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001-es BibID:	BIBFORM049592
035-os BibID:	PMID:23870824
Első szerző:	Kis-Tóth Katalin (immunológus)
Cím:	Monocyte-derived dendritic cell subpopulations use different types of matrix metalloproteinases inhibited by GM6001 / Katalin Kis-Toth, Ildiko Bacskai, Peter Gogolak, Anett Mazlo, Istvan Szatmari, Eva Rajnavolgyi
Dátum:	2013
ISSN:	0171-2985
Megjegyzések:	Matrix metalloproteinases (MMPs) are endopeptidases with the potential to cleave extracellular matrix, support tissue renewal and regulate cell migration. Functional activities of MMPs are regulated by tissue inhibitors of MMPs (TIMPs) and disruption of the MMP-TIMP balance has pathological consequences. Here we studied the expression and secretion of MMPs and TIMPs in CD1a(-) and CD1a(+) monocyte-derived dendritic cell (DC) subpopulations. Our results showed that monocytes express TIMPs but lack MMPs, whereas upon differentiation to moDCs and in response to activation signals the expression of MMPs is increased and that of TIMPs is decreased. MMP-9 is expressed dominantly in the CD1a(-) subpopulation, while MMP-12 is preferentially expressed in CD1a(+) cells. Experiments performed with the synthetic MMP inhibitor GM6001 revealed that this drug efficiently inhibits the migration of moDCs through inactivation of MMPs. We conclude that modulation of MMP activity by GM6001 emerges as a novel approach to manipulate DC migration under inflammatory conditions. (C) 2013 Elsevier GmbH. All rights reserved.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban CD1a Dendritic cell Immunotherapy Matrix metalloproteinase Tissue inhibitor of matrix Metalloproteinase
Megjelenés:	Immunobiology. - 218 : 11 (2013), p. 1361-1369. -
További szerzők:	Bacskai Ildikó (1985-) (immunológus) Gogolák Péter (1968-) (biológus, immunológus) Türk-Mázló Anett (1989-) (molekuláris biológus) Szatmári István (1971-) (biológus) Rajnavölgyi Éva (1950-) (immunológus)
Pályázati támogatás:	TÁMOP-4.2.2.A-11/1/KONV-2012-0023-"VÉD-ELEM" TÁMOP NK 101538 OTKA
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001-es BibID:	BIBFORM020764
Első szerző:	Kis-Tóth Katalin (immunológus)
Cím:	Voltage-Gated Sodium Channel Nav1.7 Maintains the Membrane Potential and Regulates the Activation and Chemokine-Induced Migration of a Monocyte-Derived Dendritic Cell Subset / Katalin Kis-Toth, Peter Hajdu, Ildiko Bacskai, Orsolya Szilagyi, Ferenc Papp, Attila Szanto, Edit Posta, Peter Gogolak, Gyorgy Panyi, Eva Rajnavolgyi
Dátum:	2011
Megjegyzések:	Expression of CD1a protein defines a human dendritic cell (DC) subset with unique functional activities. We aimed to study the expression of the Nav1.7 sodium channel and the functional consequences of its activity in CD1a(-) and CD1a(+) DC. Single-cell electrophysiology (patch-clamp) and quantitative PCR experiments performed on sorted CD1a(-) and CD1a(+) immature DC (IDC) showed that the frequency of cells expressing Na(+) current, current density, and the relative expression of the SCN9A gene encoding Nav1.7 were significantly higher in CD1a(+) cells than in their CD1a(-) counterparts. The activity of Nav1.7 results in a depolarized resting membrane potential (-8.7 +/- 1.5 mV) in CD1a(+) IDC as compared with CD1a(-) cells lacking Nav1.7 (-47 +/- 6.2 mV). Stimulation of DC by inflammatory signals or by increased intracellular Ca(2+) levels resulted in reduced Nav1.7 expression. Silencing of the SCN9A gene shifted the membrane potential to a hyperpolarizing direction in CD1a(+) IDC, resulting in decreased cell migration, whereas pharmacological inhibition of Nav1.7 by tetrodotoxin sensitized the cells for activation signals. Fine-tuning of IDC functions by a voltage-gated sodium channel emerges as a new regulatory mechanism modulating the migration and cytokine responses of these DC subsets
