CCL

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1.

001-es BibID:BIBFORM046298
Első szerző:Goda Katalin (biofizikus)
Cím:Reversal of Multidrug Resistance by Valinomycin is Overcome by CCCP / Goda K., Krasznai Z., Gaspar R., Lankelma J., Westerhoff H. V., Damjanovich S., Szabo G.
Dátum:1996
ISSN:0006-291X
Megjegyzések:Reversal of P-glycoprotein-mediated multidrug resistance by valinomycin is overcome by the proton ionophore, CCCP. This effect, a complete suppression of the 5- to 10-fold valinomycin-induced reversal ("re-reversal"), exhibits a sharp extracellular potassium concentration ([K+(0)]) dependence. It is observed at [K+(0)] > 2-4 mM and not at [K+(0)] greater than or equal to 2 mM, in the case of the fluorescent substrates rhodamine 123 and daunorubicin. The fact that "re-reversal" is detected only for the combination of CCCP with valinomycin raises the possibility that a direct interaction between these ionophores may explain the phenomenon. We show spectroscopic evidence of such an interaction, with a [K+(0)]-dependence similar to that of the "re-reversal." These data suggest that the reversal of P-glycoprotein activity by valinomycin can be compromised by anionic compounds such as CCCP due to complex formation. More generally, molecular interactions involving P-glycoprotein substrates or reversing agents may significantly affect drug accumulation in multidrug resistant cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biochemical And Biophysical Research Communications. - 219 : 2 (1996), p. 306-310. -
További szerzők:Krasznai Zoltán (1950-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Lankelma, Jan Westerhoff, Hans V. Damjanovich Sándor (1936-2017) (biofizikus) Szabó Gábor (1953-) (biofizikus)
Pályázati támogatás:T14655
OTKA
17592
OTKA
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM004839
035-os BibID:(WOS)000220105600025 (scopus)1342345242
Első szerző:Nagy Henrietta
Cím:Distinct groups of multidrug resistance modulating agents are distinguished by competition of P-glycoprotein-specific antibodies / Nagy, H., Goda, K., Fenyvesi, F., Bacso, Z., Szilasi, M., Kappelmayer, J., Lustyik, G., Cianfriglia, M., Szabo, G.
Dátum:2004
Megjegyzések:P-glycoprotein (Pgp) is one of the ABC transporters responsible for the multidrug resistance of cancer cells. The conformational changes of Pgp that occur in the presence of substrates/modulators or ATP depletion are accompanied by the up-shift of UIC2 monoclonal antibody (mAb) binding. In the case of cyclosporin A, vinblastine or valinomycin, this up-shift was found to be concomitant with the near-complete suppression of labeling with other mAbs specific for Pgp epitopes overlapping with UIC2, while pre-treatment with verapamil or Tween 80 brings about a modest suppression. Here we have extended these observations to 44 Pgp interacting agents, and found that only 8 fall into the cyclosporin-like category, inducing a conformational state characterized by the complete UIC2 dominance. The rest of the drugs either did not affect antibody competition or had a modest effect. Thus, Pgp substrates/modulators can be classified into distinct modalities based on the conformational change they elicit.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Adenosine
Adenosine Triphosphatases
Animals
Anti-Bacterial Agents
Antibodies
Antibodies,Monoclonal
Antineoplastic Agents
Binding,Competitive
Biophysics
Calcium
Calcium Channel Blockers
Cells
Cyclosporine
Detergents
Drug Resistance,Multiple
Drug Resistance,Neoplasm
Epitopes
Flow Cytometry
Fluorescein
Fluoresceins
genetics
Humans
Hungary
immunology
Ivermectin
metabolism
Mice
Nih 3T3 Cells
P-Glycoprotein
pharmacology
physiology
Research
Substrate Specificity
Support
Valinomycin
Verapamil
Vinblastine
egyetemen (Magyarországon) készült közlemény
Megjelenés:Biochemical and Biophysical Research Communications. - 315 : 4 (2004), p. 942-949. -
További szerzők:Goda Katalin (1969-) (biofizikus) Fenyvesi Ferenc (1977-) (gyógyszerész, gyógyszertechnológus) Bacsó Zsolt (1963-) (biofizikus) Szilasi Mária (1953-) (tüdőgyógyász, klinikai immunológus, allergológus, belgyógyász) Kappelmayer János (1960-) (laboratóriumi szakorvos) Lustyik György Cianfriglia, Maurizio Szabó Gábor (1953-) (biofizikus)
Internet cím:elektronikus változat
DOI
Borító:

3.

