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001-es BibID:BIBFORM030355
035-os BibID:WOS:A1992HG21400007 PMID:1372849
Első szerző:Nánási Péter Pál (élettanász)
Cím:Repetitive electrical activity of the muscle membrane induced in chloride-free medium / P. P. Nanasi, M. Danko
Dátum:1992
ISSN:0305-1870
Megjegyzések:1. Superficial fibres of frog skeletal muscle were electrically stimulated in Ringer solution where the chloride content had been replaced by various weakly permeant anions. Changes of the membrane potential were recorded at three different time scales. The complex response was initiated by a volley of fast repetitive action potentials (10-20 ms cycle length) superimposed on the ascending phase of a transient depolarization to - 35 mV. The transient depolarization was followed by a membrane potential oscillation (0.3-0.6 s cycle length). The parameters of the volley and membrane potential oscillation were not greatly affected by the substituent anion. 2. The transient depolarization was fully abolished by tetrodotoxin (3-mu-mol/L), but left unaffected by nifedipine (5-mu-mol/L), or by the replacement of extracellular Ca for Ni or Co. Tetraethylammonium (20-40 mmol/L) increased the duration and amplitude of the transient depolarization. The shape of transient depolarization was uniform in a given fibre, in spite of its marked variability under different experimental conditions. 3. Single outward current pulses applied in chloride-free solution containing tetraethylammonium (20-40 mmol/L) evoked prolonged depolarizations to positive membrane potentials accompanied by increases in the specific membrane conductance. This slow response, which was also frequently observed in the absence of TEA, was mediated by Ca ions, as it was insensitive to tetrodotoxin (3-mu-mol/L) but abolished by nifedipine (10-mu-mol/L). 4. Two populations of the muscle fibres were observed during the slow response. Some fibres repolarized completely, while others failed to produce complete repolarization but formed a plateau between - 20 and - 30 mV, lasting for several minutes. When the external K concentration was abruptly increased to 5 or 10 mmol/L during the plateau of the slow response, repolarization and increase in membrane conductance were observed. 5. In muscle fibres, having osmotically disrupted T-system, the duration of transient depolarization was in the range of minutes, in contrast to the range of seconds observed in intact fibres. The volley was preserved in glycerol treated fibres, however, the baseline of discharges was close to the resting potential and the rate of depolarization of the baseline was significantly less in glycerol treated than in intact fibres. 6. These results are consistent with the existence of a second stable membrane potential level in skeletal muscle between - 40 and - 30 mV. The depolarization and repolarization during the membrane potential oscillation and transient depolarization can be regarded as a partial or full transition, respectively, between these two stable membrane potential levels, possibly due to the conductance changes of the inward rectifier K channels. It seems likely that the various types of repetitive activity are also linked to these conductance changes indicating that they are endogenous properties of the skeletal muscle membrane.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Clinical and Experimental Pharmacology and Physiology. - 19 : 2 (1992), p. 127-136. -
További szerzők:Dankó Miklós
Pályázati támogatás:1453
OTKA
Internet cím:DOI
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2.

001-es BibID:BIBFORM030338
035-os BibID:(WoS)A1996UY14300002 (Scopus)0029798762 (PMID)8842570
Első szerző:Nánási Péter Pál (élettanász)
Cím:Electrical restitution in diseased human ventricular myocardium / Péter P. Nanasi, Andras Varro, Csaba Pankucsi, Péter Homolay, Timothy K. Knilans, László Kovacs, Gyula J. Papp, David A. Lathrop
Dátum:1996
ISSN:0144-5979
Megjegyzések:Action potential configuration and electrical restitution were studied in diseased human ventricular muscle by comparing the characteristics of hypertrophic (HYP) and dilated (DIL) human ventricular preparations. Conventional microelectrode techniques were used to evaluate action potentials evoked at increasingly longer diastolic intervals, The steady-state action potential duration (APD(90)) was significantly longer in DIL than in HYP preparations (393+/-5 ms, n=4 and 296+/-11 ms, n=4, respectively; P<0.001, mean+/-SEM). In the dilated preparations studied at long diastolic intervals, the initial period of rapid repolarization (phase 1) was absent, and the rate of final repolarization (phase 3) was reduced. Electrical restitution relations in these preparations were fitted as the sum of two exponentials, The time constant of the fast component was significantly longer in DIL than in HYP preparations (242+/-9 ms and 121+/-4 ms, respectively; P<0.001). No difference was observed in the time constants for the slow component of restitution in the two groups. Electrical restitution was also studied in single human ventricular myocytes by using patch clamp techniques. The initial 600 ms period of restitution was fitted in these cells to a monoexponential function. The time constant for this period of the restitution relation was significantly longer, while the estimated amplitude of this early rising phase was significantly lower in human cells obtained from DIL hearts than the respective parameters obtained in the healthy canine and guinea pig cells also examined, The observed changes in the restitution kinetics of the dilated human heart are, likely, the consequence of alterations in the ionic currents that underlie the cardiac action potential.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
egyetemen (Magyarországon) készült közlemény
Megjelenés:Clinical Physiology. - 16 : 4 (1996), p. 339-351. -
További szerzők:Varró András (1954-) (farmakológus, klinikai farmakológus) Pankucsi Csaba (farmakológus) Homolay Péter (1947-1999) (mellkassebész, szívsebész) Knilans, Timothy K. Kovács László (1939-) (élettanász) Papp Gy. Julius (Szeged) Lathrop, David A.
Internet cím:DOI
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