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001-es BibID:	BIBFORM094639
Első szerző:	Horváth Enikő (biológus)
Cím:	Assaying the effect of yeasts on growth of fungi associated with disease / Horváth Enikő, Sipiczki Mátyás, Csoma Hajnalka, Miklós Ida
Dátum:	2020
ISSN:	1471-2180
Megjegyzések:	Abstract Background: Pathogenic fungi often cause serious infections mainly in immunocompromised persons. The number of infections caused by the non-albicans Candida or other species has significantly increased over the last years. These infections present a major challenge in the health sector because these pathogenic fungi have strong virulence and often show resistance to the commonly used antifungal treatments. To solve the problems caused by the drug resistant pathogenic fungi, it is necessary to find new antifungal agents and their sources. The aim of this study was to give evidence that yeasts can effectively fight against strains which belong to pathogenic fungi and reveal those yeasts which are able to inhibit growth of Kodamaea ohmeri, Pichia kudriavzevii, Naganishia albida or Candida tropicalis. Furthermore, we wanted to determine the effects of certain culturing factors on the growth inhibition. Results: Our screening revealed that although the strains belonging to pathogenic species were much more tolerant to the yeast-produced bioactive agents than the non-disease-associated yeasts, growth of Kodamaea ohmeri and Candida tropicalis could be inhibited by Metschnikowia andauensis, while Naganishia albida could be controlled by Pichia anomala or Candida tropicalis. Our data proved that the experimental circumstances could have a serious impact on the inhibitory capacity of the yeasts. Appearance of inhibition strongly depended on media, pH and temperature. Our data also shed some light on the fact that Pichia kudriavzevii must have high natural resistance to the yeast-produced agents, while other species, such as Saccharomycopsis crataegensis belonged to the easily inhibitable species. Conclusions: Our study suggests that yeast-produced bioactive agents could be potential growth inhibitory agents against the disease-associated fungi and yeasts can also contribute to alternative approaches to combat against pathogenic fungi. Our data revealed an important role of the culturing factors in inhibition and pointed to the complex nature of antagonism.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Disease-associated fungi Bioactive agents Yeast Antagonism Metschnikowia sp. Non-albicans Candida species Natural resistance
Megjelenés:	Bmc Microbiology. - 20 : 320 (2020), p. 1-10. -
További szerzők:	Sipiczki Mátyás (1948-) (biológus) Csoma Hajnalka (1979-) (biológus, szőlész-borász) Miklós Ida (1962-) (biológus)
Pályázati támogatás:	EFOP-3.6.1-16-2016-00022 EFOP FIK-20428-3/2018 Egyéb
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001-es BibID:	BIBFORM094560
035-os BibID:	(Cikkazonosító)10593 (WOS)000658822200030 (Scopus)85106032076
Első szerző:	Horváth Enikő (biológus)
Cím:	The antagonistic Metschnikowia andauensis produces extracellular enzymes and pulcherrimin, whose production can be promoted by the culture factors / Enikő Horváth, Lívia Dályai, Erna Szabó, Teréz Barna, László Kalmár, József Posta, Matthias Sipiczki, Hajnalka Csoma, Ida Miklós
Dátum:	2021
ISSN:	2045-2322
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Scientific Reports. - 11 (2021), p. 10593-10603. -
További szerzők:	Dályai Lívia Szabó Erna (1990-) (Biológus) Barna Teréz (1963-) (vegyész) Kalmár László Posta József (1948-) (vegyész, analitikus) Sipiczki Mátyás (1948-) (biológus) Csoma Hajnalka (1979-) (biológus, szőlész-borász) Miklós Ida (1962-) (biológus)
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001-es BibID:	BIBFORM055087
Első szerző:	Pfliegler Valter Péter (molekuláris biológus)
Cím:	Diversity of Candida zemplinina isolates inferred from RAPD, micro/minisatellite and physiological analysis / Walter P. Pfliegler, Enikő Horváth, Zoltán Kállai, Matthias Sipiczki
Dátum:	2014
ISSN:	0944-5013
