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001-es BibID:	BIBFORM009362
Első szerző:	Demeter Zita (biológia-kémia szakos tanár)
Cím:	Somatic embryogenesis and regeneration from shoot primordia of Crocus heuffelianus / Zita Demeter, Gyula Surányi, V. Attila Molnár, Gábor Sramkó, Dániel Beyer, Zoltán Kónya, Gábor Vasas, Márta M-Hamvas, Csaba Máthé
Dátum:	2010
ISSN:	0167-6857 1573-5044
Megjegyzések:	Crocus heuffelianus belongs to the C. vernus(Iridaceae) species aggregate. In the Carpathian Basin andparticularly in Hungary it is considered an endangered spe-cies. Therefore our aim was to establish a tissue culturesystem with potential of germplasm preservation of thistaxon. For in vitro culture experiments, shoot primordia fromcorms were the most suitable. We induced an embryogeniccallus line from those explants on basal Murashige-Skoog(MS) medium supplemented with Gamborg's vitamins, 2%(w/v) sucrose, 10 mg l-1(53.7 lM) a-naphthaleneaceticacid (NAA) and 1 mg l-1(4.44 lM) 6-benzyladenine (BA).Globular stage embryos developed on this medium andseveral culture conditions were used in an attempt to obtainmature embryos and plant regeneration. Firstly a decrease ofauxin/cytokinin concentration and ratio, then secondly adecrease in the strength of culture medium and the concen-tration of carbon source was used, which was effective inembryogenesis and the production of plants. Regenerationmedium used in the second step was fourfold diluted MSmedium and Gamborg's vitamins supplemented with 1%(w/v) sucrose, 0.05 mg l-1(0.26 lM) NAA and 0.5 mg l-1(2.22 lM) BA, with a 14/10 h photoperiod. Under theseconditions we could detect all the stages of somatic embryodevelopment characteristic for Iridaceae. This is the?rst report demonstrating the production of stable tissueculture of C. heuffelianus with potential use in germplasmpreservation via plant regeneration. This study could alsocontribute to a better understanding of somatic embryogen-esis in the Crocus genus.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Crocus heuffelianus Mature somatic embryo Plant regeneration Germplasm preservation
Megjelenés:	Plant Cell Tissue and Organ Culture. - 100 (2010), p. 349-353. -
További szerzők:	Surányi Gyula (1957-) (biológus) Molnár V. Attila (1969-) (biológus, botanikus) Sramkó Gábor (1981-) (biológus) Beyer Dániel (1982-) (molekuláris biológus) Kónya Zoltán (1986-) (molekuláris biológus, biokémikus) Vasas Gábor (1975-) (biológus-vegyész) Mikóné Hamvas Márta (1963-) (biológus) Máthé Csaba (1966-) (biológus)
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001-es BibID:	BIBFORM030845
Első szerző:	Fernández, José-Antonio
Cím:	The World Saffron and Crocus collection : strategies for establishment, management, characterisation and utilisation / José-Antonio Fernández, Omar Santana, José-Luis Guardiola, Rosa-Victoria Molina, Pat Heslop-Harrison, George Borbely, Ferdinando Branca, Sergio Argento, Eleni Maloupa, Thierry Talou, Jean-Marie Thiercelin, Khalil Gasimov, Hasan Vurdu, Marta Roldán, Marcela Santaella, Enrique Sanchýs, Amparo Garcýa-Luis, Gyula Suranyi, Attila Molnár, Gabor Sramko, Gergely Gulyas, Luckacs Balazs, Orsolya Horvat, Marýa-Fernanda Rodrýguez, Raúl Sánchez-Vioque, Miguel-Ángel Escolano, José-Vicente Reina, Nikos Krigas, Teresa Pastor, Begoña Renau-Morata, Christine Raynaud, Oruc Ibadli, Moschos Polissiou, Maria Z. Tsimidou, Athanasios Tsaftaris, Mahmoud Sharaf-Eldin, Joaquin Medina, Theophanis Constantinidis, Theophanis Karamplianis, Marcelino De-Los-Mozos-Pascual
Dátum:	2010
ISSN:	0925-9864
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Genetic Resources And Crop Evolution. - 58 : 1 (2010), p. 125-137. -
