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1.

001-es BibID:BIBFORM021666
Első szerző:Bácsi Attila (immunológus)
Cím:Pseudotypes of vesicular Stomatitis Virus-bearing envelope antigens of certain HIV-1 strains permissively infect human syncytiotrophoblasts cultured in vitro : implications for in vivo infection of syncytiotrophoblasts by cell-free HIV-1 / Bácsi Attila, Ebbesen Peter, Szabó Judit, Beck Zoltán, Andirkó István, Csoma Eszter, D. Tóth Ferenc
Dátum:2001
Megjegyzések:Intrauterine infection of the fetus is clearly an important mode of vertical transmission of human immunodeficiency virus type 1 (HIV-1). The syncytiotrophoblast layer of the human placenta must be traversed by HIV-1 in order to reach underlying cells and fetal capillaries. Although HIV-1 has been detected in the syncytiotrophoblast layer in situ, there is conflicting evidence regarding infection of syncytiotrophoblast cells with cell-free virus. The phenotypic mixing between HIV-1 and vesicular stomatitis virus (VSV) has been exploited to assay the susceptibility of human term syncytiotrophoblast cells to penetration by various strains of HIV-1. VSV(HIV-1(IIIB)) and VSV(HIV-1(Ba-L)) pseudotypes were found to enter syncytiotrophoblast cells. In contrast, VSV pseudotyped with envelope glycoproteins of RF, MN, or Ada-M strains of HIV-1 did not infect syncytiotrophoblasts. Plating efficiency of VSV(HIV-1(IIIB)) and VSV(HIV-1(Ba-L)) was 10-fold lower on syncytiotrophoblasts than on T-cells and macrophages, respectively. Incubation of VSV(HIV-1(IIIB)) and VSV(HIV-1(Ba-L)) viruses with appropriate HIV-1 neutralizing sera before infection strongly inhibited entry of pseudotyped VSV into syncytiotrophoblast cells. These findings demonstrated that infection of syncytiotrophoblasts with VSV(HIV-1) pseudotypes was mediated by Env from IIIB and Ba-L strains of HIV-1. Monoclonal antibodies (MAb) to CD4, CXCR4, CCR5, and CCR3 were tested for their ability to block VSV(HIV-1) infection of syncytiotrophoblast cells. Neither the anti-CD4 nor the anti-CXCR4, anti-CCR5, and anti-CCR3 MAb had any inhibitory effect on infection of syncytiotrophoblast cells with VSV(HIV-1) pseudotypes. Results from this study suggest that cell-free HIV-1 can enter syncytiotrophoblasts and the susceptibility of these cells to penetration by the virus is strain dependent. Pseudotype infection merely demonstrates that the first steps in HIV-1 replication are possible in syncytiotrophoblast cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of Medical Virology. - 64 : 4 (2001), p. 387-397. -
További szerzők:Ebbesen, Peter Szabó Judit (1963-) (szakorvos, klinikai mikrobiológus) Beck Zoltán (1970-) (molekuláris biológus, mikrobiológus) Andirkó István Csoma Eszter (1978-) (molekuláris biológus, mikrobiológus) Tóth Ferenc, D. (1940-2004) (mikrobiológus, élettanász)
Pályázati támogatás:T30185
OTKA
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
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2.

001-es BibID:BIBFORM019692
Első szerző:Bácsi Attila (immunológus)
Cím:Placental macrophage contact potentiates the complete replicative cycle of human cytomegalovirus in syncytiotrophoblast cells : role of interleukin-8 and transforming growth factor-beta 1 / Bácsi A., Aranyosi J., Beck Z., Ebbesen P., Andirkó I., Szabó J., Lampé L., Kiss J., Gergely L., D. Tóth F.
Dátum:1999
ISSN:1079-9907
Megjegyzések:Although syncytiotrophoblast (ST) cells can be infected by human cytomegalovirus (HCMV), in vitro studies have indicated that ST cells do not support the complete viral reproductive cycle, or HCMV replication may occur in less than 3% of ST cells. The present study tested the possibility that placental macrophages might enhance activation of HCMV carried in ST cells and, further, that infected ST cells would be capable of transmitting virus to neighboring macrophages. For this purpose, we studied HCMV replication in ST cells grown alone or cocultured with uninfected placental macrophages. Our results demonstrated that HCMV gene expression in ST cells was markedly upregulated by coculture with macrophages, resulting in release of substantial amounts of infectious virus from HCMV-infected ST cells. After having become permissive for viral replication, ST cells delivered HCMV to the cocultured macrophages, as evidenced by detection of virus-specific antigens in these cells. The stimulatory effect of coculture on HCMV gene expression in ST cells was mediated by marked interleukin-8 (IL-8) and transforming growth factor-beta1 (TGF-beta1) release from macrophages, an effect caused by contact between the different placental cells. Our findings indicate an interactive role for the ST layer and placental macrophages in the dissemination of HCMV among placental tissue. Eventually, these interactions may contribute to the transmission of HCMV from mother to the fetus.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal Of Interferon And Cytokine Research. - 19 : 10 (1999), p. 1153-1160. -
További szerzők:Aranyosi János (1963-) (szülész-nőgyógyász) ifj. Beck Zoltán (1970-) (molekuláris biológus, mikrobiológus) Ebbesen, Peter Andirkó István Szabó Judit (1963-) (szakorvos, klinikai mikrobiológus) Lampé László (1929-2021) (szülész-nőgyógyász) Kiss Jolán Gergely Lajos (1940-) (szakorvos, klinikai mikrobiológus) Tóth Ferenc, D. (1940-2004) (mikrobiológus, élettanász)
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3.

