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1.
001-es BibID:
BIBFORM006034
Első szerző:
Gáspár Rezső (biofizikus)
Cím:
Bretylium-induced voltage-gated sodium current in human lymphocytes / Rezső Gáspár, Zoltán Krasznai, Teréz Márián, Lajos Trón, Rina Recchioni, Marco Falasca, Fausto Moroni, Carlo Pieri, Sándor Damjanovich
Dátum:
1992
ISSN:
0167-4889
Megjegyzések:
Using the whole-cell variation of the patch-clamp technique it has been determined that 0.25-3 mM bretylium tosylate (BT) exerts a repolarizing effect on partially depolarized human lymphocytes. The repolarizing effect was ouabain (40 microM)-sensitive, and was inhibited by the removal of external Na+ or by the Na(+)-channel-blocker amiloride (10-44 microM), but K(+)-channel-blockers 4-aminopyridine (0.1-5 mM) and quinine (100 microM) had no effect. The drug induced a sodium dependent, amiloride-sensitive transient inward current reaching its maximum value approx. 20-30 s after the administration of BT and lasting for 6-10 min. This current was activated by depolarization within 25 ms at around -42 mV, its inactivation took about 2 s and its reversal potential was +24 +/- 5 mV. An increase in the intracellular sodium concentration (1.8-3.2 mM) has been observed upon the addition of BT by monitoring the SBFI fluorescence of the dye-loaded cells. It has been shown that whole-cell K+ currents are significantly decreased by BT. The existence of voltage and ligand (BT)-gated sodium channels has been postulated in human lymphocytes. These channels are thought to participate in the initiation of membrane repolarization in human lymphocytes, and thereby influence mitogenic or antigen-induced cell-activation processes.
Tárgyszavak:
Orvostudományok
Elméleti orvostudományok
idegen nyelvű folyóiratközlemény külföldi lapban
Bretylium
Ion channel
Patch-clamp
Human lymphocyte
Megjelenés:
Biochimica et Biophysica Acta (BBA). Molecular Cell Research. - 1137 : 2 (1992), p. 143-147. -
További szerzők:
Krasznai Zoltán (1950-) (biofizikus)
Márián Teréz (1950-) (radiobiológus)
Trón Lajos (1941-) (biofizikus)
Recchioni, Rina
Falasca, Marco
Moroni, Fausto
Pieri, Carlo
Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:
Intézményi repozitóriumban (DEA) tárolt változat
DOI
Szerző által megadott URL
Borító:
Saját polcon:
2.
001-es BibID:
BIBFORM043991
Első szerző:
Pieri, Carlo
Cím:
Bretylium differentiates between distinct signal transducing pathways in human lymphocytes / Pieri, C., Bacso, Z., Recchioni, R., Moroni, F., Balazs, M., Gaspar, R., Damjanovich, S.
Dátum:
1993
ISSN:
0006-291X
Megjegyzések:
The selection of signal transducing pathways of T cells depends on the type of triggers. Antigens, antibodies or lectins induce the T cell receptor-CD3 operated pathway, and IL-2 transmits its activation signal via the IL-2 receptor. It has been demonstrated that bretylium, a quaternary ammonium ion, can significantly inhibit the first pathway at the same dose range that stimulates cell activation through the IL-2 receptor system. In the light of the different complexity of the two pathways at the plasma membrane level, and the non-toxic and reversible behavior of the drug, it is suggested that the bretylium induced sustained membrane hyperpolarization is responsible for the observation. This finding may open new possibilities in studying the mechanism of different signal transducing pathways.
Tárgyszavak:
Orvostudományok
Elméleti orvostudományok
idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:
Biochemical And Biophysical Research Communications. - 190 : 2 (1993), p. 654-659. -
További szerzők:
Bacsó Zsolt (1963-) (biofizikus)
Recchioni, Rina
Moroni, Fausto
Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus)
Gáspár Rezső (1944-) (biofizikus)
Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:
Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:
3.
001-es BibID:
BIBFORM006052
Első szerző:
Pieri, Carlo
Cím:
A sodium channel opener inhibits stimulation of human peripheral blood mononuclear cells / Pieri, C., Recchioni, R., Moroni, F., Marcheselli, F., Falasca, M., Krasznai, Z., Gaspar, R., Matyus, L., Damjanovich, S.
