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001-es BibID:	BIBFORM027871
Első szerző:	Csordás Tóth Éva
Cím:	Protein phosphatase 2A holoenzyme and its subunits from Medicago sativa / Éva Csordás Tóth, Emese Vissi, Izabella Kovács, Attila Szőke, Joaquín Ariño, Pál Gergely, Dénes Dudits, Viktor Dombrádi
Dátum:	2000
ISSN:	0167-4412
Megjegyzések:	We detected an about 200 kDa holoenzyme of protein phosphatase 2A (PP2A) in the crude extract of Medicago sativa microcallus cells by gel permeation chromatography. By polymerase chain reaction (PCR) we isolated two M. sativa cDNA fragments corresponding to the catalytic (C) subunit, and one each coding for the A and the B regulatory subunits of PP2A. The C subunit sequences were different from that published previously, indicating the existence of at least three different isoforms in M. sativa. Using the PCR fragments as probes, we obtained two distinct full-length clones for both the A and B subunits from an alfalfa cDNA library. Our results demonstrate that the components of the PP2A holoenzyme, namely the catalytic and regulatory subunits, are present in alfalfa in several isoforms and that their sequences are highly similar to their plant, yeast and animal counterparts. The distinct regulatory subunit genes are constitutively expressed during the cell cycle. Interestingly, two A-B subunit pairs had parallel mRNA steady-state levels in different plant tissues suggesting that not all of the possible isoform combinations are present in all tissues. The expression of the MsPP2A Bbeta subunit form was induced by abscisic acid indicating a specific function for this protein in the stress response.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban cDNA cloning quaternary structure Ser/Thr protein phosphatase stress response tissue-specific expression
Megjelenés:	Plant Molecular Biology. - 43 : 4 (2000), p. 527-536. -
További szerzők:	Kovács Izabella (Szeged) Szőke Attila Ariño, Joaquín Dudits Dénes Vissi Emese (1968-) (biokémikus, biológus) Gergely Pál (1947-) (biokémikus) Dombrádi Viktor (1953-) (biokémikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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001-es BibID:	BIBFORM027865
Első szerző:	Vissi Emese (biokémikus, biológus)
Cím:	Functional analysis of the Neurospora crassa PZL-1 protein phosphatase by expression in budding and fission yeast / Emese Vissi, Josef Clotet, Eulalia de Nadal, Anna Barceló, Éva Bakó, Pál Gergely, Viktor Dombrádi, Joaquín Ariño
Dátum:	2001
ISSN:	0749-503X
Megjegyzések:	The gene pzl-1 from the filamentous fungus Neurospora crassa encodes a putative Ser/Thr protein phosphatase that is reminiscent of the Ppz1/Ppz2 and Pzh1 phosphatases from Saccharomyces cerevisiae and Schizosaccharomyces pombe, respectively. The entire PZL-1 protein, as well as its carboxyl-terminal domain, have been expressed in Escherichia coli as active protein phosphatases. To characterize its cellular role, PZL-1 was also expressed in Sz. pombe and in S. cerevisiae. Expression of PZL-1 in S. cerevisiae from the PPZ1 promoter was able to rescue the altered sensitivity to caffeine and lithium ions of a ppz1 strain. Furthermore, high copy number expression of PZL-1 alleviated the lytic phenotype of a S. cerevisiae slt2/mpk1 mitogen-activated protein (MAP) kinase mutant, similarly to that described for PPZ1, and mimicked the effects of high levels of Ppz1 on cell growth. Expression of PZL-1 in fission yeast from a weak version of the nmt1 promoter fully rescued the growth defect of a pzh1Delta strain in high potassium, but only partially complemented the sodium-hypertolerant phenotype. Strong overexpression of the N. crassa phosphatase in Sz. pombe affected cell growth and morphology. Therefore, PZL-1 appears to fulfil every known function carried out by its S. cerevisiae counterpart, despite the marked divergence in sequence within their NH(2)-terminal moieties.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban PPZ phosphatases functional expression yeast salt tolerance külföldön készült közlemény
Megjelenés:	Yeast. - 18 (2001), p. 115-124. -
További szerzők:	Clotet, Josef de Nadal, Eulalia Barceló, Anna Ariño, Joaquín Bakó Éva (1958-) (biokémikus) Gergely Pál (1947-) (biokémikus) Dombrádi Viktor (1953-) (biokémikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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