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001-es BibID:	BIBFORM046864
Első szerző:	Fülöp Tamás (népegészségügyi szakember, egészségfejlesztő)
Cím:	Age-dependent changes in transmembrane signalling : identification of G proteins in human lymphocytes and polymorphonuclear leukocytes / Fulop Tamás, Barabas György, Varga Zsuzsa, József Csongor, Csabina Sándor, Szucs Sándor, Seres Ildikó, Szikszay Edit, Jeney Zsolt, Penyige András
Dátum:	1993
ISSN:	0898-6568
Megjegyzések:	In human neutrophils (PMNLs) we found that in the elderly IP3 formation was significantly decreased compared to that of young subjects. For FMLP receptor binding affinity and number no measurable differences occurred upon ageing, studying both the low or the high affinity receptors. The amount of ADP-ribosylated G proteins, catalysed by pertussis toxin (PT) or cholera toxin (CT), was significantly increased in PMNLs of the elderly. In lymphocytes, the PT-catalysed ADP ribosylation of G proteins was also increased with ageing, while the CT-catalysed ribosylation was decreased. The autoradiogram of [32P]ADP-ribosylated proteins by CT in lymphocytes of young individuals showed a major polypeptide of 40,000 M(r). In contrast, in lymphocytes of the elderly, the major polypeptide was 45,000 M(r). In PMNLs, CT labelled quite strongly the 45,000 M(r) band, mainly in the elderly. When PT was used, no age-related pattern changes could be demonstrated, while differences could be observed between the two types of cells. The use of antiserum P680 (G alpha common) showed no age-related pattern changes, while the intensity of the labelled proteins varies with age and cell type. The antiserum U46 (Go alpha) could identify in lymphocytes of young subjects two polypeptides 68,000 and 41,000 M(r). The prominent polypeptide in lymphocytes of the elderly was the 70,000 M(r) and no other polypeptides could be recognized. In PMNLs of young subjects the U46 and serum identified a range of species. In PMNLs of the elderly all these bands were weakly labelled. The present data indicate changes in the pattern and the quantity of G proteins in lymphocytes and PMNLs of elderly subjects.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Cellular Signalling. - 5 : 5 (1993), p. 593-603. -
További szerzők:	Barabás György (1933-) (sejtbiológus, molekuláris genetikus) Varga Zsuzsa (1951-) (biokémikus, nephrológus) József Csongor Csabina Sándor Szűcs Sándor (1958-) (biokémikus, vegyész) Seres Ildikó (1954-) (biokémikus) Szikszay Edit Jeney Zsolt Penyige András (1954-) (molekuláris genetikus)
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001-es BibID:	BIBFORM046287
Első szerző:	Panyi György (biofizikus)
Cím:	Peripheral blood lymphocytes display reduced K+ channel activity in aged humans / Panyi G., Berecki G., Gaspar R., Seres I., Fulop T., Damjanovich S.
Dátum:	1994
ISSN:	0006-291X
Megjegyzések:	Steady-state parameters of whole-cell K+ current have been determined in human peripheral blood lymphocytes of young (20-50 y.) and elderly (> 90 y.) volunteers by patch-clamp. The magnitude and voltage dependence of the K+ conductance were similar in both lymphocyte populations. The midpoint of steady-state inactivation was -53.3 +/- 2.3 mV for lymphocyte population of young individuals and -65.0 +/- 3.0 mV for that of elderly, showing a significant shift to hyperpolarized potentials. The peak of the steady-state open probability of the K+ channels was decreased and shifted to depolarized potentials by approx. 12.5 mV for lymphocytes of elderly donors. It is suggested that the observed differences in the K+ current parameters may be at least partly responsible for the impaired responsiveness of elderly lymphocytes to proliferative stimuli.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Biochemical and Biophysical Research Communications. - 199 : 2 (1994), p. 519-524. -
További szerzők:	Berecki Géza Gáspár Rezső (1944-) (biofizikus) Seres Ildikó (1954-) (biokémikus) Fülöp Tamás (1928-) (népegészségügyi szakember, egészségfejlesztő) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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