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001-es BibID:	BIBFORM067884
Első szerző:	Bácskai Tímea (biológus, neurobiológus)
Cím:	Effect of fluorokinolone treatment on the peptidergic innervation of the salivary glands / Tímea Bácskai, Barna Kelentey, Ádám Deák, Tivadar Zelles, Boglárka Skopkó, Klara Matesz
Dátum:	2010
Megjegyzések:	Fluorokinolones (i.e. Peflacine, PEF) are used in the dental and medical therapy. It was demonstrated that chronic treatment on the rats resulted in disturbance in the secretory function of the salivary glands accompanied by the morphological sign of atrophy in the secretory units. The mechanism of this impairment is unknown. Because the peripheral neuropathy was previously described as the toxic side effect of the chronic PEF treatment we proposed that the morphological and functional disorder of salivary glands developed on the basis of a neuronal disorder. Earlier studies described, that the mast cells could release nerve growth factor (NGF), which is important in the surviving of neurons. The lack of NGF results degenerative processes in the peripheral neurons which can change the expression of neuropeptides (i.e. serotonine, SER). The aim of this study was to determine the number of mast cells and the qualitative and quantitative changes of SER immunoreactive (IR) nerve terminals in the salivary glands after PEF treatment. Adult rats were treated with PEF for 3 and 7 days. For the visualization of mast cell we have used Toluidine blue staining. Immunohistochemical methods were used to detect the SER containing fibers on the salivary glands. After the chronic treatment we could detect the increased number of mast cells which supports the protective role of the NGF. The number of SER IR fibers decreased compared to the control. The changes in the number of IR fibers support our previous results, that local denervation of salivary gland can cause the atrophy of the acini. taa (hibás)
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idézhető absztrakt saliva
Megjelenés:	Frontiers in Neuroscience 2010 (2010), p. 1. -
További szerzők:	Kelentey Barna (1959-) (fogszakorvos) Deák Ádám (1974-) (állatorvos) Zelles Tivadar Skopkó Boglárka Emese (1986-) (fogszakorvos) Matesz Klára (1949-) (anatómus, neurobiológus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM109585
035-os BibID:	(cikkazonosító)1115685 (Scopus)85150718609 (WoS)000951373400001
Első szerző:	Ducza László (molekuláris biológus)
Cím:	Neuronal P2X4 receptor may contribute to peripheral inflammatory pain in rat spinal dorsal horn / Ducza László, Gajtkó Andrea, Hegedűs Krisztina, Bakk Erzsébet, Kis Gréta, Gaál Botond, Takács Roland, Szücs Péter, Matesz Klára, Holló Krisztina
Dátum:	2023
ISSN:	1662-5099
Megjegyzések:	Objective: Intense inflammation may result in pain, which manifests as spinal central sensitization. There is growing evidence that purinergic signaling plays a pivotal role in the orchestration of pain processing. Over the last decade the ionotropic P2X purino receptor 4 (P2X4) got into spotlight in neuropathic disorders, however its precise spinal expression was scantily characterized during inflammatory pain. Thus, we intended to analyze the receptor distribution within spinal dorsal horn and lumbar dorsal root ganglia (DRG) of rats suffering in inflammatory pain induced by complete Freund adjuvant (CFA). Methods: CFA-induced peripheral inflammation was validated by mechanical and thermal behavioral tests. In order to ensure about the putative alteration of spinal P2X4 receptor gene expression qPCR reactions were designed, followed by immunoperoxidase and Western blot experiments to assess changes at a protein level. Colocalization of P2X4 with neuronal and glial markers was investigated by double immunofluorescent labelings, which were subsequently analyzed with IMARIS software. Transmission electronmicroscopy was applied to study the ultrastructural localization of the receptor. Concurrently, in lumbar DRG cells similar methodology has been carried out to complete our observations. Results: The figures of mechanical and thermal behavioral tests proved the establishment of CFA-induced inflammatory pain. We observed significant enhancement of P2X4 transcript level within the spinal dorsal horn 3?days upon CFA administration. Elevation of P2X4 immunoreactivity within Rexed lamina I-II of the spinal gray matter was synchronous with mRNA expression, and confirmed by protein blotting. According to IMARIS analysis the robust protein increase was mainly detected on primary afferent axonterminals and GFAP-labelled astrocyte membrane compartments, but not on postsynaptic dendrites was also validated ultrastructurally within the spinal dorsal horn. Furthermore, lumbar DRG analysis demonstrated that peptidergic and non-peptidergic nociceptive subsets of ganglia cells were also abundantly positive for P2X4 receptor in CFA model. Conclusion: Here we provide novel evidence about involvement of neuronal and glial P2X4 receptor in the establishment of inflammatory pain.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk inflammatory pain spinal dorsal horn P2X4 receptor central sensitization primary afferents glial cells dorsal root ganglia
Megjelenés:	Frontiers in Molecular Neuroscience. - 16 (2023), p. 1-16. -
További szerzők:	Gajtkó Andrea (1989-) (molekuláris biológus) Hegedűs Krisztina Bakk Erzsébet Kis Gréta (1979-) (molekuláris biológus) Gaál Botond Ágoston (1982-) (anatómus, neurobiológus) Takács Roland Ádám (1985-) (molekuláris biológus, biokémikus) Szűcs Péter (1974-) (kutatóorvos) Matesz Klára (1949-) (anatómus, neurobiológus) Holló Krisztina (1967-) (vegyész)
Pályázati támogatás:	KTIA_NAP_13-2-2014-0005 Egyéb 2017-1.2.1-NKP-2017-00002 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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