001-es BibID:BIBFORM055859
Első szerző:Beck Zoltán (molekuláris biológus, mikrobiológus)
Cím:Interactions between HTLV type I and HIV type 1 in monocyte-derived macrophages cultured in vitro / Beck Zoltán, Szabó Judit, Liu Xiangdong, Bácsi Attila, Ebbesen Peter, D. Tóth Ferenc
Dátum:2001
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:AIDS Research And Human Retroviruses. - 17 : Suppl. 1 (2001), p. 51. -
További szerzők:Szabó Judit (1963-) (szakorvos, klinikai mikrobiológus) Liu, Xiangdong Bácsi Attila (1967-) (immunológus) Ebbesen, Peter Tóth Ferenc, D. (1940-2004) (mikrobiológus, élettanász)
Borító:

4.

001-es BibID:BIBFORM038603
Első szerző:Csoma Eszter (molekuláris biológus, mikrobiológus)
Cím:Human herpesvirus 6 variant A infects human term syncytiotrophoblast in vitro and induces replication of human immunodeficiency virus type 1 in dually infected cells / Csoma Eszter, Bácsi Attila, Liu Xiangdong, Szabó Judit, Ebbesen Peter, Beck Zoltán, Kónya József, Andirkó István, Nagy Etelka, D. Tóth Ferenc
Dátum:2002
ISSN:0146-6615
Megjegyzések:Human herpesvirus 6 (HHV-6) and human immunodeficiency virus type 1 (HIV-1) may interact during transplacental transmission of HIV-1. The placental syncytiotrophoblast layer serves as the first line of defense of the fetus against viruses. Patterns of replication of HHV-6 variant A (HHV-6A) and HIV-1 were analyzed in singly and dually infected human term syncytiotrophoblast cells cultured in vitro. For this purpose, the GS strain of HHV-6A and the Ba-L and IIIB strains of HIV-1 were used. HHV-6A replication was restricted at the level of early gene products in singly infected syncytiotrophoblasts, whereas no viral protein expression was found in cells infected with HIV-1 alone. Coinfection of syncytiotrophoblast cells with HHV-6A and HIV-1 resulted in production of infectious HIV-1. In contrast, no enhancement of HHV-6A expression was observed in cell cultures infected with both viruses. Uninfected syncytiotrophoblast cells were found to express CXCR4 and CCR3 but not CD4 or CCR5 receptors. Infection of syncytiotrophoblasts with HHV-6A did not induce CD4 expression and had no influence on chemokine receptor expression. Activation of HIV-1 from latency in coinfected cells was mediated by the immediate-early (IE)-A and IE-B gene products of HHV-6A. Open reading frames U86 and U89 of the IE-A region were able to activate HIV-1 replication in a synergistic manner. The data suggest that in vivo double infection of syncytiotrophoblast cells with HHV-6A and HIV-1 could contribute to the transplacental transmission of HIV-1 but not HHV-6A.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal Of Medical Virology. - 67 : 1 (2002), p. 67-87. -
További szerzők:Bácsi Attila (1967-) (immunológus) Liu, Xiangdong Szabó Judit (1963-) (szakorvos, klinikai mikrobiológus) Ebbesen, Peter Beck Zoltán (1970-) (molekuláris biológus, mikrobiológus) Kónya József (1964-) (szakorvos, klinikai mikrobiológus) Andirkó István Nagy Etelka Tóth Ferenc, D. (1940-2004) (mikrobiológus, élettanász)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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5.