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban ACTIVATION article Cells Electrophysiology Human Hungary immunology Sodium Tetrodotoxin
Megjelenés:	The Journal of Immunology. - 187 : 3 (2011), p. 1273-1280. -
További szerzők:	Hajdu Péter (1975-) (biofizikus) Bacskai Ildikó (1985-) (immunológus) Szilágyi Orsolya (1985-) (molekuláris biológus, biokémikus) Papp Ferenc (1979-) (biofizikus) Szántó Attila (1976-) (orvos, biokémikus) Feketéné Posta Edit (1986-) (reumatológus) Gogolák Péter (1968-) (biológus, immunológus) Panyi György (1966-) (biofizikus) Rajnavölgyi Éva (1950-) (immunológus)
Pályázati támogatás:	TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Molekuláris immunológia TÁMOP-4.2.2-08/1-2008-0015 TÁMOP
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001-es BibID:	BIBFORM037289
Első szerző:	Szabó Attila (molekuláris biológus, immunológus, filozófus)
Cím:	Temporally designed treatment of melanoma cells by ATRA and polyI:C results in enhanced chemokine and IFNb secretion controlled differently by TLR3 and MDA5 / Szabo Attila, Osman Rolah M., Bacskai Ildiko, Kumar Brahma V., Agod Zsofia, Lanyi Arpad, Gogolak Peter, Rajnavolgyi Eva
Dátum:	2012
ISSN:	0960-8931
Megjegyzések:	In the last three decades, the incidence of melanoma has increased worldwide and no effective treatment modalities have been developed yet. All-trans retinoic acid (ATRA) and polyinosinic:polycytidylic acid (polyI:C) are strong inducers of toll-like receptor 3 (TLR3) and MDA5 expression, and polyI:C-induced TLR3 and MDA5 signaling specifically causes cell death in melanoma cells in vitro. We addressed the question of whether ATRA pretreatment could enhance the efficacy of polyI:C and, if so, would ATRA have any additional effects on this process. We found that the combined treatment of human melanoma cells with ATRA and polyI:C strongly increased the expression of TLR3 and MDA5 in both WM35 and WM983A cells associated with significantly higher mRNA and secreted levels of interferon b (IFNb), CXCL1, CXCL8/IL-8, CXCL9, and CXCL10 than cells treated with either ATRA or polyI:C. Silencing of MDA5 by siRNA moderately affected IFNb secretion, whereas TLR3 knockdown interfered with both CXCL chemokine and IFNb production. Furthermore, the supernatants of ATRA + polyI:C-activated cultures increased the migration of both human monocyte-derived macrophages and CD1a + dendritic cells significantly as compared with the supernatants of cells treated with either ATRA or polyI:C, and this effect occurred in a TLR3-dependent manner. In conclusion, consecutive treatment with ATRA and polyI:C results in strong, TLR3/MDA5-mediated chemokine and IFN responses in cultured human melanoma cells, which triggers a functional migratory response in professional antigen-presenting cells. This novel mode of concomitant activation may represent a more efficient treatment option for future melanoma therapy.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Molekuláris Medicina
Megjelenés:	Melanoma Research 22 : 5 (2012), p. 351-361. -
További szerzők:	Osman, Rolah M. Bacskai Ildikó (1985-) (immunológus) Kumar, Brahma V. Agod Zsófia Lányi Árpád (1962-) (biológus, immunológus) Gogolák Péter (1968-) (biológus, immunológus) Rajnavölgyi Éva (1950-) (immunológus)
Pályázati támogatás:	TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Jelátviteli kapcsolatok ős- és dendritikus sejt altípusokban NK101538 OTKA
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