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001-es BibID:BIBFORM071357
Első szerző:Nagy Gábor (laboratóriumi szakorvos, laboratóriumi hematológus és immunológus)
Cím:The role of automated microscopes and computer aided pattern recognition in autoantibody detection by indirect / G. Nagy, I. Csípő, J. Kappelmayer, P. Antal-Szalmás
Dátum:2016
Megjegyzések:The role of automated microscopes and computer aided pattern recognition in autoantibody detection by indirectimmunofluorescence assaysG. Nagy, I. Csípő, J. Kappelmayer, P. Antal-SzalmásDepartment of Laboratory Medicine, Faculty of Medicine, University of Debrecen, Debrecen, HungaryIndirect immunofluorescene assays (IFA) are versatile and sensitive solid phase tests for detecting autoantibodies needed to confirm the diagnosisof autoimmune diseases. Utilization of cells or tissue sections as the antigen source makes these assays capable of detecting antibodiesto delicate epitopes masked in other tests such as enzyme linked immunosorbent assay or immunoblot. However, conventional microscopicevaluation of the fluorescent patterns is time consuming and prone to transcription error.In our work we compared four automated fluorescence microscopes (Helios-Aesku Diagnostics, NovaView-Werfen Group, Europattern-Euroimmun, Image Navigator-Immunoconcept) regarding the number of available antigen substrates, specifications of the image capture andanalysis system, extent of the automation, patient safety and impact on the daily routine workflow.All four systems are able to detect antinuclear (ANA) and anti-cytoplasmic antibodies on HEp-2 epithelial cells (positive/negative discriminationonly) while Europattern and NovaView also help with the recognition of the most frequent ANA patterns. The sensitivity ofANA positive/negative discrimination is rather similar between the systems (around 95%), while the specificity varies between 85 to 95%.The capability for detection of anti-neutrophil cytoplasmic antibodies (ANCA) and image capture from other substrates like rat liver-kidneystomach(LKS) shows variability between the different analyzers. Similarly, the throughput, titer-estimation and physical parameters differsignificantly.Automation of the indirect immunofluorescence autoantibody testing is beneficial. In addition to slide processing, several fluorescencemicroscopes are available now which are able to digitize and archive IFA images. Their image analysis software helps to evaluate samples,making the indirect immunofluorescence method less laborious and error-prone.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
Megjelenés:Clinical Chemistry and Laboratory Medicine 54 : 10 (2016), p. 202. -
További szerzők:Csípő István (1953-) (vegyész) Kappelmayer János (1960-) (absztraktok) Antal-Szalmás Péter (1968-) (laboratóriumi szakorvos)
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