001-es BibID:BIBFORM055856
Első szerző:Szabó Judit (szakorvos, klinikai mikrobiológus)
Cím:Interaction between human immuno-deficiency virus type 1 (HIV-1) and human T-cell leukaemia-lymphoma virus type I (HTLV-I) in macrophages cultured in vitro : implications for in vivo pathogenesis / Szabó Judit, Beck Zoltán, Csomán Éva, Liu Xiangdong, Andirkó István, Kiss Jolán, Bácsi Attila, Ebbesen Peter, D. Tóth Ferenc
Dátum:2000
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Acta Microbiologica et Immunologica Hungarica. - 47 (2000), p. 240. -
További szerzők:Beck Zoltán (1970-) (molekuláris biológus, mikrobiológus) Csomán Éva Liu, Xiangdong Andirkó István Kiss Jolán Bácsi Attila (1967-) (immunológus) Ebbesen, Peter Tóth Ferenc, D. (1940-2004) (mikrobiológus, élettanász)
Borító:

6.

001-es BibID:BIBFORM019688
Első szerző:Szabó Judit (szakorvos, klinikai mikrobiológus)
Cím:Differential patterns of interaction between HIV type 1 and HTLV type I in monocyte-derived macrophages cultured in vitro : implications for in vivo coinfection with HIV type 1 and HTLV type I / Szabó J., Beck Z., Csomán É., Liu X., Andirkó I., Kiss J., Bácsi A., Ebbesen P., D. Tóth F.
Dátum:1999
ISSN:0889-2229
Megjegyzések:The interaction between human immunodeficiency virus type 1 (HIV-1) and human T cell leukemia-lymphoma virus type I (HTLV-I) has generated substantial interest. However, there is disagreement on the in vivo consequences of the double infection. We investigated the interactions between HIV-1 and HTLV-I in monocyte-derived macrophages cultured in vitro . For study, the T cell-tropic strain IIIB and the macrophagetropic strain Ada-M of HIV-1 were used. The HTLV-I was prepared from the supernatants of the virus-producing MT-2 cell line. We found that coinfection of macrophages with T cell-tropic HIV-1 and HTLV-I significantly enhanced HIV-1 replication, whereas double infection of the cells with macrophage-tropic HIV-1 and HTLV-I resulted in marked upregulation of HTLV-I production. Stimulatory interactions between HIV1 and HTLV-I were mediated by their trans -acting proteins. Results of study on nuclear translocation of proviral DNA showed that the tax gene product of HTLV-I was able to facilitate the nuclear import of the reversetranscribed HIV-1IIIB DNA. In contrast, the HIV-1 Tat protein did not increase the intranuclear trafficking of HTLV-I DNA, which suggests another mechanism for HTLV-I enhancement by the tat gene product. In conclusion, this study provides possible mechanisms whereby coinfection of an individual with HIV-1 and HTLV-I may influence the clinical outcome of double infection.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Aids Research And Human Retroviruses. - 15 : 18 (1999), p. 1653-1666. -
További szerzők:Beck Zoltán (1970-) (molekuláris biológus, mikrobiológus) Csomán Éva Liu, Xiangdong Andirkó István Kiss Jolán Bácsi Attila (1967-) (immunológus) Ebbesen, Peter Tóth Ferenc, D. (1940-2004) (mikrobiológus, élettanász)
Borító:

7.

001-es BibID:BIBFORM019699
Első szerző:Tóth Ferenc, D. (mikrobiológus, élettanász)
Cím:Coinfection as a factor in vertical viral transmission / D. Tóth F., Aboagye-Mathiesen G., Szabó J., Liu X., Mosborg-Petersen P., Kiss J., Zdravkovic M., Hager H., Ebbesen P.
Dátum:1998
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:Placenta (Annual Supplement: Trophoblast Research). - 19 : Suppl.2. (1998), p. 171-190. -
További szerzők:Aboagye-Mathiesen, George Szabó Judit (1963-) (szakorvos, klinikai mikrobiológus) Liu, Xiangdong Mosborg-Petersen, P. Kiss Jolán Zdravkovic, Milan Hager Henrik Ebbesen, Peter
Internet cím:DOI
Borító:

8.

