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001-es BibID:BIBFORM027871
Első szerző:Csordás Tóth Éva
Cím:Protein phosphatase 2A holoenzyme and its subunits from Medicago sativa / Éva Csordás Tóth, Emese Vissi, Izabella Kovács, Attila Szőke, Joaquín Ariño, Pál Gergely, Dénes Dudits, Viktor Dombrádi
Dátum:2000
ISSN:0167-4412
Megjegyzések:We detected an about 200 kDa holoenzyme of protein phosphatase 2A (PP2A) in the crude extract of Medicago sativa microcallus cells by gel permeation chromatography. By polymerase chain reaction (PCR) we isolated two M. sativa cDNA fragments corresponding to the catalytic (C) subunit, and one each coding for the A and the B regulatory subunits of PP2A. The C subunit sequences were different from that published previously, indicating the existence of at least three different isoforms in M. sativa. Using the PCR fragments as probes, we obtained two distinct full-length clones for both the A and B subunits from an alfalfa cDNA library. Our results demonstrate that the components of the PP2A holoenzyme, namely the catalytic and regulatory subunits, are present in alfalfa in several isoforms and that their sequences are highly similar to their plant, yeast and animal counterparts. The distinct regulatory subunit genes are constitutively expressed during the cell cycle. Interestingly, two A-B subunit pairs had parallel mRNA steady-state levels in different plant tissues suggesting that not all of the possible isoform combinations are present in all tissues. The expression of the MsPP2A Bbeta subunit form was induced by abscisic acid indicating a specific function for this protein in the stress response.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
cDNA cloning
quaternary structure
Ser/Thr protein phosphatase
stress response
tissue-specific expression
Megjelenés:Plant Molecular Biology. - 43 : 4 (2000), p. 527-536. -
További szerzők:Kovács Izabella (Szeged) Szőke Attila Ariño, Joaquín Dudits Dénes Vissi Emese (1968-) (biokémikus, biológus) Gergely Pál (1947-) (biokémikus) Dombrádi Viktor (1953-) (biokémikus)
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2.

001-es BibID:BIBFORM027869
Első szerző:Vissi Emese (biokémikus, biológus)
Cím:The protein phosphatases and their functions in plants / Emese Vissi, Éva Csordás-Tóth, Ferhan Ayaydin, Endre Kókai, Pál Gergely, Dénes Dudits, Viktor Dombrádi
Dátum:2001
ISBN:1586031805
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
protein phosphatases
plants
Megjelenés:Protein Modulates in Cellular Signaling / Heilmeyer L; Friedrich P;. - p. 195-201. -
További szerzők:Csordás Tóth Éva Ayaydin, Ferhan Dudits Dénes Kókai Endre (1971-) (biokémikus, biológus) Gergely Pál (1947-) (biokémikus) Dombrádi Viktor (1953-) (biokémikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM027917
Első szerző:Vissi Emese (biokémikus, biológus)
Cím:Protein phosphatase 1 catalytic subunit isoforms from alfalfa : biochemical characterization and cDNA cloning / Emese Vissi, Éva Csordás Tóth, Izabella Kovács, Zoltán Magyar, Gábor V. Horváth, Péter Bagossi, Pál Gergely, Dénes Dudits, Viktor Dombrádi
Dátum:1998
Megjegyzések:The catalytic subunit of protein phosphatase 1 (PP1c) was purified from an alfalfa (Medicago sativa) microcallus cell culture. The preparation was inhibited by rabbit muscle inhibitor-2 and okadaic acid and had a molecular mass of 35 kDa. Five distinct cDNAs termed MsPP1alpha, -beta, -gamma, -delta, and -epsilon were cloned from a M. sativa somatic embryo library. MsPP1alpha was identical to a cDNA reported earlier [A. Páy, M. Pirck, L. Bögre, H. Hirt, and E. Heberle-Bors Mol. Gen. Genet. 244, 176-182, 1994], while the others represented novel isoforms encoded by separate genes. The predicted amino acid sequences of MsPP1alpha, -beta, -gamma, -delta, and -epsilon were highly similar to each other and to other known PP1c sequences. The GST-MsPP1ss fusion protein expressed in Escherichia coli was catalytically active and was inhibited by inhibitor-2 and okadaic acid. Affinity-purified polyclonal MsPP1antipeptide antibody detected a protein of 36 kDa in crude cell extracts. These results proved that the cDNA clone encoded an active PP1c which was very similar to the purified enzyme. The mRNA and protein concentrations of PP1c as well as the specific activity of protein phosphatase 1 did not change during the cell cycle in a synchronized alfalfa cell culture. On the other hand, the isoforms exhibited different steady-state mRNA levels in different plant organs suggesting tissue-specific functions.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
protein phosphatase1
Medicago sativa
protein purification
cDNA sequencing
cell cycle
gene expression
egyetemen (Magyarországon) készült közlemény
Megjelenés:Archives of Biochemistry and Biophysics. - 360 : 2 (1998), p. 206-214. -
További szerzők:Csordás Tóth Éva Kovács Izabella (Szeged) Magyar Zoltán Horváth Gábor V. Bagossi Péter (1966-2011) (biokémikus, vegyész) Dudits Dénes Gergely Pál (1947-) (biokémikus) Dombrádi Viktor (1953-) (biokémikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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