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001-es BibID:BIBFORM020092
Első szerző:Görögh Tibor
Cím:Transcriptional repression of the human galactocerebrosidase gene in squamous cell carcinomas of the larynx / Tibor Görögh, Heinrich Rudert, Burkard M. Lippert, Stefan Gottschlich, Steffen Maune, Klaus Heidorn, Jan Maass, Markus Hoffmann, Jens E. Meyer, Immo O. Rathcke, Benedikt J. Folz, Tibor Hortobagyi, Jochen A. Werner
Dátum:1999
ISSN:0020-7136
Megjegyzések:Alterations of gene expression in squamous cell carcinoma (SCC) cell lines derived from the larynx and keratinocytes derived from adjacent normal mucosa of the larynx have been studied using the mRNA differential display technique. Lane-to-lane comparison of reverse transcribed mRNA showed a strong repression of a 148 bp fragment in SCC cells. The fragment was reamplified and cloned. Sequencing revealed a 99.3% homology with a region in exon 17 of the human galactocerebrosidase (GALC) gene. Northern blot analysis confirmed the differential expression of this gene in both carcinoma cell lines and laryngeal SCC biopsies in contrast with corresponding normal mucosa. To provide further evidence for the differential expression rate, both types of cells were transiently transfected with a 152 bp (-176 to -24) high regulatory promoter element of the 5' flanking region of the GALC gene. Results of 3 independent transfection experiments indicated a 16-fold repression of the GALC gene expression in SCC cells compared with benign keratinocytes. However, neither mutation nor other alterations of the promoter sequence were detected. Expression of the GALC gene is thus greatly affected in SCCs of the larynx.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:International Journal of Cancer. - 83 : 6 (1999), p. 750-754. -
További szerzők:Rudert, Heinrich Lippert, Burkard M. Gottschlich, Stefan Maune, Steffen Heidorn, Klaus Maass, Jan Hoffmann, Markus Meyer, Jens E. Rathcke, Immo O. Folz, Benedikt J. Hortobágyi Tibor (1965-) (patológus) Werner, Jochen A.
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DOI
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001-es BibID:BIBFORM020088
Első szerző:Hortobágyi Tibor (patológus)
Cím:A novel brain trauma model in the mouse : effects of dexamethasone treatment / T. Hortobágyi, S. Hortobágyi, C. Görlach, T. Harkany, Z. Benyó, T. Görögh, W. Nagel, M. Wahl
Dátum:2000
ISSN:0031-6768
Megjegyzések:We describe a novel methodological approach for inducing cold lesion in the mouse as a model of human cortical contusion trauma. To validate its reproducibility and reliability, dexamethasone (Dxm) was repeatedly applied to demonstrate possible antioedematous drug effects. Following the induction of anaesthesia with halothane, the dura was exposed via trephination. Using a micromanipulator a pre-cooled (-78 degrees C) copper cylinder, 3 mm in diameter, was pressed down to a depth of 1 mm onto the dura for 30 s under microscopic control. The body temperature was held constant at 37 degrees C throughout the procedure. Blood pressure (BP), measured by a modified photosensor-monitored tail-cuff method, and acid-base status were not significantly different when analysed before and after cold lesion and prior to sacrifice. However, there was a marginal mixed respiratory and metabolic acidosis. The antioedematous action of Dxm was studied in four standard pre-and post-treatment paradigms: 2x0.5 mg/kg (II), 2x12.5 mg/kg (III) and 4x6.25 mg/kg (IV: 3x pre-, 1x post-treatment: V: 1x pre-, 3x post-treatment). Physiological saline injections served as controls. High doses of Dxm (III-V) significantly attenuated the cold-lesion-induced loss of body mass. Dxm treatment also resulted in a reduction of brain water content (III; P<0.05), and brain swelling (IV; P<0.05) in the lesioned hemisphere, relative to controls. In conclusion, we have characterized a novel cold lesion model in the mouse to mimic traumatic brain injury and the beneficial effect of Dxm treatment on the extent of brain oedema.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Pflügers Archiv. - 441 : 2-3 (2000), p. 409-415. -
További szerzők:Hortobágyi Szabolcs Görlach, Christoph Harkány Tibor Benyó Zoltán Görögh Tibor Nagel, W. Wahl, Michael
Internet cím:DOI
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