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001-es BibID:	BIBFORM006037
Első szerző:	Gáspár Rezső (biofizikus)
Cím:	Macrophage activation by polymeric nanoparticles of polyalkylcyanoacrylates : activity against intracellular Leishmania donovani associated with hydrogen peroxide production / Gaspar, R., Préat, V., Opperdoes, F. R., Roland, M.
Dátum:	1992
Megjegyzések:	Nanoparticles of polyalkylcyanoacrylates (PACA) can be useful carrier for the targeting of antileishmanial drugs into macrophages and also possess significant antileishmanial activity by themselves. No significant difference in antileishmanial activity could be detected between nanoparticles of five PACAs with differing alkyl side chains, suggesting that the main degradation products of PACA are not involved in their antileishmanial action. The effect of polyisohexylcyanoacrylate (PIHCA) on the induction of the respiratory burst in a macrophage-like cell line (J774G8) was assessed in non-infected macrophages and in macrophages infected with amastigotes of Leishmania donovani infantum, by measuring nitroblue tetrazolium (NBT) reduction and hydrogen peroxide production. Phagocytosis of PIHCA nanoparticles led to a respiratory burst, which was more pronounced in infected than in uninfected macrophages. The production of reactive oxygen intermediates associated with the respiratory burst was inhibited by addition of superoxide dismutase and catalase to the cell suspensions. The addition of catalase to the culture medium together with PIHCA nanoparticles significantly reduced the antileishmanial activity of PIHCA. Moreover PIHCA nanoparticles did not induce interleukin-1 release by macrophages. It is suggested that the antileishmanial action of PIHCA and other PACA nanoparticles results from the activation of respiratory burst in macrophages.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Animal Catalase Cell Line chemistry Culture Media Cyanoacrylates Drug Carriers drug effects drug therapy Hydrogen Peroxide In Vitro Interleukin-1 Leishmania donovani Leishmaniasis Macrophage Activation Macrophages metabolism Mice Nitroblue Tetrazolium parasitology pharmacology Respiratory Burst Support,Non-U.S.Gov't
Megjelenés:	Pharmaceutical Research. - 9 : 6 (1992), p. 782-787. -
További szerzők:	Preat, Véronique Opperdoes, Frederik R. Roland, M.
Internet cím:	elektronikus változat
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001-es BibID:	BIBFORM006035
Első szerző:	Gáspár Rezső (biofizikus)
Cím:	Drug targeting with polyalkylcyanoacrylate nanoparticles : in vitro activity of primaquine-loaded nanoparticles against intracellular Leishmania donovani / Gaspar R., Opperdoes F. R., Preat V., Roland M.
Dátum:	1992
Megjegyzések:	The efficacy of primaquine-loaded polyisohexylcyanoacrylate (PIHCA) nanoparticles was evaluated using J774G8 macrophage-like cells infected with Leishmania donovani: as an in vitro model of visceral leishmaniasis. The in vitro antileishmanial activity of primaquine-loaded nanoparticles showed a 21-fold increase in ED50 compared with free primaquine. Although unloaded PIHCA nanoparticles also exhibited a significant anti-leishmanial effect, the loaded nanoparticles showed a synergistic effect compared with a mixture of unloaded nanoparticles and free primaquine at equivalent concentrations. Primaquine release and isohexanol production were evaluated in a lysosomal fraction; the correlation of both with protein concentration and the rapid drug release indicate the processes are associated with an enzymatic degradation. The results indicate that PIHCA and other polyalkylcyanoacrylates may be useful for targeting drugs at intracellular Leishmania, and that the unloaded carrier itself could be of interest in experimental chemotherapy of leishmaniasis.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban administration & dosage Animal Cell Line Cyanoacrylates Dose-Response Relationship,Drug Drug Carriers drug effects drug therapy In Vitro Leishmania donovani Leishmaniasis Leishmaniasis,Visceral Macrophages Mice Mice,Inbred BALB C parasitology pharmacology Primaquine Support,Non-U.S.Gov't
Megjelenés:	Annals of Tropical Medicine and Parasitology. - 86 : 1 (1992), p. 41-49. -
További szerzők:	Opperdoes, Frederik R. Preat, Véronique Roland, M.
Internet cím:	elektronikus változat
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