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1.

001-es BibID:BIBFORM007800
Első szerző:Benkő Zsigmond (molekuláris biológus, genetikus)
Cím:Caffeine-resistance in S. pombe: mutations in three novel caf genes increase caffeine tolerance and affect radiation sensitivity, fertility, and cell cycle / Z. Benko, I. Miklos I., Antony M. Carr, Matthias Sipiczki
Dátum:1997
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Current Genetics. - 31 : 6 (1997), p. 481-487. -
További szerzők:Miklós Ida (1962-) (biológus) Carr, Antony M. Sipiczki Mátyás (1948-) (biológus)
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2.

001-es BibID:BIBFORM010284
Első szerző:Miklós Ida (biológus)
Cím:The pleiotropic cell separation mutation spl1-1 is a nucleotide substitution in the internal promoter of the proline tRNA(CGG) gene of Schizosaccharomyces pombe / Miklós Ida, Ludányi Katalin, Matthias Sipiczki
Dátum:2009
Megjegyzések:spl1-1 was originally identified as a spontaneous mutation genetically interacting with sep1-1 and cdc4-8 in producing multinucleate syncytia. This study shows that it is allelic with the proline-tRNA(CGG) gene SPATRNAPRO.02. Its nucleotide sequence contains a C -> T substitution in the region corresponding to the B-box of the putative intragenic promoter and the T psi C loop of the mature tRNA. The substitution drastically reduces the transcription efficiency of the gene and pleiotropically affects numerous cellular processes. spl1-1 cells are temperature sensitive, osmosensitive, bend at higher temperatures, have extended G2 phase and are defective in cell separation (septum cleavage). The proline-tRNA(TGG) gene SPATRNAPRO.01 can partially suppress the spl1-1 mutation when introduced into the cells on a multicopy plasmid. The effect of a mutation in a tRNA gene on cell separation brings a new element into the complexity of the regulation of cell division and its co-ordination with other cellular processes in Schizosaccharomyces pombe.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Cytokinesis
Septum
Transcription
Cell cycle
Fission yeast
tRNA
Megjelenés:Current Genetics. - 55 : 5 (2009), p. 511-520. -
További szerzők:Ludányi Katalin (1975-) (immunológus) Sipiczki Mátyás (1948-) (biológus)
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3.

001-es BibID:BIBFORM096053
Első szerző:Papp László Attila (genetikus)
Cím:Molecular and comparative genomic analyses reveal evolutionarily conserved and unique features of the Schizosaccharomyces japonicus mycelial growth and the underlying genomic changes / Papp László Attila, Ács-Szabó Lajos, Batta Gyula, Miklós Ida
Dátum:2021
ISSN:0172-8083
Megjegyzések:Fungal pathogens, from phytopathogenic fungus to human pathogens, are able to alternate between the yeast-like form and filamentous forms. This morphological transition (dimorphism) is in close connection with their pathogenic lifestyles and with their responses to changing environmental conditions. The mechanisms governing these morphogenetic conversions are still not fully understood. Therefore, we studied the filamentous growth of the less-known, non-pathogenic dimorphic fission yeast, S. japonicus, which belongs to an ancient and early evolved branch of the Ascomycota. Its RNA sequencing revealed that several hundred genes were up- or down-regulated in the hyphae compared to the yeast-phase cells. These genes belonged to different GO categories, confirming that mycelial growth is a rather complex process. The genes of transport- and metabolic processes appeared especially in high numbers among them. High expression of genes involved in glycolysis and ethanol production was found in the hyphae, while other results pointed to the regulatory role of the protein kinase A (PKA) pathway. The homologues of 49 S. japonicus filament-associated genes were found by sequence alignments also in seven distantly related dimorphic and filamentous species. The comparative genomic analyses between S. japonicus and the closely related but non-dimorphic S. pombe shed some light on the differences in their genomes. All these data can contribute to a better understanding of hyphal growth and those genomic rearrangements that underlie it.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Bioinformatic analysis
Dimorphism
Hyphae
Mycelial growth
Schizosaccharomyces japonicus
Transcriptional profiling
Megjelenés:Current Genetics. - 67 : 6 (2021), p. 953-968. -
További szerzők:Ács-Szabó Lajos (1985-) (biológus) Batta Gyula (1979-) (biológus) Miklós Ida (1962-) (biológus)
Pályázati támogatás:EFOP-3.6.1-16-2016-00022
EFOP
20428-3/2018
FIKP
K10172
OTKA
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DOI
Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:BIBFORM093840
Első szerző:Papp László Attila (genetikus)
Cím:A modified culture medium and hyphae isolation method can increase quality of the RNA extracted from mycelia of a dimorphic fungal species / Papp László Attila, Ács-Szabó Lajos, Póliska Szilárd, Miklós Ida
Dátum:2021
ISSN:0172-8083
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Current Genetics. - 67 : 5 (2021), p. 823-830. -
További szerzők:Ács-Szabó Lajos (1985-) (biológus) Póliska Szilárd (1978-) (biológus) Miklós Ida (1962-) (biológus)
Pályázati támogatás:OTKA-106172
OTKA
EFOP-3.6.1-16-2016-00022
EFOP
20428-3/2018
FIKP
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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5.

