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1.

001-es BibID:BIBFORM004855
Első szerző:Bagdány Miklós
Cím:Anuroctoxin, a new scorpion toxin of the alpha-KTx 6 subfamily, is highly selective for Kv1.3 over IKCa1 ion channels of human T lymphocytes / Bagdany, M., Batista, C. V. F., Valdez-Cruz, N. A., Somodi, S., Rodriguez de la Vega, R. C., Licea, A. F., Varga, Z., Gaspar, R., Possani, L. D., Panyi, G.
Dátum:2005
Megjegyzések:The physiological function of T lymphocytes can be modulated selectively by peptide toxins acting on Kv1.3 K(+) channels. Because Kv1.3-specific peptide toxins are considered to have a significant therapeutic potential in the treatment of autoimmune diseases, the discovery of new toxins is highly motivated. Through chromatographic procedures and electrophysiological assays, using patch-clamp methodology, the isolation of a novel peptide named anuroctoxin was accomplished using the venom of the Mexican scorpion Anuroctonus phaiodactylus. It has 35 amino acid residues with a molecular weight of 4082.8, tightly bound by four disulfide bridges whose complete covalent structure was determined. It has a pyroglutamic acid at the N-terminal region and an amidated C-terminal residue. Sequence comparison and phylogenetic clustering analysis classifies anuroctoxin into subfamily 6 of the alpha-KTx scorpion toxins (systematic name, alpha-KTx 6.12). Patch-clamp experiments show that anuroctoxin is a high-affinity blocker of Kv1.3 channels of human T lymphocytes with a K(d) of 0.73 nM, and it does not block the Ca(2+)-activated IKCa1 K(+) channels. These two channels play different but important roles in T-lymphocyte activation. Furthermore, the toxin practically does not inhibit Shaker IR, mKv1.1, and rKv2.1 channels, whereas the affinity of anuroctoxin for hKv1.2 is almost an order of magnitude smaller than for Kv1.3. The pharmacological profile and the selectivity of this new toxin for Kv1.3 over IKCa1 may provide an important tool for the modulation of the immune system, especially in cases in which selective inhibition of Kv1.3 is required.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Animals
antagonists & inhibitors
Autoimmune Diseases
Biophysics
chemistry
drug effects
Human
Humans
Hungary
Immune System
Intermediate-Conductance Calcium-Activated Potassium Channels
Ion Channels
isolation & purification
Kv1.3 Potassium Channel
Lymphocytes
Models,Molecular
Molecular Weight
pharmacology
Phylogeny
Potassium
Potassium Channel Blockers
Potassium Channels
Potassium Channels,Calcium-Activated
Potassium Channels,Voltage-Gated
Research
Scorpion Venoms
Sequence Alignment
Support
T-Lymphocytes
Toxins
Toxins,Biological
Megjelenés:Molecular Pharmacology. - 67 : 4 (2005), p. 1034-1044. -
További szerzők:Batista, Cesar V. F. Valdez-Cruz, Norma A. Somodi Sándor (1977-) (belgyógyász) Rodriguez de la Vega, Ricardo C. Licea, Alexei F. Varga Zoltán (1969-) (biofizikus, szakfordító) Gáspár Rezső (1944-) (biofizikus) Possani, Lourival Domingos Panyi György (1966-) (biofizikus)
Internet cím:elektronikus változat
DOI
elektronikus változat
Borító:

2.

001-es BibID:BIBFORM005176
Első szerző:Corzo, Gerardo
Cím:A selective blocker of Kv1.2 and Kv1.3 potassium channels from the venomof the scorpion Centruroides suffusus suffusus / Corzo G., Papp F., Varga Z., Barraza O., Espino-Solis P. G., Rodríguez de la Vega R. C., Gaspar R., Panyi G., Possani L. D.
Dátum:2008
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Kv1.3 Potassium Channel
Potassium
Potassium Channels
Megjelenés:Biochemical Pharmacology 76 : 9 (2008), p. 1142-1154. -
További szerzők:Papp Ferenc (1979-) (biofizikus) Varga Zoltán (1969-) (biofizikus, szakfordító) Barraza, Omar Espino-Solis, Pavel G. Rodriguez de la Vega, Ricardo C. Gáspár Rezső (1944-) (biofizikus) Panyi György (1966-) (biofizikus) Possani, Lourival Domingos
Internet cím:elektronikus változat
DOI
Borító:

3.

