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001-es BibID:	BIBFORM021666
Első szerző:	Bácsi Attila (immunológus)
Cím:	Pseudotypes of vesicular Stomatitis Virus-bearing envelope antigens of certain HIV-1 strains permissively infect human syncytiotrophoblasts cultured in vitro : implications for in vivo infection of syncytiotrophoblasts by cell-free HIV-1 / Bácsi Attila, Ebbesen Peter, Szabó Judit, Beck Zoltán, Andirkó István, Csoma Eszter, D. Tóth Ferenc
Dátum:	2001
Megjegyzések:	Intrauterine infection of the fetus is clearly an important mode of vertical transmission of human immunodeficiency virus type 1 (HIV-1). The syncytiotrophoblast layer of the human placenta must be traversed by HIV-1 in order to reach underlying cells and fetal capillaries. Although HIV-1 has been detected in the syncytiotrophoblast layer in situ, there is conflicting evidence regarding infection of syncytiotrophoblast cells with cell-free virus. The phenotypic mixing between HIV-1 and vesicular stomatitis virus (VSV) has been exploited to assay the susceptibility of human term syncytiotrophoblast cells to penetration by various strains of HIV-1. VSV(HIV-1(IIIB)) and VSV(HIV-1(Ba-L)) pseudotypes were found to enter syncytiotrophoblast cells. In contrast, VSV pseudotyped with envelope glycoproteins of RF, MN, or Ada-M strains of HIV-1 did not infect syncytiotrophoblasts. Plating efficiency of VSV(HIV-1(IIIB)) and VSV(HIV-1(Ba-L)) was 10-fold lower on syncytiotrophoblasts than on T-cells and macrophages, respectively. Incubation of VSV(HIV-1(IIIB)) and VSV(HIV-1(Ba-L)) viruses with appropriate HIV-1 neutralizing sera before infection strongly inhibited entry of pseudotyped VSV into syncytiotrophoblast cells. These findings demonstrated that infection of syncytiotrophoblasts with VSV(HIV-1) pseudotypes was mediated by Env from IIIB and Ba-L strains of HIV-1. Monoclonal antibodies (MAb) to CD4, CXCR4, CCR5, and CCR3 were tested for their ability to block VSV(HIV-1) infection of syncytiotrophoblast cells. Neither the anti-CD4 nor the anti-CXCR4, anti-CCR5, and anti-CCR3 MAb had any inhibitory effect on infection of syncytiotrophoblast cells with VSV(HIV-1) pseudotypes. Results from this study suggest that cell-free HIV-1 can enter syncytiotrophoblasts and the susceptibility of these cells to penetration by the virus is strain dependent. Pseudotype infection merely demonstrates that the first steps in HIV-1 replication are possible in syncytiotrophoblast cells.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban egyetemen (Magyarországon) készült közlemény
Megjelenés:	Journal of Medical Virology. - 64 : 4 (2001), p. 387-397. -
További szerzők:	Ebbesen, Peter Szabó Judit (1963-) (szakorvos, klinikai mikrobiológus) Beck Zoltán (1970-) (molekuláris biológus, mikrobiológus) Andirkó István Csoma Eszter (1978-) (molekuláris biológus, mikrobiológus) Tóth Ferenc, D. (1940-2004) (mikrobiológus, élettanász)
Pályázati támogatás:	T30185 OTKA
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001-es BibID:	BIBFORM038603
Első szerző:	Csoma Eszter (molekuláris biológus, mikrobiológus)
Cím:	Human herpesvirus 6 variant A infects human term syncytiotrophoblast in vitro and induces replication of human immunodeficiency virus type 1 in dually infected cells / Csoma Eszter, Bácsi Attila, Liu Xiangdong, Szabó Judit, Ebbesen Peter, Beck Zoltán, Kónya József, Andirkó István, Nagy Etelka, D. Tóth Ferenc
Dátum:	2002
ISSN:	0146-6615