Megjegyzések:	Among non-Saccharomyces wine yeasts, Candida zemlpinina is one of the frequently isolated and oenologically important species. It is mostly known from European winemaking areas and it has become one of the key species of non-Saccharomyces wine yeasts to study. Investigating the diversity of C. zemplinina isolates is important for a deeper understanding of the non-Saccharomyces wine yeasts and for the yeast starter industry, as numerous researches have pointed to the potential use of this species in winemaking. For assessing the biodiversity of a larger number of strains, RAPD and micro/minisatellite PCR is often the method of choice, however, this technique is often unstandardized. Whereas some laboratories use these methods for species identifications, others apply RAPD primers for determining intraspecies diversity. In this study, we have tested 5 different RAPD and micro/minisatellite primers on strains of C. zemplinina isolated from different locations. We show that after a rigorous PCR-optimization aimed at reproducibility and comparability of band patterns with these PCR-reactions, diversity of different strains from a wide range of geographic locations is relatively low. The analysis of several oenologically important physiological traits of the strains showed a relatively low level of diversity as well. We also demonstrate that the intraspecific diversity of C. zemplinina observable with different techniques (RAPD, micro/minisatellite or physiological analysis) may be fairly different and not necessarily comparable.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Microbiological Research. - 169 : 5-6 (2014), p. 402-410. -
További szerzők:	Horváth Enikő (1987-) (biológus) Kállai Zoltán (1986-) (biológus) Sipiczki Mátyás (1948-) (biológus)
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001-es BibID:	BIBFORM074051
035-os BibID:	(cikkazonosító)1193 (WOS)000434951800001 (Scopus)85048341000
Első szerző:	Sipiczki Mátyás (biológus)
Cím:	Birth-and-Death Evolution and Reticulation of ITS Segments of Metschnikowia andauensis and Metschnikowia fructicola rDNA Repeats / Sipiczki Matthias, Horvath Eniko, Pfliegler Walter P.
Dátum:	2018
ISSN:	1664-302X
Megjegyzések:	The internal transcribed spacer (ITS) region (ITS1, 5.8S rDNA, and ITS2) separates the genes coding for the SSU 18S and the LSU 26S genes in the rDNA units which are organized into long tandem arrays in the overwhelming majority of fungi. As members of a multigenic family, these units are subject of concerted evolution, which homogenizes their sequences. Exceptions have been observed in certain groups of plants and in a few fungal species. In our previous study we described exceptionally high degree of sequence diversity in the D1/D2 domains of two pulcherrimin-producing Metschnikowia (Saccharomycotina) species which appeared to evolve by reticulation. The major goals of this study were the examination of the diversity of the ITS segments and their evolution. We show that the ITS sequences of these species are not homogenized either, differ from each other by up to 38 substitutions and indels which have dramatic effects on the predicted secondary structures of the transcripts. The high intragenomic diversity makes the D1/D2 domains and the ITS spacers unsuitable for barcoding of these species and therefore the taxonomic position of strains previously assigned to them needs revision. By analyzing the genome sequence of the M. fructicola type strain, we also show that the rDNA of this species is fragmented, contains pseudogenes and thus evolves by the birth-and-death mechanism rather than by homogenisation, which is unusual in yeasts. The results of the network analysis of the sequences further indicate that the ITS regions are also involved in reticulation. M. andauensis and M. fructicola can form interspecies hybrids and their hybrids segregate, providing thus possibilities for reticulation of the rDNA repeats.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk structure prediction yeast ribosomal RNA ITS evolution pseudogene reticulation hybridisation
Megjelenés:	Frontiers in Microbiology. - 9 (2018), p. 1-16. -
További szerzők:	Horváth Enikő (1987-) (biológus) Pfliegler Valter Péter (1986-) (molekuláris biológus)
Pályázati támogatás:	K-124417 Egyéb TÁMOP-4.2.4.A/2-11/1-2012-0001 Egyéb
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