001-es BibID:BIBFORM046293
Első szerző:Gáspár Rezső (biofizikus)
Cím:Effect of acetylcholine on the electrophysiology and proliferative response of human lymphocytes / Gaspar R., Varga Z., Bene L., Marcheselli F., Pieri C., Damjanovich S.
Dátum:1996
ISSN:0006-291X
Megjegyzések:Using the patch-clamp technique, we determined that 1-15 mM extracellular acetylcholine reduced whole-cell n-type K+ currents in human peripheral blood lymphocytes and accelerated their inactivation. The percentage increase in K+ channel inactivation rate and the degree of drug induced block were independent of membrane potential. In flow cytometric membrane potential measurements with the oxonol dye similar doses of acetylcholine depolarized the lymphocyte population. Both acetylcholine induced K+ channel block and depolarization fully developed within 2 minutes. The depolarizing and K+ channel blocking effects of acetylcholine are in concert. [3H]thymidine incorporation experiments proved that the proliferative response of PHA stimulated peripheral blood lymphocytes was decreased by increasing concentrations of acetylcholine in the 1-50 mM range.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biochemical And Biophysical Research Communications. - 226 : 2 (1996), p. 303-308. -
További szerzők:Varga Zoltán (1969-) (biofizikus, szakfordító) Bene László (1963-) (biofizikus) Marcheselli, Fiorella Pieri, Carlo Damjanovich Sándor (1936-2017) (biofizikus)
Pályázati támogatás:T14655
OTKA
T13335
OTKA
6221
OTKA
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:BIBFORM015989
Első szerző:Gáspár Rezső (biofizikus)
Cím:Measurement and analysis of different aspects of potassium currents in human lymphocytes / R. Gáspár, Z. Varga, Gy. Panyi, Z. Krasznai, C. Pieri, S. Damjanovich
Dátum:1998
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
Megjelenés:Signal Transduction. Single cell techniques / eds. Bert van Duijn; Anneke Wiltink. - p. 214-235.
További szerzők:Varga Zoltán (1969-) (biofizikus, szakfordító) Panyi György (1966-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) Pieri, Carlo Damjanovich Sándor (1936-2017) (biofizikus)
Borító:

5.

001-es BibID:BIBFORM004728
Első szerző:Hajdu Péter (biofizikus)
Cím:Cholesterol modifies the gating of Kv1.3 in human T lymphocytes / Péter Hajdú, Zoltán Varga, Carlo Pieri, György Panyi, Rezső Gáspár
Dátum:2003
ISSN:0031-6768
Megjegyzések:The Kv1.3 potassium channel that belongs to the Shaker family of voltage-gated K(+) channels plays a crucial role in the mitogenic response of T cells. Because it spans the cell membrane its function can be influenced by lipid-protein interactions. In order to study the effect of lipid-protein interactions on the functioning of Kv1.3 we manipulated the membrane cholesterol content in T cells mimicking various physiological conditions by means of the oligosaccharide methyl-beta-cyclodextrin (MbetaCD) and its cholesterol-saturated complex (MbetaCD/C). Fluorescence polarization anisotropy and peak current density were used to monitor the efficiency of cholesterol removal (MbetaCD) and loading (MbetaCD/C). Using whole-cell patch-clamp technique we determined the kinetic and steady-state parameters of activation and inactivation of the Kv1.3 currents under different treatment conditions. Upon elevation of cholesterol content by 1 or 1.5 mg/ml MbetaCD/C the rates of both activation and inactivation were slowed. Moreover, the increased cholesterol level in the membrane resulted in a biphasic activation curve. Cholesterol depletion with MbetaCD (0.95 and 1.425 mg/ml) caused no significant changes in the gating characteristics of Kv1.3. The equilibrium between the open and the closed states of the channels was affected by increased cholesterol content, but at the same time steady-state inactivation was unchanged. We argue that manipulation of membrane cholesterol changed both the kinetic properties of Kv1.3 and steady-state parameters of activation by modifying lipid-protein interactions
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
beta-Cyclodextrins
Biophysics
Cell Membrane
Cells
Cholesterol
Cyclodextrins
drug effects
Electric Conductivity
Fluorescence
Fluorescence Polarization
Human
Humans
Hungary
Ion Channel Gating
Kinetics
Kv1.3 Potassium Channel
Lymphocytes
Membrane Microdomains
Membrane Potentials
metabolism
pharmacology
physiology
Potassium
Potassium Channels
Potassium Channels,Voltage-Gated
Research
Support
T-Lymphocytes
Megjelenés:Pflügers Archiv. - 445 : 6 (2003), p. 674-682. -
További szerzők:Varga Zoltán (1969-) (biofizikus, szakfordító) Pieri, Carlo Panyi György (1966-) (biofizikus) Gáspár Rezső (1944-) (biofizikus)
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

6.

