Találati lista | Tudóstér

001-es BibID:	BIBFORM029726
Első szerző:	Kulik Ákos
Cím:	Sub-population of capsaicin sensitive primary afferent neurons in thoracic, lumbar and sacral dorsal root ganglion in young rats revealed by stimulated cobalt uptake / Kulik A., Polgár E., Matesz K., Kothalawala S., Szűcs P., Nagy I.
Dátum:	1996
ISSN:	0236-5383
Megjegyzések:	The number of dorsal root ganglion (DRG) neurons, the relative number of capsaicin sensitive DRG cells and the diameter distribution of these neurons were investigated in the thoracic 11, lumbar 5 and sacral 1 DRG ganglia in young rats. The capsaicin sensitivity of DRG cells was shown by the stimulated cobalt uptake technique in in vitro conditions. Cobalt labelled and non-labelled neurons were counted using the dissector method. Our results show that the total number of DRG cells in the Th11, L5 and S1 segments (4200-6500 per ganglion) were not significantly different from each other and about 8% of these cells were capsaicin sensitive in the segments studied. These findings also show that the capsaicin sensitive DRG cells belong to the small diameter DRG cell population. Control experiments on neonatally capsaicin injected animals indicate that the capsaicin stimulated cobalt uptake provides selective and specific staining of capsaicin sensitive DRG neurons.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény hazai lapban
Megjelenés:	Acta Biologica Hungarica. - 47 : 1-4 (1996), p. 251-259. -
További szerzők:	Polgár E. Matesz Klára (1949-) (anatómus, neurobiológus) Kothalawala, D. S. Szűcs Péter (1974-) (kutatóorvos) Nagy I. (orvos)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM029519
Első szerző:	Nagy I. (orvos)
Cím:	Cobalt uptake enables identification of capsaicin and bradykinin sensitive sub populations of rat dorsal root ganglion cells in vitro / Nagy I., Pabla R., Matesz K., Dray A., Woolf C., Urban L.
Dátum:	1993
ISSN:	0306-4522
Megjegyzések:	A novel modification of the stimulated cobalt uptake technique has been used to identify rat dorsal root ganglion cells expressing capsaicin and bradykinin receptors. The technique involves incubating intact dorsal root ganglia in vitro in a modified Krebs solution in which cobalt chloride has been substituted for calcium. Activation of dorsal root ganglion cells by capsaicin or bradykinin in the presence of the cobalt ions results in cobalt influx into the excited cells. Histochemical methods were then used to visualize the intracellular accumulation of cobalt, and labelled cells were counted and characterized. Capsaicin (2 microM) or bradykinin (500 nM) applied for 20 min induced cobalt uptake in 13.8 +/- 0.6 and 9.6 +/- 0.5% of neuronal profiles in dorsal root ganglia (L4), respectively, a significantly larger number than stained in control ganglia (in the absence of agonists: 1.8 +/- 0.7%). The longest diameter of the soma of stained dorsal root ganglion cells following capsaicin and bradykinin perfusion were significantly different from each other and from the non-labelled population (17.5 +/- 0.7 and 24.5 +/- 0.2 microns for capsaicin; 23.2 +/- 0.9 and 25.5 +/- 0.4 microns for bradykinin; labelled and non-labelled cells, respectively). The distribution of cell diameters revealed that while capsaicin-sensitive cells were exclusively small-sized, bradykinin-sensitive cells were predominantly small and medium sized. The selective bradykinin-2 receptor antagonist HOE-140 (5.0 microM) blocked the bradykinin-induced staining (2.16 +/- 0.02%) but not that of capsaicin. The bradykinin-1 agonist [des-Arg9]-bradykinin did not induce any significant increase in stained cells over the control number (2.2 +/- 0.7%)
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Neuroscience. - 56 : 1 (1993), p. 241-246. -
További szerzők:	Pabla, R. Matesz Klára (1949-) (anatómus, neurobiológus) Dray, A. Woolf, C. Urbán László (London, UK)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
Borító:
Saját polcon: