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001-es BibID:	BIBFORM042927
035-os BibID:	PMID:23466426
Első szerző:	Palicz Zoltán (orvos)
Cím:	In vivo application of a small molecular weight antifungal protein of Penicillium chrysogenum (PAF) / Zoltán Palicz, Ágnes Jenes, Tamás Gáll, Kornél Miszti-Blasius, Sándor Kollár, Ilona Kovács, Miklós Emri, Teréz Márián, Éva Leiter, István Pócsi, Éva Csősz, Gergő Kalló, Csaba Hegedűs, László Virág, László Csernoch, Péter Szentesi
Dátum:	2013
ISSN:	0041-008X
Megjegyzések:	The antifungal protein of Penicillium chrysogenum (PAF) inhibits the growth of important pathogenic filamentous fungi, including members of the Aspergillus family and some dermatophytes. Furthermore, PAF was proven to have no toxic effects on mammalian cells in vitro. To prove that PAF could be safely used in therapy, experiments were carried out to investigate its in vivo effects. Adult mice were inoculated with PAF intranasally in different concentrations, up to 2700g·kg(-1) daily, for 2weeks. Even at the highest concentration - a concentration highly toxic in vitro for all affected molds - used, animals neither died due to the treatment nor were any side effects observed. Histological examinations did not find pathological reactions in the liver, in the kidney, and in the lungs. Mass spectrometry confirmed that a measurable amount of PAF was accumulated in the lungs after the treatment. Lung tissue extracts from PAF treated mice exerted significant antifungal activity. Small-animal positron emission tomography revealed that neither the application of physiological saline nor that of PAF induced any inflammation while the positive control lipopolysaccharide did. The effect of the drug on the skin was examined in an irritative dermatitis model where the change in the thickness of the ears following PAF application was found to be the same as in control and significantly less than when treated with phorbol-12-myristate-13-acetate used as positive control. Since no toxic effects of PAF were found in intranasal application, our result is the first step for introducing PAF as potential antifungal drug in therapy.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Molekuláris Medicina
Megjelenés:	Toxicology and Applied Pharmacology 269 : 1 (2013), p. 8-16. -
További szerzők:	Jenes Ágnes (1980-) (élettanász) Gáll Tamás (1982-) (molekuláris biológus, mikrobiológus) Miszti-Blasius Kornél (1977-) (laboratóriumi szakorvos) Kollár Sándor (1949-) (sebész) Kovács Ilona (1965-) (patológus) Emri Miklós (1962-) (fizikus) Márián Teréz (1950-) (radiobiológus) Leiter Éva (1976-) (biológus) Pócsi István (1961-) (vegyész) Csősz Éva (1977-) (biokémikus, molekuláris biológus) Kalló Gergő (1989-) (molekuláris biológus) Hegedűs Csaba (1980-) (biokémikus, molekuláris biológus) Virág László (1965-) (biokémikus, sejtbiológus, farmakológus) Csernoch László (1961-) (élettanász) Szentesi Péter (1967-) (élettanász)
Pályázati támogatás:	TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Oxidatív stressz és ADP-riboziláció kapcsolatának vizsgálata TÁMOP-4.2.2/B-10/1-2010-0024 TÁMOP Molekuláris Orvostudomány Doktori Iskola TÁMOP-4.2.2.A-11/1/KONV-2012-0025 TÁMOP
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001-es BibID:	BIBFORM027492
Első szerző:	Trencsényi György (biológus, biokémikus, molekuláris biológus)
Cím:	Renal capsule-parathymic lymph node complex : a new in vivo metastatic model in rats / Gyorgy Trencsenyi, Pal Kertai, Fruzsina Bako, Janos Hunyadi, Terez Marian, Zoltan Hargitai, Istvan Pocsi, Edit Muranyi, Laszlo Hornyak, Gaspar Banfalvi
Dátum:	2009
ISSN:	0250-7005
Megjegyzések:	BACKGROUND: The ultimate cause of cancer death is, in most cases, the appearance of metastases. The aim of the present study was to contribute to animal experimental investigations of metastatic tumor development. MATERIALS AND METHODS: Rat hepatocarcinoma (He/De), mesoblastic nephroma (Ne/De) cells, and in other cases tumor-bearing lymph nodes were transplanted under the renal capsule of F344 rats. Metastatic potential of tumor cells was examined by whole body autoradiography and phosphor image analysis. The organ distribution of cells was also investigated. RESULTS: Transplanted tumor cells resulted in metastases in the parathymic lymph nodes. Implanted India ink also demonstrated connection between the lymphatic vessels of the renal capsule and the parathymic lymph nodes. The metastatic potential was independent of the primary tumor growth rate. CONCLUSION: The renal capsule-parathymic lymph node complex seems to be suitable for the isolated in vivo examination of metastatic development and for the detailed analysis of secondary tumors.