001-es BibID:BIBFORM004881
Első szerző:Olamendi-Portugal, Timoteo
Cím:Novel alpha-KTx peptides from the venom of the scorpion Centruroides elegans selectively blockade Kv1.3 over IKCa1 K+ channels of T cells / Olamendi-Portugal, T., Somodi, S., Fernandez, J. A., Zamudio, F. Z., Becerril, B., Varga, Z., Panyi, G., Gaspar, R., Possani, L. D.
Dátum:2005
ISSN:0041-0101
Megjegyzések:From the venom of the Mexican scorpion Centruroides elegans Thorell five peptides were isolated to homogeneity by chromatographic procedures and their full amino acid sequence was determined by automatic Edman degradation. They all belong to the Noxiustoxin subfamily of scorpion toxins and were given the systematic names alpha-KTx 2.8 to 2.12, with trivial names Ce1 to Ce5, respectively. They have 39 amino acid residues, except for Ce3 which has only 38, but all of them have three disulfide bridges, and have molecular weights of 4255, 4267, 4249, 4295 and 4255 atomic mass units, respectively for Ce1 to Ce5. The C-terminal residues of Ce2, Ce4 and Ce5 were found to be amidated. The electrophysiological assay (whole-cell patch-clamp) showed that out of the five peptides, Ce1 (alpha-KTx 2.8), Ce2 (alpha-KTX2.9) and Ce4 (alpha-KTx 2.11) were effective blockers of Kv1.3 channels of human T lymphocytes, whereas these peptides did not inhibit the Ca2+-activated K+ channels (IKCa1) of the same cells. The equilibrium dissociation constants of these peptides for Kv1.3 were 0.70, 0.25 and 0.98nM for Ce1, Ce2 and Ce4, respectively. Furthermore, toxins Ce1, Ce2 and Ce4 practically did not inhibit the related voltage gated Shaker K+ channels, and rKv2.1 channels of the Shab family. The high affinity blockage of Kv1.3 channels by these peptides and their selectivity for Kv1.3 over IKCa1 may have significance in the development of novel tools for suppressing the function of those T cell subsets whose proliferation critically depends on the activity of Kv1.3 channels.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Amino Acid Sequence
Animals
antagonists & inhibitors
Bayes Theorem
Cell Line
Cells
chemistry
Chromatography,High Pressure Liquid
Comparative Study
Electrophysiology
Enzyme-Linked Immunosorbent Assay
genetics
Human
Humans
Intermediate-Conductance Calcium-Activated Potassium Channels
Kv1.3 Potassium Channel
Lymphocytes
Mass Spectrometry
metabolism
Mexico
Models,Genetic
Molecular Sequence Data
Molecular Weight
Organophosphorus Compounds
Peptides
Phylogeny
Potassium
Potassium Channels
Research
Scorpion Venoms
Scorpions
Sequence Analysis,Protein
Support
T-Lymphocytes
toxicity
Toxins
Megjelenés:Toxicon. - 46 : 4 (2005), p. 418-429. -
További szerzők:Somodi Sándor (1977-) (belgyógyász) Fernandez, Juan Antonio Zamudio, Fernando Z. Becerril, Baltazar Varga Zoltán (1969-) (biofizikus, szakfordító) Panyi György (1966-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Possani, Lourival Domingos
Internet cím:elektronikus változat
DOI
Borító:

7.

001-es BibID:BIBFORM004849
035-os BibID:WOS:000188796800035
Első szerző:Panyi György (biofizikus)
Cím:Kv1.3 potassium channels are localized in the immunological synapse formed between cytotoxic and target cells / Panyi, G., Vamosi, G., Bacso, Z., Bodnar, A., Bagdany, M., Varga, Z., Gaspar, R., Matyus, L., Damjanovich, S.,
Dátum:2004
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Kv1.3 Potassium Channel
Potassium
Potassium Channels
egyetemen (Magyarországon) készült közlemény
Megjelenés:Proceedings of the National Academy of Sciences of the United States of America. - 101 : 5 (2004), p. 1285-1290. -
További szerzők:Vámosi György (1967-) (biofizikus) Bacsó Zsolt (1963-) (biofizikus) Dóczy-Bodnár Andrea (1970-) (biofizikus) Bagdány Miklós Varga Zoltán (1969-) (biofizikus, szakfordító) Gáspár Rezső (1944-) (biofizikus) Mátyus László (1956-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
DOI
Borító:

8.

