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1.

001-es BibID:BIBFORM034559
Első szerző:Damjanovich Sándor (biofizikus)
Cím:An introduction to the working principles of the flow cytometry / Damjanovich, S., Pieri, C., Bene, L., Jenei, A., Gaspar, R.
Dátum:1997
ISSN:978-3540625636
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
egyetemen (Magyarországon) készült közlemény
Megjelenés:Signal Transduction - Single Cell Techniques / eds. Wiltink, Anneke; Van Duijn, Bert. - p. 343-350
További szerzők:Pieri, Carlo Bene László (1963-) (biofizikus) Jenei Attila (1966-) (biofizikus) Gáspár Rezső (1944-) (biofizikus)
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2.

001-es BibID:BIBFORM034558
Első szerző:Damjanovich Sándor (biofizikus)
Cím:Flow cytometric membrane potential measurements / Damjanovich, S., Pieri, C., Bene, L., Jenei, A., Gaspar, R.
Dátum:1997
ISSN:978-3540625636
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
Signal Transduction
egyetemen (Magyarországon) készült közlemény
Megjelenés:Signal Transduction - Single Cell Techniques / eds. Wiltink, Anneke; Van Duijn, Bert. - p. 348-357
További szerzők:Pieri, Carlo Bene László (1963-) (biofizikus) Jenei Attila (1966-) (biofizikus) Gáspár Rezső (1944-) (biofizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM004645
035-os BibID:(scopus)0033552675 (wos)000084290400003
Első szerző:Damjanovich Sándor (biofizikus)
Cím:Two-dimensional receptor patterns in the plasma membrane of cells : a critical evaluation of their identification, origin and information content / Damjanovich, S., Bene, L., Matko, J., Matyus, L., Krasznai, Z., Szabo, G., Pieri, C., Gaspar, R., Szollosi, J.
Dátum:1999
Megjegyzések:A concise review is presented on the nature, possible origin and functional significance of cell surface receptor patterns in the plasma membrane of lymphoid cells. A special emphasize has been laid on the available methodological approaches, their individual virtues and sources of errors. Fluorescence energy transfer is one of the oldest available means for studying non-randomized co-distribution patterns of cell surface receptors. A detailed and critical description is given on the generation of two-dimensional cell surface receptor patterns based on pair-wise energy transfer measurements. A second hierarchical-level of receptor clusters have been described by electron and scanning force microscopies after immuno-gold-labeling of distinct receptor kinds. The origin of these receptor islands at a nanometer scale and island groups at a higher hierarchical (mum) level, has been explained mostly by detergent insoluble glycolipid-enriched complexes known as rafts, or detergent insoluble glycolipids (DIGs). These rafts are the most-likely organizational forces behind at least some kind of receptor clustering [K. Simons et al., Nature 387 (1997) 569]. These models, which have great significance in trans-membrane signaling and intra-membrane and intracellular trafficking, are accentuating the necessity to revisit the Singer-Nicolson fluid mosaic membrane model and substitute the free protein diffusion with a restricted diffusion concept.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Biophysics
Cells
Diffusion
Energy Transfer
Fluorescence
Hungary
Microscopy
Megjelenés:Biophysical Chemistry. - 82 : 2-3 (1999), p. 99-108. -
További szerzők:Bene László (1963-) (biofizikus) Matkó János (1952-) (biológus) Mátyus László (1956-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) Szabó Gábor (1953-) (biofizikus) Pieri, Carlo Gáspár Rezső (1944-) (biofizikus) Szöllősi János (1953-) (biofizikus)
Internet cím:DOI
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4.

001-es BibID:BIBFORM046293
Első szerző:Gáspár Rezső (biofizikus)
Cím:Effect of acetylcholine on the electrophysiology and proliferative response of human lymphocytes / Gaspar R., Varga Z., Bene L., Marcheselli F., Pieri C., Damjanovich S.
Dátum:1996
ISSN:0006-291X
Megjegyzések:Using the patch-clamp technique, we determined that 1-15 mM extracellular acetylcholine reduced whole-cell n-type K+ currents in human peripheral blood lymphocytes and accelerated their inactivation. The percentage increase in K+ channel inactivation rate and the degree of drug induced block were independent of membrane potential. In flow cytometric membrane potential measurements with the oxonol dye similar doses of acetylcholine depolarized the lymphocyte population. Both acetylcholine induced K+ channel block and depolarization fully developed within 2 minutes. The depolarizing and K+ channel blocking effects of acetylcholine are in concert. [3H]thymidine incorporation experiments proved that the proliferative response of PHA stimulated peripheral blood lymphocytes was decreased by increasing concentrations of acetylcholine in the 1-50 mM range.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biochemical And Biophysical Research Communications. - 226 : 2 (1996), p. 303-308. -
További szerzők:Varga Zoltán (1969-) (biofizikus, szakfordító) Bene László (1963-) (biofizikus) Marcheselli, Fiorella Pieri, Carlo Damjanovich Sándor (1936-2017) (biofizikus)
Pályázati támogatás:T14655
OTKA
T13335
OTKA
6221
OTKA
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DOI
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5.

