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001-es BibID:	BIBFORM085355
Első szerző:	Gyémant N.
Cím:	In vitro search for synergy between flavonoids and epirubicin on multidrug-resistant cancer cells / N. Gyémant, M. Tanaka, S. Antus, J. Hohmann, O. Csuka, L. Mandoky, J. Molnar
Dátum:	2005
ISSN:	0258-851X
Megjegyzések:	The drug accumulation of a human multidrug resistance 1 (mdr1) gene-transfected mouse lymphoma cell line and a multidrug resistance protein (MRP)-expressing human breast cancer cell line MDA-MB-231 was compared in the presence of sixteen flavonoids and five isoflavonoids. The expression of the 170-kDa P-glycoprotein (P-gp) (MDR1) and 190-kDa multidrug resistance protein (MRP) in both cell lines was confirmed by immunocytochemistry. The rhodamine 123 accumulation of the P-glycoprotein (P-gp)-expressing cells increased up to 46.4, while 2',7'-bis(2-carboxyethyl)-5(6)carboxy-fluorescein acetoxymethyl ester (BCECF-AM) accumulation of the MRP-expressing cells increased up to 1.6, in fluorescence activity ratio (FAR). Major P-gp-mediated efflux pump modifiers are formononetin, amorphigenin, rotenone and chrysin, while MRP-mediated efflux pump modifiers are formononetin, afrormosin, robinin, kaempferol and epigallocatechin. In antiproliferative assay, afrormosin, amorphigenin, chrysin and rotenone exhibited the strongest antiproliferative effects in L5178 (max. ID50: 19.70) and MDA-MB-231 cell lines (max. ID50: 55.47). In a checkerboard microplate method in vitro, furthermore, the most effective multidrug resistance (MDR) resistance modifiers, amorphigenin, formononetin, rotenone and chrysin, were assayed for their antiproliferative effects in combination with epirubicin. Rotenone and afrormosin showed additive effects. Chrysin and amorphigenin on the mouse lymphoma cell line and formononetin on the MDA-MB-231 cell line synergistically enhanced the effect of epirubicin.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Multidrug resistance P-glycoprotein flavonoids isoflavonoids.
Megjelenés:	In Vivo. - 19 : 2 (2005), p. 367-374. -
További szerzők:	Tanaka, Mana Antus Sándor (1944-2022) (vegyészmérnök) Hohmann Judit Csuka Orsolya Mándoky László Molnár J.
Pályázati támogatás:	T034250 OTKA
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM085356
Első szerző:	Ugocsai Katalin
Cím:	Effects of selected flavonoids and carotenoids on drug accumulation and apoptosis induction in multidrug-resistant colon cancer cells expressing MDR1/LRP / Katalin Ugocsai, Andreas Varga, Péter Molnar, Sandor Antus, Joseph Molnar
Dátum:	2005
ISSN:	0258-851X
Megjegyzések:	The effects of various flavonoids and carotenoids on Rhodamine 123 accumulation in multidrug-resistant Colo 320 human colon cancer cells expressing MDR1/LRP were studied. The Colo 205 cell line was used as a drug-sensitive control. Rotenon, Catechin, Neohesperidin, Naringin, Robinin, Phloridzin, Robinetin, Dihydrobinetin, Dihydrofisetin, Kampferol, Dihidroquercetin, Sakuranin and Sakuranetin were tested on Colo 320 cells: only Rotenon was found to be effective as regards multidrug resistance (MDR) reversal, while a majority of the flavonoids, such as Catechin, Neohesperidin, Naringin, Robinin, Phloridzin, Dihydrobinetin and Sakuranetin, had only marginal effects on Rhodamine 123 accumulation. The tested carotenoids (?-Cryptoxanthin, Luteoxanthin, Anteroxanthin, Violeoxanthin, Apple peel fetoxanthin, Lutein, Violaxanthin and Neoxanthin) were able to increase Rhodamine 123 accumulation in Colo 320 cells. Verapamil was applied as a resistance-modifying positive control. The levels of apoptosis induction in drug-resistant and sensitive cell lines were also compared. The results indicated that the tested flavonoids were weak apoptosis inducers on MDR and parent cells, without significant differences. A majority of the carotenoids induced only early apoptosis, but apoptosis and cell death were not induced in MDR colon cancer cells.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Colon cancer cell lines carotenoids flavonoids multidrug resistance MDR1/LRP apoptosis
Megjelenés:	In Vivo. - 19 : 2 (2005), p. 433-438. -
További szerzők:	Varga Andreas Molnár Péter Antus Sándor (1944-2022) (vegyészmérnök) Molnar Joseph
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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