Megjegyzések:	Human herpesvirus 6 (HHV-6) and human immunodeficiency virus type 1 (HIV-1) may interact during transplacental transmission of HIV-1. The placental syncytiotrophoblast layer serves as the first line of defense of the fetus against viruses. Patterns of replication of HHV-6 variant A (HHV-6A) and HIV-1 were analyzed in singly and dually infected human term syncytiotrophoblast cells cultured in vitro. For this purpose, the GS strain of HHV-6A and the Ba-L and IIIB strains of HIV-1 were used. HHV-6A replication was restricted at the level of early gene products in singly infected syncytiotrophoblasts, whereas no viral protein expression was found in cells infected with HIV-1 alone. Coinfection of syncytiotrophoblast cells with HHV-6A and HIV-1 resulted in production of infectious HIV-1. In contrast, no enhancement of HHV-6A expression was observed in cell cultures infected with both viruses. Uninfected syncytiotrophoblast cells were found to express CXCR4 and CCR3 but not CD4 or CCR5 receptors. Infection of syncytiotrophoblasts with HHV-6A did not induce CD4 expression and had no influence on chemokine receptor expression. Activation of HIV-1 from latency in coinfected cells was mediated by the immediate-early (IE)-A and IE-B gene products of HHV-6A. Open reading frames U86 and U89 of the IE-A region were able to activate HIV-1 replication in a synergistic manner. The data suggest that in vivo double infection of syncytiotrophoblast cells with HHV-6A and HIV-1 could contribute to the transplacental transmission of HIV-1 but not HHV-6A.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal Of Medical Virology. - 67 : 1 (2002), p. 67-87. -
További szerzők:	Bácsi Attila (1967-) (immunológus) Liu, Xiangdong Szabó Judit (1963-) (szakorvos, klinikai mikrobiológus) Ebbesen, Peter Beck Zoltán (1970-) (molekuláris biológus, mikrobiológus) Kónya József (1964-) (szakorvos, klinikai mikrobiológus) Andirkó István Nagy Etelka Tóth Ferenc, D. (1940-2004) (mikrobiológus, élettanász)
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001-es BibID:	BIBFORM019700
Első szerző:	Tóth Ferenc, D. (mikrobiológus, élettanász)
Cím:	Epstein-Barr virus permissively infects human syncytiotrophoblasts in vitro and induces replication of human T cell leukemia-lymphoma virus type I in dually infected cells / D. Tóth F., Aboagye-Mathiesen G., Nemes J., Liu X., Andirkó I., Hager H., Zdravkovic M., Szabó J., Kiss J., Aranyosi J., Ebbesen P.
Dátum:	1997
ISSN:	0042-6822
Megjegyzések:	Epstein-Barr virus (EBV) and human immunodeficiency virus type 1 (HIV-1), as well as human T-cell leukemia-lymphoma virus type I (HTLV-I), may interact in the pathogenesis of human retroviral infections. The placental syncytiotrophoblast layer represents a barrier protecting the fetal compartment from exposure to retroviruses. We studied the interactions of EBV with HIV-1 and HTLV-I in human term syncytiotrophoblast cells to investigate the significance of double infections in transplacental transmission of human retroviruses. We found that syncytiotrophoblast cells could be productively infected with EBV. Dual infection of the cells with EBV and HTLV-I resulted in full replication cycle of otherwise latent HTLV-I. In contrast, the restricted permissiveness of syncytiotrophoblasts for HIV-1 was not influenced by coinfection of the cells with EBV. Infection of syncytiotrophoblast cells with EBV, but not HTLV-I, induced interleukin-2 and interleukin-6 secretion, and augmented secretion occurred on coinfection with both viruses. Coinfection of syncytiotrophoblast cells with EBV and HTLV-I induced tumor necrosis factor-beta and transforming growth factor-beta 1 secretion, but infection with either virus alone did not lead to secretion of these cytokines. Permissive replication cycle of HTLV-I was induced by the EBV immediate-early gene product Zta. Pseudotype formation between EBV and HTLV-I in coinfected syncytiotrophoblast cells was not found. Our data suggest that activation of HTLV-I gene expression by EBV in coinfected syncytiotrophoblast cells may be a mechanism for transplacental transmission of HTLV-I.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban külföldön készült közlemény
Megjelenés:	Virology. - 229 : 2 (1997), p. 400-414. -
További szerzők:	Aboagye-Mathiesen, George Nemes József Liu, Xiangdong Andirkó István Hager Henrik Zdravkovic, Milan Szabó Judit (1963-) (szakorvos, klinikai mikrobiológus) Kiss Jolán Aranyosi János (1963-) (szülész-nőgyógyász) ifj. Ebbesen, Peter
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