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001-es BibID:	BIBFORM105800
035-os BibID:	(WoS)000175122400002 (Scopus)0036224981
Első szerző:	Bozsik Anikó
Cím:	Marker construction and cloning of acut1-like sequence with ARS activity in the fission yeastSchizosaccharomyces japonicus / Aniko Bozsik, Z. Szilagyi, Z. Benko, M. Sipiczki
Dátum:	2002
ISSN:	0749-503X
Megjegyzések:	The dimorphic fission yeast Schizosaccharomyces japonicus has proved to be an excellent experimental model for the investigation of the eukaryotic cell. Here we show that it has a haplontic life cycle, in which the diploid phase is confined to the zygote. To make it amenable to genetic and molecular analysis, we generated genetic markers and cloned a genomic sequence which acts as ars when integrated into a plasmid. Diploids suitable for testing complementation and recombination between markers can be formed by protoplast fusion. The complementation tests and the recombination frequencies determined in octads of spores identified 28 non-allelic groups (genes) of mutations of the auxotrophic and mycelium-negative mutants. Two groups of linked markers were also identified. The cloned fragment, which expresses ars activity, encodes a putative amino acid sequence highly similar to a conserved domain of proteins Cut1 (Schizosaccharomyces pombe), BimB (Aspergillus nidulans) and Esp1 (Saccharomyces cerevisiae).
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk fission yeast dimorphism auxotrophy protoplast octad transformation ars: Cut1
Megjelenés:	Yeast. - 19 : 6 (2002), p. 485-498. -
További szerzők:	Szilágyi Zsolt (1975-) (biológus) Benkő Zsigmond (1961-) (molekuláris biológus, genetikus) Sipiczki Mátyás (1948-) (biológus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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