001-es BibID:BIBFORM004946
Első szerző:Jenei Attila (biofizikus)
Cím:HLA class I and II antigens are partially co-clustered in the plasma membrane of human lymphoblastoid cells / Jenei, A., Varga, S., Bene, L., Matyus, L., Bodnar, A., Bacso, Z., Pieri, C., Gaspar, R., Farkas, T., Damjanovich, S.
Dátum:1997
ISSN:0027-8424
Megjegyzések:Major histocompatibility complex (MHC) class II molecules displayed clustered patterns at the surfaces of T (HUT-102B2) and B (JY) lymphoma cells characterized by interreceptor distances in the micrometer range as detected by scanning force microscopy of immunogold-labeled antigens. Electron microscopy revealed that a fraction of the MHC class II molecules was also heteroclustered with MHC class I antigens at the same hierarchical level as described by the scanning force microscopy data, after specifically and sequentially labeling the antigens with 30- and 15-nm immunogold beads. On JY cells the estimated fraction of co-clustered HLA II was 0.61, whereas that of the HLA I was 0.24. Clusterization of the antigens was detected by the deviation of their spatial distribution from the Poissonian distribution representing the random case. Fluorescence resonance energy transfer measurements also confirmed partial co-clustering of the HLA class I and II molecules at another hierarchical level characterized by the 2- to 10-nm Forster distance range and providing fine details of the molecular organization of receptors. The larger-scale topological organization of the MHC class I and II antigens may reflect underlying membrane lipid domains and may fulfill significant functions in cell-to-cell contacts and signal transduction.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Cell Membrane
Energy Transfer
Fluorescence
Histocompatibility Antigens Class I
Histocompatibility Antigens Class II
Human
Hungary
immunology
Lymphocytes
Major Histocompatibility Complex
Microscopy
Microscopy, Electron
Signal Transduction
ultrastructure
Megjelenés:Proceedings of the National Academy of Sciences of the United States of America. - 94 : 14 (1997), p. 7269-7274. -
További szerzők:Varga Sándor (1943-) (biofizikus) Bene László (1963-) (biofizikus) Mátyus László (1956-) (biofizikus) Dóczy-Bodnár Andrea (1970-) (biofizikus) Bacsó Zsolt (1963-) (biofizikus) Pieri, Carlo Gáspár Rezső (1944-) (biofizikus) Farkas Tibor (kutató) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
elektronikus változat
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6.

001-es BibID:BIBFORM006046
Első szerző:Mátyus László (biofizikus)
Cím:Voltage gating of Ca2(+)-activated potassium channels in human lymphocytes / Matyus, L., Pieri, C., Recchioni, R., Moroni, F., Bene, L., Tron, L., Damjanovich, S.
Dátum:1990
Megjegyzések:The effect of membrane potential on Ca2+ activated K+ channels was studied on human peripheral lymphocytes. Membrane potential was monitored using bisoxonol and flow cytometry. 1 mM Ca2+ in the presence of 2 microM ionomycin depolarized the control cell population, while 100 microM Ca2+ caused hyperpolarization. However 1 mM Ca2+ had a hyperpolarizing effect on previously partially depolarized cells. Potassium channel blockers did not influence the depolarization, while they inhibited the hyperpolarization. Based on the experimental evidence a voltage gating of Ca2+ activated K+ channels is suggested.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Biophysics
Calcium
drug effects
Flow Cytometry
Human
Hungary
In Vitro
Ion Channel Gating
Ionomycin
Leukocytes,Mononuclear
Lymphocytes
Membrane Potentials
pharmacology
physiology
Potassium
Potassium Channel Blockers
Potassium Channels
Support,Non-U.S.Gov't
Megjelenés:Biochemical and Biophysical Research Communications. - 171 : 1 (1990), p. 325-329. -
További szerzők:Pieri, Carlo Recchioni, Rina Moroni, Fausto Bene László (1963-) (biofizikus) Trón Lajos (1941-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
DOI
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7.

