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1.
001-es BibID:
BIBFORM004881
Első szerző:
Olamendi-Portugal, Timoteo
Cím:
Novel alpha-KTx peptides from the venom of the scorpion Centruroides elegans selectively blockade Kv1.3 over IKCa1 K+ channels of T cells / Olamendi-Portugal, T., Somodi, S., Fernandez, J. A., Zamudio, F. Z., Becerril, B., Varga, Z., Panyi, G., Gaspar, R., Possani, L. D.
Dátum:
2005
ISSN:
0041-0101
Megjegyzések:
From the venom of the Mexican scorpion Centruroides elegans Thorell five peptides were isolated to homogeneity by chromatographic procedures and their full amino acid sequence was determined by automatic Edman degradation. They all belong to the Noxiustoxin subfamily of scorpion toxins and were given the systematic names alpha-KTx 2.8 to 2.12, with trivial names Ce1 to Ce5, respectively. They have 39 amino acid residues, except for Ce3 which has only 38, but all of them have three disulfide bridges, and have molecular weights of 4255, 4267, 4249, 4295 and 4255 atomic mass units, respectively for Ce1 to Ce5. The C-terminal residues of Ce2, Ce4 and Ce5 were found to be amidated. The electrophysiological assay (whole-cell patch-clamp) showed that out of the five peptides, Ce1 (alpha-KTx 2.8), Ce2 (alpha-KTX2.9) and Ce4 (alpha-KTx 2.11) were effective blockers of Kv1.3 channels of human T lymphocytes, whereas these peptides did not inhibit the Ca2+-activated K+ channels (IKCa1) of the same cells. The equilibrium dissociation constants of these peptides for Kv1.3 were 0.70, 0.25 and 0.98nM for Ce1, Ce2 and Ce4, respectively. Furthermore, toxins Ce1, Ce2 and Ce4 practically did not inhibit the related voltage gated Shaker K+ channels, and rKv2.1 channels of the Shab family. The high affinity blockage of Kv1.3 channels by these peptides and their selectivity for Kv1.3 over IKCa1 may have significance in the development of novel tools for suppressing the function of those T cell subsets whose proliferation critically depends on the activity of Kv1.3 channels.
Tárgyszavak:
Orvostudományok
Elméleti orvostudományok
idegen nyelvű folyóiratközlemény külföldi lapban
Amino Acid Sequence
Animals
antagonists & inhibitors
Bayes Theorem
Cell Line
Cells
chemistry
Chromatography,High Pressure Liquid
Comparative Study
Electrophysiology
Enzyme-Linked Immunosorbent Assay
genetics
Human
Humans
Intermediate-Conductance Calcium-Activated Potassium Channels
Kv1.3 Potassium Channel
Lymphocytes
Mass Spectrometry
metabolism
Mexico
Models,Genetic
Molecular Sequence Data
Molecular Weight
Organophosphorus Compounds
Peptides
Phylogeny
Potassium
Potassium Channels
Research
Scorpion Venoms
Scorpions
Sequence Analysis,Protein
Support
T-Lymphocytes
toxicity
Toxins
Megjelenés:
Toxicon. - 46 : 4 (2005), p. 418-429. -
További szerzők:
Somodi Sándor (1977-) (belgyógyász)
Fernandez, Juan Antonio
Zamudio, Fernando Z.
Becerril, Baltazar
Varga Zoltán (1969-) (biofizikus, szakfordító)
Panyi György (1966-) (biofizikus)
Gáspár Rezső (1944-) (biofizikus)
Possani, Lourival Domingos
Internet cím:
elektronikus változat
DOI
Borító:
Saját polcon:
2.
001-es BibID:
BIBFORM010358
Első szerző:
Papp Ferenc (biofizikus)
Cím:
Tst26, a novel peptide blocker of Kv1.2 and Kv1.3 channels from the venom of Tityus stigmurus / Papp, F., Batista, C. V. F., Varga, Z., Herceg, M., Roman-Gonzalez, S. A., Gaspar, R., Possani, L. D., Panyi, G.
Dátum:
2009
ISSN:
0041-0101 (Print)
Megjegyzések:
Using high-performance liquid chromatography Tst26, a novel potassium channel blocker peptide, was purified from the venom of the Brazilian scorpion Tityus stigmurus. its primary structure was determined by means of automatic Edman degradation and mass spectrometry analysis. The peptide is composed of 37 amino acid residues and tightly folded through three disulfide bridges, similar to other K+ channel blocking peptides purified from scorpion venoms. It contains the "essential dyad" for K+ channel recognition comprised of a lysine at position 27 and a tyrosine at position 36. Electrophysiological assays revealed that Tst26 blocked hKv1.2 and hKv1.3 channels with high affinity (K-d = 1.9 nM and 10.7 nM, respectively) while it did not affect several other ion channels (mKv1.1, hKv1.4, hKv1.5, hERG, hIKCa1, hBK, hNav1.5) tested at 10 nM concentration. The voltage-dependent steady-state parameters of K+ channel gating were unaffected by the toxin in both channels, but due to the fast association and dissociation kinetics Tst26 slowed the rate of inactivation of Kv1.3 channels. Based on the primary structure, the systematic nomenclature proposed for this peptide is alpha-KTx 4.6.
Tárgyszavak:
Orvostudományok
Elméleti orvostudományok
idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Chromatography
Ion Channels
Kinetics
Lysine
Mass Spectrometry
Peptides
Potassium
Scorpion Venoms
Spectrometry
Tyrosine
Megjelenés:
Toxicon. - 54 : 4 (2009), p. 379-389. -
További szerzők:
Batista, Cesar V. F.
Varga Zoltán (1969-) (biofizikus, szakfordító)
Herceg Mónika (biofizikus)
Roman-Gonzalez, Sergio A.
Gáspár Rezső (1944-) (biofizikus)
Possani, Lourival Domingos
Panyi György (1966-) (biofizikus)
Internet cím:
DOI
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