001-es BibID:BIBFORM004715
Első szerző:Panyi György (biofizikus)
Cím:Pharmacological effects of melatonin on ion channels / Panyi, G., Somodi, S., Varga, Z., Hajdu, P., Pieri, C., Pandi-Perumal, S. R., Damjanovich, S., Gaspar, R., Hardar, C., Singaravel, M., Maitra, S. K.
Dátum:2002
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
Ion Channels
Melatonin
Megjelenés:Treatise on Pineal Gland and Melatonin. - p. 489-506.
További szerzők:Somodi Sándor (1977-) (belgyógyász) Varga Zoltán (1969-) (biofizikus, szakfordító) Hajdu Péter (1975-) (biofizikus) Pieri, Carlo Pandi-Perumal, Seithikurippu R. Damjanovich Sándor (1936-2017) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Hardar, C. Singaravel, M. Maitra, S. K.
Borító:

9.

001-es BibID:BIBFORM005093
Első szerző:Panyi György (biofizikus)
Cím:K+ channel blockers : novel tools to inhibit T cell activation leading to specific immunosuppression / Panyi, G., Possani, L. D., Rodriguez de la Vega R. C., Gaspar, R., Varga, Z.
Dátum:2006
ISSN:381-6128 (Print)
Megjegyzések:During the last two decades since the identification and characterization of T cell potassium channels great advances have been made in the understanding of the role of these channels in T cell functions, especially in antigen-induced activation. Their limited tissue distribution and the recent discovery that different T cell subtypes carrying out distinct immune functions show specific expression levels of these channels have made T cell potassium channels attractive targets for immunomodulatory drugs. Many toxins of various animal species and a structurally diverse array of small molecules inhibiting these channels with varying affinity and selectivity were found and their successful use in immunosuppression in vivo was also demonstrated. Better understanding of the topological differences between potassium channel pores, detailed knowledge of toxin and small-molecule structures and the identification of the binding sites of blocking compounds make it possible to improve the selectivity and affinity of the lead compounds by introducing modifications based on structural information. In this review the basic properties and physiological roles of the voltage-gated Kv1.3 and the Ca2+-activated IKCa1 potassium channels are discussed along with an overview of compounds inhibiting these channels and approaches aiming at producing more efficient modulators of immune functions for the treatment of diseases like sclerosis multiplex and type I diabetes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Amino Acid Sequence
Animal
Animals
Binding Sites
Biophysics
chemistry
Cnidarian Venoms
Drug Design
drug effects
Humans
Hungary
immunology
Immunosuppression
Immunosuppressive Agents
Intermediate-Conductance Calcium-Activated Potassium Channels
Ion Channel Gating
Kv1.3 Potassium Channel
Lymphocyte Activation
metabolism
Models, Molecular
Molecular Sequence Data
Molecular Structure
pharmacology
Potassium
Potassium Channel Blockers
Potassium Channels
Protein Conformation
Pyrazoles
Quinolines
Research
Structure-Activity Relationship
Support
T-Lymphocytes
Toxins
Megjelenés:Current Pharmaceutical Design. - 12 : 18 (2006), p. 2199-2220. -
További szerzők:Possani, Lourival Domingos Rodriguez de la Vega, Ricardo C. Gáspár Rezső (1944-) (biofizikus) Varga Zoltán (1969-) (biofizikus, szakfordító)
Borító:

10.

