORM006042
035-os BibID:	PMID:8419464
Első szerző:	Márián Teréz (radiobiológus)
Cím:	Hypo-osmotic shock induces an osmolality-dependent permeabilization and structural changes in the membrane of carp sperm / Teréz Márián, Zoltán Krasznai, László Balkay, Margit Balázs, Miklós Emri, László Bene, Lajos Trón
Dátum:	1993
Megjegyzések:	We carried out spectrofluorimetric and flow cytometric measurements to investigate the effect of hypo-osmotic shock on cell membranes of common carp sperm. The time course of the permeability of the sperm cell membrane, as monitored by DNA-related propidium iodide (PI) fluorescence, was followed for 30 min after dilution of semen in hypo-osmotic environments of different ionic strengths. Spectrofluorimetric measurements indicated a continuous increase in the total PI emission intensity of a sperm suspension. Cell-by-cell flow cytometric measurements suggested that the permeability changes were of the all-or-none type. The permeabilized fraction of cells in the individual samples was time and osmolality dependent. The number and percentage of cells in which DNA was stained by PI increased gradually over time and reached a steady-state plateau value after 5-15 min. This equilibrium fraction of cells with a PI-permeable cytoplasmic membrane displayed an inverse relationship with the osmolality of the diluent, having a near 100% value for fresh water and distilled water. Dilution of sperm in hypo-osmotic medium brought about a fast decrease in the forward light-scattering signal on a short time scale compared to the pre-steady-state time of the permeabilization. With the addition of extracellular Ca2+ (1.8 mM), restoration of the light scattering signal was observed. Permeabilization of the membrane and restoration of light scattering were not coincident in time. We propose a two-dimensional reorganization of the lipid structure as the underlying mechanism of the latter process.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Animal Calcium Calcium Chloride Carps Cell Membrane Cell Membrane Permeability Cells Dna drug effects Flow Cytometry Fluorescence Hungary Light Male Osmolar Concentration Osmotic Pressure pharmacology physiology Propidium Semen Spectrometry,Fluorescence Spermatozoa Support,Non-U.S.Gov't ultrastructure Water egyetemen (Magyarországon) készült közlemény
Megjelenés:	The Journal of Histochemistry and Cytochemistry. - 41 : 2 (1993), p. 291-297. -
További szerzők:	Krasznai Zoltán (1950-) (biofizikus) Balkay László (1963-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Emri Miklós (1962-) (fizikus) Bene László (1963-) (biofizikus) Trón Lajos (1941-) (biofizikus)
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001-es BibID:	BIBFORM004884
Első szerző:	Rubovszky Bálint (élettanász)
Cím:	Detection of channel proximity by nanoparticle-assisted delaying of toxin binding : a combined patch-clamp and flow cytometric energy transfer study / Rubovszky, B., Hajdu, P., Krasznai, Z., Gaspar, R., Waldmann, T. A., Damjanovich, S., Bene, L.
Dátum:	2005
ISSN:	0175-7571
Megjegyzések:	Gold nanoparticles of 30 nm diameter bound to cell-surface receptor major histocompatibility complex glycoproteins (MHCI and MHCII), interleukin-2 receptor alpha subunit (IL-2Ralpha), very late antigen-4 (VLA-4) integrin, transferrin receptor, and the receptor-type protein tyrosin phosphatase CD45 are shown by the patch-clamp technique to selectively modulate binding characteristics of Pi(2) toxin, an efficient blocker of K(v)1.3 channels. After correlating the electrophysiological data with those on the underlying receptor clusters obtained by simultaneously conducted flow cytometric energy transfer measurements, the modulation was proved to be sensitive to the density and size of the receptor clusters, and to the locations of the receptors as well. Based on the observation that engagement of MHCII by a monoclonal antibody down-regulates channel current and based on the close nanometer-scale proximity of the MHCI and MHCII glycoproteins, an analogous experiment was carried out when gold nanoparticles bound to MHCI delayed down-regulation of the K(v)1.3 current initiated by ligation of MHCII. Localization of K(v)1.3 channels in the nanometer-scale vicinity of the MHC-containing lipid rafts is demonstrated for the first time. A method is proposed for detecting receptor-channel or receptor-receptor proximity by observing nanoparticle-induced increase in relaxation times following concentration jumps of ligands binding to channels or to receptors capable of regulating channel currents.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Antibodies Biophysics Cell Line chemistry drug effects Energy Transfer Flow Cytometry Glycoproteins Gold Human Humans Interleukin-2 Interleukin-2 Receptor alpha Subunit Ion Channel Gating Kv1.3 Potassium Channel Ligands Major Histocompatibility Complex methods Nanotubes Particle Size Patch-Clamp Techniques pharmacokinetics physiology Potassium Potassium Channels Potassium Channels,Voltage-Gated Protein Binding Research Scorpion Venoms Support T-Lymphocytes
Megjelenés:	European Biophysics Journal. - 34 : 2 (2005), p. 127-143. -
További szerzők:	Hajdu Péter (1975-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Waldmann, Thomas A. Damjanovich Sándor (1936-2017) (biofizikus) Bene László (1963-) (biofizikus)
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