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001-es BibID:	BIBFORM019692
Első szerző:	Bácsi Attila (immunológus)
Cím:	Placental macrophage contact potentiates the complete replicative cycle of human cytomegalovirus in syncytiotrophoblast cells : role of interleukin-8 and transforming growth factor-beta 1 / Bácsi A., Aranyosi J., Beck Z., Ebbesen P., Andirkó I., Szabó J., Lampé L., Kiss J., Gergely L., D. Tóth F.
Dátum:	1999
ISSN:	1079-9907
Megjegyzések:	Although syncytiotrophoblast (ST) cells can be infected by human cytomegalovirus (HCMV), in vitro studies have indicated that ST cells do not support the complete viral reproductive cycle, or HCMV replication may occur in less than 3% of ST cells. The present study tested the possibility that placental macrophages might enhance activation of HCMV carried in ST cells and, further, that infected ST cells would be capable of transmitting virus to neighboring macrophages. For this purpose, we studied HCMV replication in ST cells grown alone or cocultured with uninfected placental macrophages. Our results demonstrated that HCMV gene expression in ST cells was markedly upregulated by coculture with macrophages, resulting in release of substantial amounts of infectious virus from HCMV-infected ST cells. After having become permissive for viral replication, ST cells delivered HCMV to the cocultured macrophages, as evidenced by detection of virus-specific antigens in these cells. The stimulatory effect of coculture on HCMV gene expression in ST cells was mediated by marked interleukin-8 (IL-8) and transforming growth factor-beta1 (TGF-beta1) release from macrophages, an effect caused by contact between the different placental cells. Our findings indicate an interactive role for the ST layer and placental macrophages in the dissemination of HCMV among placental tissue. Eventually, these interactions may contribute to the transmission of HCMV from mother to the fetus.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban egyetemen (Magyarországon) készült közlemény
Megjelenés:	Journal Of Interferon And Cytokine Research. - 19 : 10 (1999), p. 1153-1160. -
További szerzők:	Aranyosi János (1963-) (szülész-nőgyógyász) ifj. Beck Zoltán (1970-) (molekuláris biológus, mikrobiológus) Ebbesen, Peter Andirkó István Szabó Judit (1963-) (szakorvos, klinikai mikrobiológus) Lampé László (1929-2021) (szülész-nőgyógyász) Kiss Jolán Gergely Lajos (1940-) (szakorvos, klinikai mikrobiológus) Tóth Ferenc, D. (1940-2004) (mikrobiológus, élettanász)
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001-es BibID:	BIBFORM019693
Első szerző:	Szabó Judit (szakorvos, klinikai mikrobiológus)
Cím:	Role of interleukin-8 and transforming growth factor-beta1 in enhancement of human cytomegalovirus replication by human T cell leukemia-lymphoma virus type I in macrophages coinfected with both viruses / Szabó J., Bácsi A., Beck Z., Kiss J., Andirkó I., D. Tóth F.
Dátum:	1999
Megjegyzések:	Human cytomegalovirus (HCMV) is one of the most frequent opportunistic agents causing severe illness in chronic human T cell leukemia-lymphoma virus type I (HTLV-I) infection. Our previous studies have shown that coinfection of macrophages with HCMV and HTLV-I significantly enhances HCMV replication, resulting in release of infectious HCMV from dually infected cells. We found that double infection of macrophages with HCMV and HTLV-I induced a rapid production of substantial amounts of interleukin-8 (IL-8) and transforming growth factor-beta1 (TGF-beta1). Results of transfection studies demonstrated that the tax gene product of HTLV-I was able to induce secretion of IL-8 and TGF-beta1. In addition to its cytokine-inducing effect, the Tax protein could interact with HCMV synergistically to result in production of much higher levels of IL-8 and TGF-beta1 than expected on the basis of their separate activities. Treatment of dually infected macrophage cultures with neutralizing antibodies to IL-8 and TGF-beta1 led to a nearly 1000-fold decrease in release of infectious HCMV from coinfected cells. Similar results were obtained when anti-IL-8 and anti-TGF-beta1 treatments were combined in macrophage cultures transfected with the tax gene before HCMV infection. Our results suggest that the stimulatory effect of HTLV-I Tax protein on HCMV replication in coinfected macrophages is largely mediated by high levels of IL-8 and TGF-beta1 production.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban egyetemen (Magyarországon) készült közlemény
Megjelenés:	Journal of Interferon & Cytokine Research. - 19 : 2 (1999), p. 209-217. -
További szerzők:	Bácsi Attila (1967-) (immunológus) Beck Zoltán (1970-) (molekuláris biológus, mikrobiológus) Kiss Jolán Andirkó István Tóth Ferenc, D. (1940-2004) (mikrobiológus, élettanász)
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