001-es BibID:BIBFORM081302
Első szerző:Papp László Attila (genetikus)
Cím:Expression pattern and phenotypic characterization of the mutant strain reveals target genes and processes regulated by pka1 in the dimorphic fission yeast Schizosaccharomyces japonicus / László Papp, Matthias Sipiczki, Ida Miklós
Dátum:2017
ISSN:0172-8083
Megjegyzések:The cAMP cascade plays an important role in several biological processes. Thus, study of its molecular details can contribute to a better understanding of these processes, treatment of diseases, or even finding antifungal drug targets. To gain further information about the PKA pathway, and its evolutionarily conserved and species-specific features, the central regulator pka1 gene, which encodes the cAMP-dependent protein kinase catalytic subunit, was studied in the less known haplontic, dimorphic fission yeast Schizosaccharomyces japonicus. Namely, this species belongs to a highly divergent phylogenetic branch of fungi. Furthermore, S. japonicus had only a single copy pka1 gene in contrast to the budding yeasts. Therefore, the pka1 deleted mutant was created, whose RNA sequencing and phenotypic studies revealed that the Pka1 regulated at least 373 genes, among them further kinases, phosphatases and transcriptional regulators. It regulated elongation of hyphae, cell size, aging and stress response. Furthermore, half of the pka1 target genes seemed to be conserved in Schizosaccharomyces pombe and S. japonicus. However, there were oppositely regulated genes in the two closely related species. The target genes suggest that this single gene must be able to fulfill all the functions of TPK1-3 of Saccharomyces cerevisiae. Thus, our results shed light on certain similarities and differences of the PKA pathway of S. japonicus compared to the budding yeasts and confirmed the multifunctionality of the pka1 gene, but further experiments are needed to prove its involvement in the metabolic processes and transport.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
pka1
cAMP cascade
Protein kinase
RNA sequencing
Dimorphism
Schizosaccharomyces japonicus
Megjelenés:Current Genetics. - 63 : 3 (2017), p. 487-497. -
További szerzők:Sipiczki Mátyás (1948-) (biológus) Miklós Ida (1962-) (biológus)
Pályázati támogatás:OTKA K106172
OTKA
SROP-4.2.2.B-15/1/KONV-2015-0001
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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6.

001-es BibID:BIBFORM070493
Első szerző:Pataki Emese (molekuláris biológus)
Cím:fhl1 gene of the fission yeast regulates transcription of meiotic genes and nitrogen starvation response, downstream of the TORC1 pathway / Emese Pataki, Ronit Weisman, Matthias Sipiczki, Ida Miklos
Dátum:2016
ISSN:0172-8083 1432-0983
Megjegyzések:Environmental changes, such as nutrient limitation or starvation induce different signal transducing pathways, which require coordinated cooperation of several genes. Our previous data revealed that the fhl1 fork-head type transcription factor of the fission yeast could be involved in sporulation, which was typically induced under poor conditions. Since the exact role of Fhl1 in this process was not known, we wanted to identify its downstream targets and to investigate its possible cooperation with another known regulator of sporulation. Gene expression and Northern blot analysis of the fhl1? mutant strain revealed the target genes involved in mating and sporulation. Our results also showed that Fhl1 could regulate nutrient sensing, the transporter and permease genes. Since the majority of these genes belonged to the nitrogen starvation response, the possible cooperation of fhl1 and tor2 was also investigated. Comparison of their microarray data and the expression of fhl1 + from a strong promoter in the tor2-ts mutant cells suggested that one part of the target genes are commonly regulated by Fhl1 and Tor2. Since the expression of fhl1 + from a strong promoter could rescue rapamycin and temperature sensitivity and suppressed the hyper-sporulation defect of the tor2-ts mutant cells, we believe that Fhl1 acts in TOR signaling, downstream of Tor2. Thus, this work shed light on certain novel details of the regulation of the sexual processes and a new member of the TOR pathway, but further experiments are needed to confirm the involvement of Fhl1 in nutrient sensing.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Schizosaccharomyces pombe
TOR pathway
Fork-head transcription factor
nitrogen response
Megjelenés:Current Genetics 63 : 1 (2016), p. 91-101. -
További szerzők:Weisman, Ronit Sipiczki Mátyás (1948-) (biológus) Miklós Ida (1962-) (biológus)
Pályázati támogatás:OTKA-106172
OTKA
TÁMOP 4.2.2.B-15/1/KONV-2015-0001.
TÁMOP
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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7.

001-es BibID:BIBFORM039230
Első szerző:Zilahi Erika (molekuláris biológus)
Cím:The Schizosaccharomyces pombe sep15+ gene encodes a protein homologous to the Med8 subunit of the Saccharomyces cerevisiae transcriptional mediator complex / E. Zilahi, I. Miklos, M. Sipiczki
Dátum:2000
ISSN:0172-8083
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Current Genetics. - 38 (2000), p. 227-232. -
További szerzők:Miklós Ida (1962-) (biológus) Sipiczki Mátyás (1948-) (biológus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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