További szerzők:	Santana, Omar Guardiola, José-Luis Molina, Rosa-Victoria Heslop-Harrison, Pat Borbély György (1943-) (biológus) Branca, Ferdinando Argento, Sergio Maloupa, Eleni Talou, Thierry Thiercelin, Jean-Marie Gasimov, Khalil Vurdu, Hasan Roldán, Marta Santaella, Marcela Sanchís, Enrique García-Luis, Amparo Surányi Gyula (1957-) (biológus) Molnár V. Attila (1969-) (biológus, botanikus) Sramkó Gábor (1981-) (biológus) Gulyás Gergely Balázs Luckacs Horvát Orsolya Rodríguez, María-Fernanda Sánchez-Vioque, Raúl Escolano, Miguel-Ángel Reina, José-Vicente Krigas, Nikos Pastor, Teresa Renau-Morata, Begoña Raynaud, Christine Ibadli, Oruc Polissiou, Moschos Tsimidou, Maria Z. Tsaftaris, Athanasios Sharaf-Eldin, Mahmoud Medina, Joaquin Constantinidis, Theophanis Karamplianis, Theophanis De-Los-Mozos-Pascual, Marcelino
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001-es BibID:	BIBFORM062727
Első szerző:	Mosolygó Ágnes (biológus)
Cím:	Molecular genetic evidence for allotetraploid hybrid speciation in the genus Crocus L. (Iridaceae) / Ágnes Mosolygó-L., Gábor Sramkó, Sándor Barabás, Levente Czeglédi, András Jávor, Attila Molnár V., Gyula Surányi
Dátum:	2016
ISSN:	1179-3155 1179-3163
Megjegyzések:	Although dysploidy and polyploidisation events are known to be important drivers in the evolutionary history of the genus Crocus, only a few examples of natural hybrid origins have so far been documented. Here, we describe the phylogenetic affinities of five taxa in the Crocus vernus species complex from the Carpathian Basin in Central Europe. Genetic variability was evaluated using chloroplast DNA sequences of the accD-psaI intergenic spacer and amplified fragment length polymorphism (AFLP) in combination with karyological observations. Genetic diversity and differentiation of the seven investigated Crocus species were also evaluated using AFLP data. We created a phylogenetic hypothesis using both sequences and AFLP fingerprinting data under maximum parsimony (MP). AFLPs were also analysed by means of multivariate statistics using principal coordinate (PCoA) analysis and Bayesian clustering (BC) to test for hybridity. Both the AFLP and plastid phylogenetic trees separated the taxa into two groups: (1) a ♭Balkan' clade, and; (2) an ♭Adriatic' clade. The Balkan clade contained Crocus heuffelianus and Crocus vittatus samples from Transylvania as well as Crocus tommasinianus samples from Hungary; the Adriatic clade included Crocus vittatus and Crocus vernus samples from Hungary and Croatia as well as Crocus neapolitanus from Italy. A hard incongruence was found in the placement of the Slovakian Crocus scepusiensis which clustered inside the Balkan clade on our plastid tree, while it fell in the Adriatic clade on the AFLP tree. The same populations occupied intermediate position on the PCoA plot. The BC analysis assigned all Crocus scepusiensis specimens as F1 hybrids, while Crocus vernus and Crocus heuffelianus were assigned as parental species. Together with our cytological investigation that determined a 2n=18 chromosome number of Crocus scepusiensis, we conclude an allopolyploid hybrid origin for this Northern Carpathian taxon as the result of a cross between members of the 2n=10 Balkan clade (e.g., Crocus heuffelianus from Transylvania) and the 2n=8 Adriatic clade (Crocus vernus). A similar origin is postulated for the North Balkan Crocus vittatus (2n=18), which was clustered as an F1 hybrid in the BC analysis; thus, parallel evolution may have taken place in the northern and southern part of the Carpathian Basin beginning from the same parental species but leading to different allopolyploid derivatives.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban AFLP-fingerprinting Carpathian Basin hybridisation incongruence parallelism polyploidisation
Megjelenés:	Phytotaxa. - 258 : 2 (2016), p. 121-136. -
További szerzők:	Sramkó Gábor (1981-) (biológus) Barabás Sándor (biológus) Czeglédi Levente (1977-) (agrármérnök) Jávor András (1952-) (agrármérnök) Molnár V. Attila (1969-) (biológus, botanikus) Surányi Gyula (1957-) (biológus)
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