CCL

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1.

001-es BibID:BIBFORM024098
Első szerző:Sejersen, Thomas
Cím:Similarities and differences in the regulation of N-myc and c-myc genes in murine embryonal carcinoma cells / Sejersen T., Rahm M., Szabo G., Ingvarsson S., Sumegi J.
Dátum:1987
Megjegyzések:c-myc and N-myc are closely related genes coding for putative DNA-binding proteins. The protein products of both genes have been implicated in the regulation of growth of normal and neoplastic cells. We compared the regulation of N-myc and c-myc expression under different growth conditions as well as in vitro differentiation of the murine EC lines F9 and PCC7. N-myc and c-myc expression was found to be regulated by distinct mechanisms, although similarities exist. Differences were found both at the transcriptional and at the post-transcriptional level. The two myc genes were regulated by mainly posttranscriptional mechanisms, but in PCC7 cells nuclear run-on assays indicated that c-myc was repressed at the level of transcription. N-myc and c-myc expression was negatively regulated at a post-transcriptional level in F9 and PCC7 cells during differentiation to visceral endoderm and nerve-like tissue, respectively. Serum stimulation of F9 cells for 4 h induced a sevenfold increase in c-myc transcripts but no significant elevation of N-myc transcripts. Mitogenic stimulation with insulin and transferrin also induced a marked elevation of c-myc but not of N-myc mRNA. In addition, the N-myc and c-myc genes differed in F9 cells with respect to (i) the kinetics of expression following induction of differentiation, c-myc undergoing quicker changes than N-myc; (ii) the response to cycloheximide inhibition of protein synthesis, indicating that c-myc but not N-myc is down-regulated by a short-lived protein; and (iii) the half-lives of the transcripts, estimated to be approximately 40 min of c-myc and 130 min for N-myc
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animal
Carcinoma
carcinoma cell
Cell Differentiation
Cell Line
Cells
Comparative Study
cycloheximide
differentiation
DNA-Binding Proteins
Endoderm
Gene Expression Regulation
genetics
In Vitro
Insulin
Kinetics
Mice
Neoplasm Proteins
Neurons
nonhuman
protein synthesis
Proteins
proto oncogene
Proto-Oncogene Proteins
Proto-Oncogene Proteins c-myc
Proto-Oncogenes
rna
Support,Non-U.S.Gov't
Teratoma
Transferrin
Tumor Cells,Cultured
Megjelenés:Experimental Cell Research. - 172 : 2 (1987), p. 304-317. -
További szerzők:Rahm, Magnus Szabó Gábor (1953-) (biofizikus) Ingvarsson, Sigurdur Sümegi János
Internet cím:elektronikus változat
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DOI
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2.

001-es BibID:BIBFORM024111
035-os BibID:(scopus)0028972652 (wos)A1995TH62300008
Első szerző:Szabó Gábor (biofizikus)
Cím:50-Kb Chromatin Fragmentation in the Absence of Apoptosis / Szabo G.
Dátum:1995
Megjegyzések:Treatment with ionic detergents of nuclei isolated from various continuously growing cell lines generally yields chromatin samples of high viscosity, Extensive treatment with nuclease-free proteinase K or pronase solubilized the viscous lysates with > 90% of the DNA migrating at similar to 50 kb. Freshly prepared human peripheral blood T cells also yield a substantial fraction of their DNA in an similar to 50- to 100-kb band, The cleavage sites may coincide with a class of DNase I-hypersensitive regions, since digestion of chromatin by DNase I at similar to 10 U/ml, without protease, also yields fragments of preferentially similar to 50-kb size, Occasionally, the oligonucleosomal ladder was also detected together with high molecular weight degradation products, Remarkably, all of these fragmentation patterns were seen in healthy, resting or proliferating cells, i.e., in the absence of apoptosis, Tritiated thymidine incorporation could be readily detected in the similar to 50-kb DNA fragments, The effect of an apoptotic intracellular milieu on the integrity of isolated chromatin is apparently imitated by the extensive protease treatment used in our DNA isolation protocol.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Apoptosis
blood
Cell Line
Cells
Chromatin
Detergents
Dna
Human
Molecular Weight
Thymidine
Viscosity
Megjelenés:Experimental Cell Research. - 221 : 2 (1995), p. 320-325. -
Internet cím:elektronikus változat
Intézményi repozitóriumban (DEA) tárolt változat
DOI
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3.

