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001-es BibID:	BIBFORM105734
035-os BibID:	(WOS)000319074600029 (Scopus)84878011489
Első szerző:	Siesto, Gabriella
Cím:	Polymorphism detection among wild Saccharomyces cerevisiae strains of different wine origin / Gabriella Siesto, Angela Capece, Matthias Sipiczki, Hajnalka Csoma, Patrizia Romano
Dátum:	2013
ISSN:	1590-4261 1869-2044
Megjegyzések:	In this study, wild Saccharomyces cerevisiae strains, isolated from spontaneously fermenting grapes of different varieties and origins, were submitted to genetic analysis using different molecular techniques, such as amplification of genes coding for cell wall proteins and containing minisatellite-like sequences, karyotyping, mtDNA-RFLP, and analysis of the delta region. The lowest discriminative power was obtained by minisatellites analysis, in particular the amplification of AGA1 genes. Karyotyping and mtDNA-RFLP analysis yielded the same differentiation among the strains, whereas the PCR amplification of delta sequences resulted the best method as it was fast and it showed a very high discriminative power. In any case, it has to be underlined that some strains, showing the same delta profiles, exhibited a different mtDNA restriction profile and electrophoretic karyotype, suggesting that more than one molecular marker is required for reliable strain discrimination. Although the techniques used revealed a different resolution power, they all revealed a genetic relationship among strains isolated from spontaneous fermentation of grapes of different origins. In fact, none of the typing methods was able to discriminate some strains isolated from different areas.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Saccharomyces cerevisiae Wine strains Minisatellites Karyotyping mtDNA-RFLP Interdelta region
Megjelenés:	Annals of Microbiology. - 63 : 2 (2013), p. 661-668. -
További szerzők:	Capece, Angela Sipiczki Mátyás (1948-) (biológus) Csoma Hajnalka (1979-) (biológus, szőlész-borász) Romano, Patrizia
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM088416
Első szerző:	Sipiczki Mátyás (biológus)
Cím:	Diversity, variability and fast adaptive evolution of the wine yeast (Saccharomyces cerevisiae) genome : a review / Matthias Sipiczki
Dátum:	2011
ISSN:	1590-4261 1869-2044
Megjegyzések:	Due to the high propensity for genomic alteration of their genomes, wine yeast (Saccharomyces cerevisiae) strains are very diverse. Genetic/genomic differences often correlate with different enological and technological properties. Experimental data indicate that the plasticity of the genome makes wine yeast populations capable of adapting to the continuously changing and rather harsh fermentation environment. A model is proposed for this fast adaptive genome evolution (FAGE) that explains the roles of the changing clonal composition of the population during fermentation, genome purification by meiosis at the end of fermentation and subsequent autodiploidisation of the spore clones in the next vintage, and the generation of new genomes through conjugation of non-sister spore clones (heterodiploidisation). Possibilities for genome stabilisation are also considered.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk genetic instability genome stabilisation segregation adaptation selection
Megjelenés:	Annals of Microbiology. - 61 : 1 (2011), p. 85-93. -
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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