001-es BibID:BIBFORM004842
Első szerző:Panyi György (biofizikus)
Cím:Ion channels and lymphocyte activation / Panyi, G., Varga, Z., Gaspar, R.
Dátum:2004
Megjegyzések:The ion channels expressed by T lymphocytes play key roles in the control of the membrane potential and calcium signaling, thereby affecting signal transduction pathways that lead to the activation of these cells following antigenic stimulation. Disruption of these pathways can attenuate or prevent the response of T-cells to antigenic challenge resulting in immune suppression. Studies using ion channel blockers of high affinity and specificity have shown that this interference can be achieved at the level of ion channels. Suppression of immune functions by channel blockers has been demonstrated in vitro and in vivo. New information about the molecular structure of ion channels facilitates the design of more potent and more specific inhibitors. Thus, T-cell ion channels are likely to serve as targets for immunomodulatory drugs in the near future. Here, the biophysical properties, tissue distribution, regulation of expression, molecular pharmacology and role in T-cell activation of the voltage-gated Kv1.3 and the Ca(2+)-activated IKCa1 potassium channels and those of the Ca(+) release-activated Ca(2+) (CRAC) channel are reviewed.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animals
Biophysics
Calcium
Calcium Channel Blockers
Calcium Channels
Calcium Signaling
Cells
Humans
Hungary
immunology
In Vitro
Ion Channels
Lymphocyte Activation
Lymphocytes
Membrane Potentials
Molecular Structure
pharmacology
physiology
Potassium
Potassium Channel Blockers
Potassium Channels
Research
Signal Transduction
Support
T-Lymphocytes
Tissue Distribution
Megjelenés:Immunology Letters. - 92 : 1-2 (2004), p. 55-66. -
További szerzők:Varga Zoltán (1969-) (biofizikus, szakfordító) Gáspár Rezső (1944-) (biofizikus)
Internet cím:elektronikus változat
DOI
Borító:

11.

001-es BibID:BIBFORM010358
Első szerző:Papp Ferenc (biofizikus)
Cím:Tst26, a novel peptide blocker of Kv1.2 and Kv1.3 channels from the venom of Tityus stigmurus / Papp, F., Batista, C. V. F., Varga, Z., Herceg, M., Roman-Gonzalez, S. A., Gaspar, R., Possani, L. D., Panyi, G.
Dátum:2009
ISSN:0041-0101 (Print)
Megjegyzések:Using high-performance liquid chromatography Tst26, a novel potassium channel blocker peptide, was purified from the venom of the Brazilian scorpion Tityus stigmurus. its primary structure was determined by means of automatic Edman degradation and mass spectrometry analysis. The peptide is composed of 37 amino acid residues and tightly folded through three disulfide bridges, similar to other K+ channel blocking peptides purified from scorpion venoms. It contains the "essential dyad" for K+ channel recognition comprised of a lysine at position 27 and a tyrosine at position 36. Electrophysiological assays revealed that Tst26 blocked hKv1.2 and hKv1.3 channels with high affinity (K-d = 1.9 nM and 10.7 nM, respectively) while it did not affect several other ion channels (mKv1.1, hKv1.4, hKv1.5, hERG, hIKCa1, hBK, hNav1.5) tested at 10 nM concentration. The voltage-dependent steady-state parameters of K+ channel gating were unaffected by the toxin in both channels, but due to the fast association and dissociation kinetics Tst26 slowed the rate of inactivation of Kv1.3 channels. Based on the primary structure, the systematic nomenclature proposed for this peptide is alpha-KTx 4.6.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Chromatography
Ion Channels
Kinetics
Lysine
Mass Spectrometry
Peptides
Potassium
Scorpion Venoms
Spectrometry
Tyrosine
Megjelenés:Toxicon. - 54 : 4 (2009), p. 379-389. -
További szerzők:Batista, Cesar V. F. Varga Zoltán (1969-) (biofizikus, szakfordító) Herceg Mónika (biofizikus) Roman-Gonzalez, Sergio A. Gáspár Rezső (1944-) (biofizikus) Possani, Lourival Domingos Panyi György (1966-) (biofizikus)
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

12.

001-es BibID:BIBFORM005189
Első szerző:Papp Ferenc (biofizikus)
Cím:CRAC channels in developing human dendritic cells / Papp, F., Hajdu, P., Varga, Z., Zsiros, E., Ludanyi, K., Gaspar, R., Rajnavolgyi, E., Panyi, Gy.
Dátum:2008
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Human
Dendritic Cells
Cells
Megjelenés:Biophysical Journal. - 94 : Suppl (2008), p. B290 -
További szerzők:Hajdu Péter (1975-) (biofizikus) Varga Zoltán (1969-) (biofizikus, szakfordító) Zsíros Emese (1980-) (orvos) Ludányi Katalin (1975-) (immunológus) Gáspár Rezső (1944-) (biofizikus) Rajnavölgyi Éva (1950-) (immunológus) Panyi György (1966-) (biofizikus)
Internet cím:elektronikus változat
Borító:
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