001-es BibID:BIBFORM024104
Első szerző:Szabó Gábor (biofizikus)
Cím:Inositol derivatives down-regulate c-myc inducing growth arrest without differentiation / G. Szabó, L. Székely, M. Schablik, G. Klein, J. Sümegi, G. Szabó
Dátum:1991
Megjegyzések:Cyclitol derivatives that are structurally related to myo-inositol induce growth arrest without differentiation in human promyelocytic leukemia (HL60) cells. An early effect is the rapid down-regulation of c-myc mRNA levels. This was observed also in several mouse and human lines carrying either normal or rearranged myc. The mRNA levels of a constitutive mouse myc construct transfected into HL60 were not affected at the same time. Uridine and thymidine incorporation were significantly decreased by the cyclitol treatment. These effects partly resemble those of certain differentiation inducers and those of hexachlorcyclohexane, another myo-inositol analogue. This new group of agents offers a novel approach to studying control mechanisms involving c-myc.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
differentiation
Megjelenés:Experimental Cell Research. - 193 : 2 (1991), p. 420-424. -
További szerzők:Székely László Schablik Marcella Klein, George Sümegi János Szabó G. (biológus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:BIBFORM024100
Első szerző:Szabó Gábor (biofizikus)
Cím:Independent variations of myc amplification, inducibility, maturation, and proliferation states in HL60 / Szabo G., Bunce C., Brown G., Klein G., Sumegi J.
Dátum:1988
Megjegyzések:We have investigated the possible relationship between c-myc gene activity and other variable traits in HL60. In a panel of variant lines, a good correlation was observed between myc gene copy number and the level of myc mRNA. There was no correlation between myc amplification or expression and the resistance of the lines to induction of terminal neutrophilic or monocytic differentiation. Therefore, myc mRNA level does not appear to determine the ability of the variant HL60 lines to respond to inducers of differentiation. Flow cytometric analyses of the expression of a differentiation antigen (AGF 4.36) revealed stable negative and positive subpopulations in growing HL60. myc amplification, expression, and inducibility were identical in these subpopulations, suggesting that variation of these traits in HL60 sublines and variants is not due to maturation state differences. myc gene copy number was also identical in transferrin receptor positive (proliferating) and negative (resting) populations. These data contradict the notion that myc amplification has been important in determining the in vitro biological properties of HL60
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Cell Culture
Cell Division
cell growth
cell strain hl 60
controlled study
Dimethyl Sulfoxide
Dna
Flow Cytometry
Fluorescent Antibody Technique
Gene Amplification
Gene Expression Regulation
gene induction
Human
human cell
In Vitro
Leukemia,Myelocytic,Acute
Nucleic Acid Hybridization
oncogene myc
phorbol 13 acetate 12 myristate
priority journal
Proteins
Proto-Oncogene Proteins
Proto-Oncogene Proteins c-myc
Proto-Oncogenes
RNA,Messenger
Support, Non-U.S.Gov't
Support, U.S.Gov't,P.H.S.
Tumor Cells, Cultured
Megjelenés:Experimental Cell Research. - 175 : 2 (1988), p. 334-343. -
További szerzők:Bunce, Chris Brown, Geoffrey Klein, George Sümegi János
Internet cím:elektronikus változat
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5.

001-es BibID:BIBFORM006002
035-os BibID:(scopus)0023112835 (wos)A1987G382700017
Első szerző:Szabó Gábor (biofizikus)
Cím:Overall changes in chromatin sensitivity to DNase I during differentiation / Gábor Szabó, Sandor Damjanovich, János Sümegi, George Klein
Dátum:1987
Megjegyzések:The DNase I sensitivity of total chromatin was studied in fixed cells and nuclei isolated from proliferating and terminally differentiated cells, by measuring the incorporation of labelled nucleotides into DNase-sensitive sites, and electrophoresis of DNA isolated from DNase-treated nuclei. The unfixed nuclei were sensitive to digestion at around 10 micrograms/ml, the fixed cells at 30 ng/ml DNase I concentration. Proliferating Rauscher leukemia cells were more digestible than normal spleen cells. The DNase I sensitivity of the human HL60 leukemia line decreased upon DMSO-induced differentiation but still exceeded the digestibility of nuclei from normal human peripheral blood. A novel flow-cytometric technique was developed to study DNase sensitivity at the cell level. It confirmed the relative resistance of differentiated cells to DNase I and ruled out the possibility that this could be due to an altered distribution of cell cycle phases. The overall DNase I sensitivity of chromatin was compared with the sensitivity of the c-myc gene and the myc-associated hypersensitive sites. The latter sites were detected at 1 microgram/ml DNase I in HL60 nuclei. They disappeared partially upon DMSO-induced differentiation. At 10 micrograms/ml, myc was degraded in both growing and differentiating HL60, but not in HPB cells. These data suggest that a progressive condensation of the chromatin occurs during terminal differentiation which gradually involves specific genes that need to be inactivated.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Animal
blood
Cell Cycle
Cell Differentiation
Cell Division
Cell Line
Chromatin
cytology
Deoxyribonuclease I
Dna
Flow Cytometry
Human
Leukemia,Erythroblastic,Acute
Leukemia,Experimental
metabolism
Mice
Mice,Inbred BALB C
pathology
Spleen
Support,Non-U.S.Gov't
Support,U.S.Gov't,P.H.S.
Translation,Genetic
ultrastructure
Megjelenés:Experimental Cell Research. - 169 : 1 (1987), p. 158-168. -
További szerzők:Damjanovich Sándor (1936-2017) (biofizikus) Sümegi János Klein, George
Internet cím:elektronikus változat
DOI
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