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001-es BibID:BIBFORM033103
035-os BibID:PMID:19597833
Első szerző:Csomor Péter (biotechnológus)
Cím:Restriction analysis of otosclerosis-associated CD46 splicing variants / Csomor Péter, Szalmás Anita, Kónya József, Sziklai István, Karosi Tamás
Dátum:2010
ISSN:0937-4477
Megjegyzések:Otosclerosis is a primary bone remodeling disorder of the human otic capsule and is associated with persistent measles virus infection. The human cellular receptor of measles virus is the membrane cofactor protein (MCP, CD46), which has 14 well-described splicing variants. Unique CD46 expression pattern of the otic capsule and the stapes footplate may determine the susceptibility for persistent measles virus infection. A total of 51 surgically removed ankylotic stapes footplates were analyzed by histopathological and molecular biological methods, respectively. Nucleic acids were extracted. Measles virus sequences were detected by nucleoprotein RNA-specific reverse transcriptase polymerase chain reaction (RT-PCR). Alternatively spliced RNA of CD46 isoforms was amplified by RT-PCR; cDNA amplimers were separated by SDS poly-acrylamide gel electrophoresis and were purified from the gel. Complementary DNA of CD46 isoforms was restricted by endonuclease enzymes having CD46-specific recognition sites. The presence of viral RNA was associated exclusively with the histopathological diagnosis of otosclerosis; the stapes specimens with negative measles virus belonged to non-otosclerotic stapes fixations. All specimens (N = 51) were characterized by the consecutive expression of five CD46 variants (c, d, e, f and one shorter unidentified isoform). Histologically confirmed ostosclerotic specimens (N = 21) were characterized by increased expression levels of variant "f" and the unknown isoform. Increased expression levels of these isoforms and special CD46 expression pattern of the human otic capsule might produce modified or pathological intracellular signalization that could create the possibility of persistent measles virus infection.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
CD46
Otosclerosis
Measles Virus
RT-PCR
Restriction analysis
egyetemen (Magyarországon) készült közlemény
Megjelenés:European Archives of Oto-Rhino-Laryngology. - 267 : 2 (2010), p. 219-226. -
További szerzők:Szalmás Anita (1978-) (biológus, mikrobiológus, klinikai mikrobiológus) Kónya József (1964-) (szakorvos, klinikai mikrobiológus) Sziklai István (1954-) (fül-orr-gégész) Karosi Tamás (1979-) (fül-orr-gégész)
Pályázati támogatás:OTKA PD75371
OTKA
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM013837
Első szerző:Karosi Tamás (fül-orr-gégész)
Cím:Osteoprotegerin expression and sensitivity in otosclerosis with different histological activity / Karosi Tamás, Csomor Péter, Szalmás Anita, Kónya József, Petkó Mihály, Sziklai István
Dátum:2011
ISSN:0937-4477
Megjegyzések:Otosclerosis is a complex bone dystrophy of the human otic capsule leading to conductive and sensorineural hearing loss. Since otosclerosis may, at least in part, be considered as an autoimmune-inXammatory disease, disturbed balance of TNF-alpha and osteoprotegerin (OPG) expression has been implicated in the pathological bone remodeling. It has been supposed that active otosclerosis is characterized by decreased or missing local OPG production with invariable OPG sensitivity of the otosclerotic foci. Ankylotic stapes footplates (n = 41) removed by stapedectomy were processed to histological examination, OPGspeciWc RT-PCR, tissue culturing and alkaline-phosphatase (AP) activity assessment, respectively. OPG concentration of serum specimens (n = 41) was measured by ELISA. Cortical bone fragments harvested from the external ear canal were used as negative controls of otosclerosis. Among 41 ankylotic stapes footplates, 22 active and 19 inactive otosclerosis cases were histologically diagnosed. OPG expression was signiWcantly lower (p < 0.001) in active otosclerosis compared to inactive cases. Osteoclast cultures originated from active otosclerotic foci showed a considerable susceptibility against external OPG dosage, which resulted in a signiWcant decrease of AP activity (p < 0.001). In contrast, OPG serum levels were in the normal range (5-100 ng/ml) indicating a non-systemic bone resorption. In conclusion, secondary decreased local OPG production might play an important role in the pathogenesis of otosclerotic bone remodeling disorder. As to previous and current results, decreased OPG sensitivity of lesion-forming cells should be excluded. These observations may indicate the potential role of recombinant OPG treatment in early stages of otosclerosis.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
otosclerosis
Megjelenés:European Archives Of Oto-Rhino-Laryngology. - 268 : 3 (2011), p. 357-365. -
További szerzők:Csomor Péter (1984-) (biotechnológus) Szalmás Anita (1978-) (biológus, mikrobiológus, klinikai mikrobiológus) Kónya József (1964-) (szakorvos, klinikai mikrobiológus) Petkó Mihály (1943-) (orvos, neurobiológus) Sziklai István (1954-) (fül-orr-gégész)
Pályázati támogatás:PD 75371
OTKA
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

3.

001-es BibID:BIBFORM001624
Első szerző:Karosi Tamás (fül-orr-gégész)
Cím:Expression of measles virus receptors in otosclerotic, non-otosclerotic and in normal stapes footplates / Karosi Tamás, Jókay István, Kónya József, Petkó Mihály, Z. Szabó László, Sziklai István
Dátum:2007
Megjegyzések:Otosclerosis is a bone remodeling disorder of complex etiology. Persistent measles virus infection of the otic capsule could increase the expression level of measles virus receptors (CD46) on the osteoclasts and endothelial cells of the otosclerotic foci. Presence of measles virus RNA was demonstrated in the footplates of histologically diagnosed otosclerotic patients by RT-PCR; however, no reports were available about the CD46 expression pattern and level in otosclerosis. Nucleic acid was extracted from stapes footplates of clinically otosclerotic patients (N = 116). Genomic RNA of measles virus was amplified by RT-PCR. Amplification results were correlated with postoperative histologic and CD46 specific immunhistologic findings. Among 116 stapes fixation cases, 87 otosclerotic stapes contained measles virus RNA. Histology for virus negative stapes (N = 29) represented degenerative disorders with heterogeneous histopathology. Active otosclerosis was featured by increased numbers of osteoclasts showing strong CD46 expression. In virus negative, non-otosclerotic stapes fixation and in normal stapes footplates weak CD46 immunoreaction was demonstrated on the osteocytes and fibroblasts. In otosclerosis, it is reasonable to assume that measles virus increases the expression level of its own cellular receptor. Furthermore, intensive CD46 reaction could relate to active virus replication and continuous receptor internalisation. Otosclerosis is a disease of disturbed osteoid turnover due to persistent measles virus infection and special CD46 receptor pattern of the otic capsule.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
footplate
measles virus
Megjelenés:European Archives of Oto-Rhino-Laryngology 264 : 6 (2007), p. 607-613. -
További szerzők:Jókay István (1954-) (fül-orr-gégész) Kónya József (1964-) (szakorvos, klinikai mikrobiológus) Petkó Mihály (1943-) (orvos, neurobiológus) Z. Szabó László Sziklai István (1954-) (fül-orr-gégész)
Internet cím:elektronikus változat
DOI
elektronikus változat
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