001-es BibID:BIBFORM019702
Első szerző:Tóth Ferenc, D. (mikrobiológus, élettanász)
Cím:Bidirectional enhancing activities between human cell leukemia-lymphoma virus type I and human cytomegalovirus in human term syncytiotrophoblast cells cultured in vitro / D. Tóth F., Aboagye-Mathiesen G., Szabó J., Liu X., Mosborg-Petersen P., Kiss J., Hager H., Zdravkovic M., Andirkó I., Aranyosi J., Ebbesen P.
Dátum:1995
ISSN:0889-2229
Megjegyzések:The syncytiotrophoblast layer of the human placenta has an important role in limiting transplacental viral spread from mother to fetus. Human cytomegalovirus (HCMV) is capable of establishing a latent infection in syncytiotrophoblast cells, with restriction of gene expression to immediate-early and early proteins. We analyzed the extent of replication of human T cell leukemia-lymphoma virus type I (HTLV-I) in human term syncytiotrophoblasts infected with HTLV-I alone or coinfected with HTLV-I and HCMV. Although syncytiotrophoblasts could be infected with cell-free HTLV-I, no viral protein expression was found in the singly infected cells. On the contrary, coinfection of the cells with HTLV-I and HCMV resulted in simultaneous replication of both viruses. Bidirectional enhancing activities between HTLV-I and HCMV were mediated primarily by the Tax and immediate-early proteins, respectively. The stimulatory effect of HTLV-I Tax on HCMV replication appeared to be mediated partly by tumor necrosis factor beta and transforming growth factor beta-1. We observed formation of pseudotypes with HTLV-I nucleocapsids within HCMV envelopes, whereas HCMV was not pseudotyped by HTLV-I envelopes in dually infected syncytiotrophoblast cells. Our data suggest that in vivo dual infection of syncytiotrophoblast cells with HTLV-I and HCMV may facilitate the transplacental transmission of both viruses.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:Aids Research And Human Retroviruses. - 11 : 12 (1995), p. 1495-1507. -
További szerzők:Aboagye-Mathiesen, George Szabó Judit (1963-) (szakorvos, klinikai mikrobiológus) Liu, Xiangdong Mosborg-Petersen, P. Kiss Jolán Hager Henrik Zdravkovic, Milan Andirkó István Aranyosi János (1963-) (szülész-nőgyógyász) ifj. Ebbesen, Peter
Borító:

9.

001-es BibID:BIBFORM019700
Első szerző:Tóth Ferenc, D. (mikrobiológus, élettanász)
Cím:Epstein-Barr virus permissively infects human syncytiotrophoblasts in vitro and induces replication of human T cell leukemia-lymphoma virus type I in dually infected cells / D. Tóth F., Aboagye-Mathiesen G., Nemes J., Liu X., Andirkó I., Hager H., Zdravkovic M., Szabó J., Kiss J., Aranyosi J., Ebbesen P.
Dátum:1997
ISSN:0042-6822
Megjegyzések:Epstein-Barr virus (EBV) and human immunodeficiency virus type 1 (HIV-1), as well as human T-cell leukemia-lymphoma virus type I (HTLV-I), may interact in the pathogenesis of human retroviral infections. The placental syncytiotrophoblast layer represents a barrier protecting the fetal compartment from exposure to retroviruses. We studied the interactions of EBV with HIV-1 and HTLV-I in human term syncytiotrophoblast cells to investigate the significance of double infections in transplacental transmission of human retroviruses. We found that syncytiotrophoblast cells could be productively infected with EBV. Dual infection of the cells with EBV and HTLV-I resulted in full replication cycle of otherwise latent HTLV-I. In contrast, the restricted permissiveness of syncytiotrophoblasts for HIV-1 was not influenced by coinfection of the cells with EBV. Infection of syncytiotrophoblast cells with EBV, but not HTLV-I, induced interleukin-2 and interleukin-6 secretion, and augmented secretion occurred on coinfection with both viruses. Coinfection of syncytiotrophoblast cells with EBV and HTLV-I induced tumor necrosis factor-beta and transforming growth factor-beta 1 secretion, but infection with either virus alone did not lead to secretion of these cytokines. Permissive replication cycle of HTLV-I was induced by the EBV immediate-early gene product Zta. Pseudotype formation between EBV and HTLV-I in coinfected syncytiotrophoblast cells was not found. Our data suggest that activation of HTLV-I gene expression by EBV in coinfected syncytiotrophoblast cells may be a mechanism for transplacental transmission of HTLV-I.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:Virology. - 229 : 2 (1997), p. 400-414. -
További szerzők:Aboagye-Mathiesen, George Nemes József Liu, Xiangdong Andirkó István Hager Henrik Zdravkovic, Milan Szabó Judit (1963-) (szakorvos, klinikai mikrobiológus) Kiss Jolán Aranyosi János (1963-) (szülész-nőgyógyász) ifj. Ebbesen, Peter
Internet cím:DOI
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