001-es BibID:BIBFORM024103
035-os BibID:(scopus)0025708015 (wos)A1990DJ97600054
Első szerző:Szabó Gábor (biofizikus)
Cím:Disassembly of chromatin into approximately equal to 50 kb units by detergent / G. Szabó, F. Boldog, N. Wikonkál
Dátum:1990
Megjegyzések:Nuclei isolated from higher eukaryotic cell lines were directly analyzed by field inversion gel electrophoresis. Brief incubation of nuclei with ionic detergents yielded a single band between 50-100 kb. The apparent fragment size decreased to approximately equal to 50 kb after proteinase digestion. The latter treatment alone induced less regular, less than or equal to 50 kb fragmentation. DNA extracted from detergent and proteinase-treated nuclei also appeared in a band of about 40 kb. Embedding into agarose plugs did not protect nuclei, as opposed to cells, from detergent-induced fragmentation. The phenomenon is strikingly analogous to the double-strand DNA cleavage reactions mediated by topoisomerase II. Our data are compatible with any of the following interpretations: 1.) regularly spaced protein bridges, probably involving topoisomerase II, maintain or control continuity of chromosomal DNA in certain states of higher eukaryotic cells. 2.) The DNA might become accessible to a putative endonuclease at regularly spaced sites upon detergent treatment of isolated nuclei.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Chromatin
Megjelenés:Biochemical and Biophysical Research Communications. - 169 : 2 (1990), p. 706-712. -
További szerzők:Boldog F. Wikonkál Norbert (1968-) (bőrgyógyász)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
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4.

001-es BibID:BIBFORM024101
035-os BibID:(scopus)0024457009 (wos)A1989AM56600050
Első szerző:Szabó Gábor (biofizikus)
Cím:Efficient Retroviral Transfer of A Mouse C-Myc Construct Into Hl6O / Gábor Szabo Jr., Gösta Winberg, Marie Henriksson, George Klein, János Sumegi
Dátum:1989
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
mouse
Megjelenés:Biochemical and Biophysical Research Communications. - 163 : 1 (1989), p. 321-327. -
További szerzők:Winberg, Gösta Henriksson, Marie Klein, George Sümegi János
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
Borító:

5.

001-es BibID:BIBFORM004654
035-os BibID:(scopus)0344199417 (wos)000083351400015
Első szerző:Varga Tamás (biológus)
Cím:Single-strand breaks in agarose-embedded chromatin of nonapoptotic cells / Varga, T., Szilagyi, I., Szabo, G.
Dátum:1999
Megjegyzések:Loop-size chromatin fragmentation frequently observed upon apoptotic cell death is thought to be initiated by ss nicks. Here we show that the agarose-embedded, deproteinized chromatin of normal, non-apoptotic murine and human cells, as well as yeast protoplasts, falls apart to 50-300 kb ss fragments upon heat denaturation, as revealed by urea-TAE field-inversion agarose gel electrophoresis resolving ss and ds fragments alike. These data were in line with S1digestion experiments. The nicks (gaps) observed are best explained either by enzymatic cleavages occurring upon cell lysis instantaneously or by preexisting discontinuities becoming manifest upon heat denaturation. These discontinuities go unnoticed in the usual nondenaturaing circumstances but seem to be inevitably present in any DNA preparation. The loop-size ds DNA fragmentation in apoptosis may be based on these pre-existing or "ready-to-go" (upon cell lysis) ss discontinuities of the normal cellular chromatin
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Animals
Apoptosis
Aspergillus Nuclease S1
Biophysics
Cell Line
Cells
chemistry
Chromatin
Dna
DNA Damage
DNA Fragmentation
DNA,Single-Stranded
Electrophoresis, Agar Gel
Electrophoresis, Gel, Pulsed-Field
Heat
Human
Humans
Hungary
Mice
Nucleic Acid Denaturation
Research
Saccharomyces cerevisiae
Sepharose
Support
ultrastructure
Urea
Megjelenés:Biochemical and Biophysical Research Communications. - 264 : 2 (1999), p. 388-394. -
További szerzők:Szilágyi Ildikó (orvos) Szabó Gábor (1953-) (biofizikus)
Internet cím:DOI
elektronikus változat
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