Tárgyszavak:	Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban Hepatocarcinoma mesoblastic nephroma metastasis parathymic lymph node autoradiography
Megjelenés:	Anticancer Research. - 29 : 6 (2009), p. 2121-2126. -
További szerzők:	Kertai Pál (1927-2016) (népegészségügyi szakember) Bakó Fruzsina Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Márián Teréz (1950-) (radiobiológus) Hargitai Zoltán Pócsi István (1961-) (vegyész) Murányi Edit Hornyák László Bánfalvi Gáspár (1943-) (sejtbiológus, gyógyszerész)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM015778
Első szerző:	Trencsényi György (biológus, biokémikus, molekuláris biológus)
Cím:	Renal capsule parathymic lymph node complex : imaging of a new in vivo metastatic model in rats with 18F-FDG / Gy. Trencsenyi, P. Kertai , F. Bako, J. Hunyadi, T. Marian, S. A. Kis, G. Opposits, M. Emri, I. Pocsi, G. Banfalvi
Dátum:	2009
Megjegyzések:	Background: In our experiments, we wished to prove - using mini-PET, autora- diography and organ distribution examinations - that rat hepatocarcinoma (He/De), and mesoblastic nephroma (Ne/De) cells give metastases to the parathy- mic lymph nodes. Material and methods: 106 He/De or Ne/De cells were placed under the capsule of the left kidney. Two weeks after implantation, control and tumor-bearing rats were anesthetized and a radioligand, 18FDG (15.0 MBq), was injected i.v. For autorad- iography, 60 ?m thick cryostate sections (Leica cryomacrotome) were cut and ex- posed to phosphor imaging plates. Results were expressed in intensity pixel units. For organ-distribution, different tissues were removed and their activities were mea- sured. The radioactivity of the samples was used to determine the differential ab- sorption ratio (DAR). Results: By taking the pixel density of resting striated muscle as one unit, the rela- tive pixel densities were in decreasing order: 14.23 in He/De tumor, 10.82 in par- athymic lymph nodes, 5.36 in kidney, 2.35 in blood and 1.57 in liver. In the case of the Ne/De, the results were the same. The DAR values also showed that the majority of the radioactivity was accumulated in the tumors and in the parathymic lymph nodes. Conclusions: From the autoradiographic, phosphor image and tissue distribution experiments, we concluded that He/De and Ne/De tumors grown under the cap- sule of kidney represent a significant metastatic burden manifested primarily in parathymic lymph nodes. The renal capsule and parathymic lymph node complex seems to be suitable for the isolated in vivo examination of metastatic development and for the detailed analysis of secondary tumors.
Tárgyszavak:	Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:	Nuclear Medicine Review 12 : 1 (2009), p. 39. -
További szerzők:	Kertai Pál (1927-2016) (népegészségügyi szakember) Bakó Ferenc (biológus) Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Márián Teréz (1950-) (radiobiológus) Kis Sándor Attila (1973-) (fizikus) Opposits Gábor (1974-) (fizikus, szoftver fejlesztő) Emri Miklós (1962-) (fizikus) Pócsi István (1961-) (vegyész) Bánfalvi Gáspár (1943-) (sejtbiológus, gyógyszerész)
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM013303
Első szerző:	Trencsényi György (biológus, biokémikus, molekuláris biológus)
Cím:	Comparison of the tumorigenic potential of liver and kidney tumors induced by N-nitrosodimethylamine / Trencsényi György, Juhász Tamás, Bakó Fruzsina, Márián Teréz, Pócsi István, Kertai Pál, Hunyadi János, Bánfalvi Gáspár
Dátum:	2010
Megjegyzések:	The aim of the study was to determine the tumorigenic potential of two cell lines established from N-nitrosodimethylamine induced rat hepatocarcinoma (HeDe) and mesenchymal renal tumors (NeDe). The basis of the distinction is that human cancers are known to overexpress facilitative GLUT transporters and TGF-beta1 protein. These proteins are linked to the increased metabolic energy consumption indicating uncontrolled growth and proliferation. We have assayed not only the expression of GLUT-1, GLUT-3 and TGF-beta1 proteins, but also the uptake of 2-fluoro-[18F]-2-deoxy-D-glucose (18FDG), a tracer for cancer diagnosis. Western blot analysis and whole body autoradiography were used to measure the 18FDG uptake of tumor cells. Elevated 18FDG uptake was measured in both tumor cell lines. Whole body autoradiography provided evidence that the uptake of 18FDG was lower in the necrotic inner part than in the more vascularized outer parts of primary hepatocarcinoma and mesenchymal renal tumors. GLUT-1 overexpression in hepatocarcinoma tumor, and high levels of GLUT-3 were found in the NeDe cell line and in the mesenchymal renal tumor. TGF-beta-1 was overexpressed in hepatocarcinoma and mesenchymal renal tumors. In vitro and in vivo parameters support the view that the tumorigenic potential of cancer cells cannot be determined by the expression of a single parameter such as the expression of either GLUT-1, GLUT-3 or 18FDG uptake. Besides the tumorigenic potential of the hepatocarcinoma, the high metabolic activity of the renal tumor indicated by its 18FDG uptake, GLUT-3 and TGF-beta1 expression, the mesenchymal renal tumor induced by N-nitroso-dimethylamine is not a benign, but an an aggressive renal carcinoma.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Chemical induction Primary tumor Tumor cell lines Gene expression GLUT transporters 18FDG uptake Whole body autoradiography
Megjelenés:	Histology and Histopathology 25 : 3 (2010), p. 309-320. -
További szerzők:	Juhász Tamás (1976-) (biológus, orvosbiológus) Bakó Fruzsina Márián Teréz (1950-) (radiobiológus) Pócsi István (1961-) (vegyész) Kertai Pál (1927-2016) (népegészségügyi szakember) Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Bánfalvi Gáspár (1943-) (sejtbiológus, gyógyszerész)
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