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001-es BibID:	BIBFORM108232
035-os BibID:	(cikkazonosító)1112 (WoS)000985396000014 (Scopus)85146613535
Első szerző:	Antunovics Zsuzsa (molekuláris biológus, genetikus)
Cím:	Synthetic two-species allodiploid and three-species allotetraploid Saccharomyces hybrids with euploid (complete) parental subgenomes / Zsuzsa Antunovics, Adrienn Szabo, Lina Heistinger, Diethard Mattanovich, Matthias Sipiczki
Dátum:	2023
ISSN:	2045-2322
Megjegyzések:	Combination of the genomes of Saccharomyces species has great potential for the construction of new industrial strains as well as for the study of the process of speciation. However, these species are reproductively isolated by a double sterility barrier. The first barrier is mainly due to the failure of the chromosomes to pair in allodiploid meiosis. The second barrier ensures that the hybrid remains sterile even after genome duplication, an event that can restore fertility in plant interspecies hybrids. The latter is attributable to the autodiploidisation of the allotetraploid meiosis that results in sterile allodiploid spores (return to the first barrier). Occasionally, mating-competent alloaneuploid spores arise by malsegregation of MAT-carrying chromosomes. These can mate with cells of a third species resulting in aneuploid zygotes having at least one incomplete subgenome. Here we report on the construction of euploid three-species hybrids by making use of "rare mating" between a sterile S. kudriavzevii x S. uvarum allodiploid hybrid and a diploid S. cerevisiae strain. The hybrids have allotetraploid 2nScnSk nSu genomes consisting of complete sets of parental chromosomes. This is the first report on the production of euploid three-species Saccharomyces hybrids by natural mating, without genetic manipulation. The hybrids provide possibilities for studying the interactions of three allospecific genomes and their orthologous genes present in the same cell.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Scientific Reports. - 13 : 1 (2023), p. 1-13. -
További szerzők:	Szabó Adrienn (1992-) (biológus) Heistinger, Lina Mattanovich, Diethard Sipiczki Mátyás (1948-) (biológus)
Pályázati támogatás:	2020-1.1.2.-PIACI-KFI-2020-00130 Egyéb K-124417 NKFIH
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM081363
035-os BibID:	(cikkazonosító)fow037
Első szerző:	Bellasio, Martina
Cím:	Complete genome sequence and transcriptome regulation of the pentose utilizing yeast Sugiyamaella lignohabitans / Martina Bellasio, Armin Peymann, Matthias G. Steiger, Minoska Valli, Matthias Sipiczki, Michael Sauer, Alexandra B. Graf, Hans Marx, Diethard Mattanovich
Dátum:	2016
ISSN:	1567-1356 1567-1364
Megjegyzések:	Efficient conversion of hexoses and pentoses into value-added chemicals represents one core step for establishing economically feasible biorefineries from lignocellulosic material. While extensive research efforts have recently provided advances in the overall process performance, the quest for new microbial cell factories and novel enzymes sources is still open. As demonstrated recently the yeast Sugiyamaella lignohabitans (formerly Candida lignohabitans) represents a promising microbial cell factory for the production of organic acids from lignocellulosic hydrolysates. We report here the de novo genome assembly of S. lignohabitans using the Single Molecule Real-Time platform, with gene prediction refined by using RNA-seq. The sequencing revealed a 15.98 Mb genome, subdivided into four chromosomes. By phylogenetic analysis, Blastobotrys (Arxula) adeninivorans and Yarrowia lipolytica were found to be close relatives of S. lignohabitans. Differential gene expression was evaluated in typical growth conditions on glucose and xylose and allowed a first insight into the transcriptional response of S. lignohabitans to different carbon sources and different oxygenation conditions. Novel sequences for enzymes and transporters involved in the central carbon metabolism, and therefore of potential biotechnological interest, were identified. These data open the way for a better understanding of the metabolism of S. lignohabitans and provide resources for further metabolic engineering.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Sugiyamaella lignohabitans complete genome sequence RNA-Seq SMRT sequencing microbial cell factory
Megjelenés:	FEMS Yeast Research. - 16 : 4 (2016), p. 1-14. -
További szerzők:	Peymann, Armin Steiger, Matthias G. Valli, Minoska Sipiczki Mátyás (1948-) (biológus) Sauer, Michael Graf, Alexandra B. Marx, Hans Mattanovich, Diethard
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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