Dátum:
1992
Megjegyzések:
The role of membrane potential changes in T cell activation was studied on human peripheral blood lymphocytes stimulated with phytohemagglutinin. Addition of bretylium tosylate, a sodium channels opener, to PHA treated lymphocytes modified the membrane potential and consequently blocked cell activation in a dose-dependent fashion. BT was non-toxic even in long-term (72 hr) incubations. It was reversibly removable, and the removal restored the stimulatory effect of PHA. 3H-thymidine incorporation was blocked if BT was present during the first 20-24 hr of the mitogenic activation. The later BT was added after PHA, the less inhibition of proliferation was observed. BT hyperpolarized the lymphocytes also in the presence of PHA. BT hindered the depolarizing effect of high extracellular potassium concns. The sustained polarized state of the lymphocytes did not influence the intracellular calcium increase upon PHA treatment. IL-2 and transferrin receptor expression was not hindered by BT during PHA stimulation of lymphocytes. Addition of rIL-2 did not abolish the inhibitory effect of BT. According to cell-cycle analysis BT arrested the majority of the cells in G1 phase. It is suggested that cell activation demands the flexible maintenance of a relatively narrow membrane potential "window". Any sustained and significant hyper-, or depolarization, may dramatically decrease the effectivity of transmembrane signalling.
Tárgyszavak:
Orvostudományok
Elméleti orvostudományok
idegen nyelvű folyóiratközlemény külföldi lapban
analysis
blood
Bretylium Tosylate
Calcium
Cell Cycle
cytology
Dose-Response Relationship,Drug
drug effects
Flow Cytometry
Human
In Vitro
Interleukin-2
Lymphocyte Transformation
Lymphocytes
Membrane Potentials
pharmacology
physiology
Phytohemagglutinins
Potassium
Receptors,Transferrin
Research
Sodium
Sodium Channels
Support,Non-U.S.Gov't
T-Lymphocytes
Megjelenés:
Molecular Immunology. - 29 : 4 (1992), p. 517-524. -
További szerzők:
Recchioni, Rina
Moroni, Fausto
Marcheselli, Fiorella
Falasca, Marco
Krasznai Zoltán (1950-) (biofizikus)
Gáspár Rezső (1944-) (biofizikus)
Mátyus László (1956-) (biofizikus)
Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:
elektronikus változat
Intézményi repozitóriumban (DEA) tárolt változa
Borító:
Saját polcon:
4.
001-es BibID:
BIBFORM004637
Első szerző:
Recchioni, Rina
Cím:
Melatonin increases the intensity of respiratory burst and prevents L-selectin shedding in human neutrophils in vitro / Recchioni, R., Marcheselli, F., Moroni, F., Gaspar, R., Damjanovich, S., Pieri, C.
Dátum:
1998
ISSN:
006-291X
Megjegyzések:
Effects of melatonin priming of neutrophils and subsequent increase of phorbol 12-miristate 13-acetate stimulated respiratory burst were investigated on the modulation of L-selectin shedding and MAC-1 upregulation. Respiratory burst related H2O2 production and adhesion molecule expression were quantified by flow cytometry. Phorbol 12-miristate 13-acetate dose dependence of intracellular oxidation and adhesion molecule expression showed no relationship between respiratory burst intensity and MAC-1 expression or L-selectin shedding. Treatment of cells with 12.5 nM phorbol 12-miristate 13-acetate resulted in less than 20% of the respiratory burst response, however it induced 91.7% of total MAC-1 expression and 62.8% of L-selectin shedding. Melatonin priming experiments showed also no connection between the extent of respiratory burst and MAC-1 expression, however melatonin priming almost completely prevented L-selectin down-regulation elicited by phorbol 12-miristate 13-acetate, without affecting MAC-1 expression. It is suggested that melatonin may inhibit metalloproteases responsible for L-selectin cleavage
Tárgyszavak:
Orvostudományok
Elméleti orvostudományok
idegen nyelvű folyóiratközlemény külföldi lapban
biosynthesis
blood
Dose-Response Relationship,Drug
drug effects
Flow Cytometry
Human
Hydrogen Peroxide
In Vitro
Kinetics
L-Selectin
Macrophage-1 Antigen
Melatonin
Neutrophils
pharmacology
physiology
Research
Respiratory Burst
Rhodamine 123
Support, Non-U.S.Gov't
Tetradecanoylphorbol Acetate
Megjelenés:
Biochemical and Biophysical Research Communications. - 252 : 1 (1998), p. 20-24. -
További szerzők:
Marcheselli, Fiorella
Moroni, Fausto
Gáspár Rezső (1944-) (biofizikus)
Damjanovich Sándor (1936-2017) (biofizikus)
Pieri, Carlo
Internet cím:
elektronikus változat
elektronikus változat
Borító:
Saját polcon:
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