001-es BibID:BIBFORM004635
Első szerző:Péter Mózes (orvos, neuroradiológus) ifj.
Cím:Pandinus imperator scorpion venom blocks voltage-gated K+ channels in human lymphocytes / Peter, M. Jr., Varga, Z., Panyi, G., Bene, L., Damjanovich, S., Pieri, C., Possani, L. D., Gaspar, R.
Dátum:1998
ISSN:006-291X
Megjegyzések:Using the patch-clamp technique, we determined that Pandinus imperator scorpion venom blocked whole-cell n-type K+ currents in human peripheral blood lymphocytes in a dose-dependent manner with Kd = 0.02 microgram/ml. K+ channel block was instantaneous and removable by washing with venom-free extracellular solution. The venom-induced block was independent of membrane potential. The venom did not influence activation and inactivation kinetics of the K+ channels, however, accelerated recovery from inactivation. Purified peptides Pi1, Pi2, and Pi3 from the P. imperator venom powerfully blocked Kv1.3 channels in human lymphocytes with Kd values of 9.7 nM, 50 pM, and 0.5 nM, respectively. Flow cytometric membrane potential measurements with the oxonol dye showed that Pi2, the most effective peptide toxin of the P. imperator venom, depolarizes human lymphocytes in accordance with its K+ channel blocking effect.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Barbiturates
blood
drug effects
Electrophysiology
Flow Cytometry
Fluorescent Dyes
Human
isolation and purification
Isoxazoles
Kinetics
Lymphocytes
Membrane Potentials
metabolism
Patch-Clamp Techniques
pharmacology
physiology
Potassium
Potassium Channel Blockers
Potassium Channels
Protein Binding
Scorpion Venoms
Support, Non-U.S.Gov't
Support, U.S.Gov't, P.H.S.
Toxins
Megjelenés:Biochemical and Biophysical Research Communications. - 242 : 3 (1998), p. 621-625. -
További szerzők:Varga Zoltán (1969-) (biofizikus, szakfordító) Panyi György (1966-) (biofizikus) Bene László (1963-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Pieri, Carlo Possani, Lourival Domingos Gáspár Rezső (1944-) (biofizikus)
Internet cím:DOI
elektronikus változat
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8.

001-es BibID:BIBFORM046306
Első szerző:Varga Zoltán (biofizikus, szakfordító)
Cím:The Effect of Juglone on the Membrane Potential and Whole-Cell K+Currents of Human Lymphocytes / Varga Z., Bene L., Pieri C., Damjanovich S., Gaspar R.
Dátum:1996
ISSN:0006-291X
Megjegyzések:Using flow cytometric membrane potential measurements with the oxonol dye, we determined that 5.7-57 microM juglone depolarizes human lymphocytes in a dose dependent manner. The depolarizing effect of juglone was verified by patch-clamp. Juglone decreased whole-cell n-type K+ currents in human peripheral blood lymphocytes and accelerated inactivation; however, it did not influence the kinetics of activation of the K+ conductance. The percentage increase in K+ channel inactivation rate and the degree of drug induced block was independent of membrane potential, K+ channel block by juglone fully developed within 4 minutes and was not removable by washing with drug free extracellular solution. Blocking of n-type K+ channels by juglone is in concert with its depolarizing effect on human lymphocytes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biochemical And Biophysical Research Communications. - 218 : 3 (1996), p. 828-832. -
További szerzők:Bene László (1963-) (biofizikus) Pieri, Carlo Damjanovich Sándor (1936-2017) (biofizikus) Gáspár Rezső (1944-) (biofizikus)
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DOI
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9.

001-es BibID:BIBFORM033278
035-os BibID:PMID:8588941 WOS:A1995TF18900001
Első szerző:Vereb György (biofizikus, orvos)
Cím:Plasma-membrane-bound macromolecules are dynamically aggregated to form non-random codistribution patterns of selected functional elements. Do pattern recognition processes govern antigen presentation and intercellular interactions? / György Vereb, László Matyus, László Bene, György Panyi, Zsolt Bacsó, Margit Balázs, János Matkó, János Szöllősi, Rezső Gáspár, Sándor Damjanovich, Robert E. Dale, Carlo Pieri, Marcel Ameloot
Dátum:1995
Megjegyzések:Molecular recognition processes between cell surface elements are discussed with special reference to cell surface pattern formation of membrane-bound integral proteins. The existence, as detected by flow cytometric resonance energy transfer (Appendix), and significance of cell surface patterns involving the interleukin-2 receptor, the T-cell receptor-CD3 system, the intercellular adhesion molecule ICAM-1, and the major histocompatibility complex class I and class II molecules in the plasma membrane of lymphocytes are described. The modulation of antigen presentation by transmembrane potential changes is discussed, and a general role of transmembrane potential changes, and therefore of ion channel activities, adduced as one of the major regulatory mechanisms of cell-cell communication. A general role in the mediation and regulation of intercellular interactions is suggested for cell-surface macromolecular patterns. The dynamic pattern of protein and lipid molecules in the plasma membrane is generated by the genetic code, but has a remarkable flexibility and may be one of the major instruments of accommodation and recognition processes at the cellular level.
Tárgyszavak:Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban
cell surface
molecular pattern
energy transfer
fluorescence
flow cytometry
transmembrane potential
MHC
antigen presentation
intercellular communication
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of Molecular Recognition. - 8 : 4 (1995), p. 237-246. -
További szerzők:Mátyus László (1956-) (biofizikus) Bene László (1963-) (biofizikus) Panyi György (1966-) (biofizikus) Bacsó Zsolt (1963-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Matkó János (1952-) (biológus) Szöllősi János (1953-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Dale, Robert E. Pieri, Carlo Ameloot, Marcel
Internet cím:DOI
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