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001-es BibID:	BIBFORM024098
Első szerző:	Sejersen, Thomas
Cím:	Similarities and differences in the regulation of N-myc and c-myc genes in murine embryonal carcinoma cells / Sejersen T., Rahm M., Szabo G., Ingvarsson S., Sumegi J.
Dátum:	1987
Megjegyzések:	c-myc and N-myc are closely related genes coding for putative DNA-binding proteins. The protein products of both genes have been implicated in the regulation of growth of normal and neoplastic cells. We compared the regulation of N-myc and c-myc expression under different growth conditions as well as in vitro differentiation of the murine EC lines F9 and PCC7. N-myc and c-myc expression was found to be regulated by distinct mechanisms, although similarities exist. Differences were found both at the transcriptional and at the post-transcriptional level. The two myc genes were regulated by mainly posttranscriptional mechanisms, but in PCC7 cells nuclear run-on assays indicated that c-myc was repressed at the level of transcription. N-myc and c-myc expression was negatively regulated at a post-transcriptional level in F9 and PCC7 cells during differentiation to visceral endoderm and nerve-like tissue, respectively. Serum stimulation of F9 cells for 4 h induced a sevenfold increase in c-myc transcripts but no significant elevation of N-myc transcripts. Mitogenic stimulation with insulin and transferrin also induced a marked elevation of c-myc but not of N-myc mRNA. In addition, the N-myc and c-myc genes differed in F9 cells with respect to (i) the kinetics of expression following induction of differentiation, c-myc undergoing quicker changes than N-myc; (ii) the response to cycloheximide inhibition of protein synthesis, indicating that c-myc but not N-myc is down-regulated by a short-lived protein; and (iii) the half-lives of the transcripts, estimated to be approximately 40 min of c-myc and 130 min for N-myc
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Animal Carcinoma carcinoma cell Cell Differentiation Cell Line Cells Comparative Study cycloheximide differentiation DNA-Binding Proteins Endoderm Gene Expression Regulation genetics In Vitro Insulin Kinetics Mice Neoplasm Proteins Neurons nonhuman protein synthesis Proteins proto oncogene Proto-Oncogene Proteins Proto-Oncogene Proteins c-myc Proto-Oncogenes rna Support,Non-U.S.Gov't Teratoma Transferrin Tumor Cells,Cultured
Megjelenés:	Experimental Cell Research. - 172 : 2 (1987), p. 304-317. -
További szerzők:	Rahm, Magnus Szabó Gábor (1953-) (biofizikus) Ingvarsson, Sigurdur Sümegi János
Internet cím:	elektronikus változat Intézményi repozitóriumban (DEA) tárolt változat DOI
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