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001-es BibID:BIBFORM068277
Első szerző:Csongrádi Éva (szakorvos)
Cím:Adipokines as atherothrombotic risk factors in obese subjects : associations with haemostatic markers and common carotid wall thickness / É. Csongrádi, M. Káplár, B. Nagy Jr., C. A. Koch, A. Juhász, L. Bajnok, Zs. Varga, I. Seres, Zs. Karányi, M. T. Magyar, L. Oláh, A. Facskó, J. Kappelmayer, Gy. Paragh
Dátum:2017
ISSN:0939-4753
Megjegyzések:Background and aims: Some crucial associations between obesity-related altered adipokinelevels and the main factors of atherosclerotic, atherothrombotic processes are not fully known.We analysed the relationships of classic adipokines, namely leptin, resistin, adiponectin, tumour necrosisfactor-alpha (TNF-a), interleukin 6 (IL-6) with the markers of platelet activation, including meanplatelet volume (MPV), platelet surface/soluble P-selectin, platelet-derived microparticles (PMPs), theparameters of coagulation abnormalities and common carotid intima-media thickness (IMT) in obesepatients with or without atheroscleroticcomorbidities in comparison to age- and sex-matched controls.Methods and results: We enrolled 154 obese individuals, including 98 suffering from atheroscleroticconcomitant conditions, 56 free of atherosclerotic comorbidities and 62 healthy controls. Plasma levelsof leptin, resistin, adiponectin, TNF-a, IL-6, soluble P-selectin, and plasminogen activator inhibitor-1 antigen(PAI-1 ag) were analysed by ELISA. Platelet surface P-selectin and PMPs were measured by flowcytometry. IMT was detected by ultrasonography. Adipokines were closely associated withmarkers ofplatelet hyperactivity, hypercoagulability, hypofibrinolysis and IMT. Significant independent associationswere found between leptin and platelet count (p < 0.0001), MPV (p Z 0.019), PMPs(p < 0.0001), fibrinogen (pZ0.001), factor VIII (FVIII) activity (pZ0.035); adiponectin and PAI-1 ag(pZ0.035); resistin and soluble P-selectin (pZ0.002); TNF-a and PAI-1 ag (p < 0.0001); and IL-6and fibrinogen (pZ 0.011). Finally, leptin (p Z 0.0005), adiponectin (pZ 0.019), IL-6 (p Z 0.001),MPV(pZ0.0003), PMP (pZ0.008), and FVIII activity (pZ0.043)were independent predictors of IMT.Conclusion: Overall,we suggest that inobese subjects altered adipokinelevels playa key role incommoncarotid atherosclerosis both directly and through haemostatic parameters.
Tárgyszavak:Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban
Obesity
Adipokines
Atherosclerosis
Haemostasis
Common carotid intima-media thickness
Megjelenés:Nutrition Metabolism And Cardiovascular Diseases 27 : 6 (2017), p. 571-580. -
További szerzők:Káplár Miklós (1965-) (belgyógyász, diabetológus) Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos) Koch, Christian A. Juhász Attila (1970-) (szakorvos, klinikai mikrobiológus) Bajnok László (1961-) (belgyógyász) Varga Zsuzsa (1951-) (biokémikus, nephrológus) Seres Ildikó (1954-) (biokémikus) Karányi Zsolt (1961-) (biostatisztikus, bioinformatikus) Magyar Mária Tünde (1970-) (neurológus) Oláh László (1967-) (neurológus) Facskó Andrea (1953-) (szemész) Kappelmayer János (1960-) (laboratóriumi szakorvos) Paragh György (1953-) (belgyógyász)
Pályázati támogatás:K75199
OTKA
TÁMOP 4.2.1/B-091/1/KONV-2010-0007
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2.

001-es BibID:BIBFORM061623
Első szerző:Csongrádi Éva (szakorvos)
Cím:A Thr715Pro P-selectin gén polimorfizmus hatása a thrombocyta aktivációra obesitasban / Csongrádi Éva, Káplár Miklós, Nagy Béla, Varga Zsuzsa, Seres Ildikó, Juhász Attila, Bajnok László, Karányi Zsolt, Kappelmayer János, Paragh György;
Dátum:2008
Tárgyszavak:Orvostudományok Klinikai orvostudományok konferenciacikk
Megjelenés:Obesitologia Hungarica. - 9 : Suppl. 1 (2008), p. S52. -
További szerzők:Káplár Miklós (1965-) (belgyógyász, diabetológus) Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos) Varga Zsuzsa (1951-) (biokémikus, nephrológus) Seres Ildikó (1954-) (biokémikus) Juhász Attila (1970-) (szakorvos, klinikai mikrobiológus) Bajnok László (1961-) (belgyógyász) Karányi Zsolt (1961-) (biostatisztikus, bioinformatikus) Kappelmayer János (1960-) (laboratóriumi szakorvos) Paragh György (1953-) (belgyógyász)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM023862
Első szerző:Csongrádi Éva (szakorvos)
Cím:Increased levels of platelet activation markers are positively associated with carotid wall thickness and other atherosclerotic risk factors in obese patients / Csongrádi Éva, Nagy Béla Jr., Fülöp Tamás, Varga Zsuzsa, Karányi Zsolt, Magyar Mária T., Oláh László, Papp Mária, Facskó Andrea, Kappelmayer János, Paragh György, Káplár Miklós
Dátum:2011
ISSN:0340-6245
Megjegyzések:The role of platelets in the development of atherosclerosis and obesity-related prothrombotic state is still under investigation. In this cross-sectional cohort study, we measured the levels of different platelet activation markers and evaluated their relationship with carotid intima-media thickness (IMT) along with other atherosclerotic risk factors in obese patients with or without atherosclerotic co-morbidities. We enrolled 154 obese patients, including 98 with either hypertension, type 2 diabetes mellitus or dyslipidaemia, 56 without these co-morbidities and 62 age- and sex-matched healthy controls. Platelet P-selectin expression and the number of platelet-derived microparticles (PMPs) were measured by flow cytometry; soluble P-selectin levels were analysed by ELISA and Thr715Pro P-selectin polymorphism was determined by PCR-RFLP. Carotid IMT was examined by ultrasonography. The levels of platelet activation parameters were significantly elevated in all obese subjects with increased carotid IMT compared to healthy controls. There was no effect of Thr715Pro genotype on soluble P-selectin levels in obese individuals contrary to normal subjects. Significant and positive association was revealed between carotid IMT and platelet P-selectin (p<0.0001), soluble P-selectin (p=0.039) and PMP (p=0.0001) levels. After adjusting for multiple variables, independent association was found between soluble P-selectin and fibrinogen (p=0.007), PMP levels and body mass index (p<0.0001) as well as platelet P-selectin and carotid IMT (p=0.012) plus plasminogen activator inhibitor-1 (p=0.009). In conclusion, P-selectin and PMP levels showed positive associations with abnormal carotid IMT and other risk factors in obesity suggesting a critical role of enhanced platelet reactivity in atherosclerotic wall alteration.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Thrombosis And Haemostasis 106 : 4 (2011), p. 683-692. -
További szerzők:Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos) Fülöp Tamás (1928-) (népegészségügyi szakember, egészségfejlesztő) Varga Zsuzsa (1951-) (biokémikus, nephrológus) Karányi Zsolt (1961-) (biostatisztikus, bioinformatikus) Magyar Mária Tünde (1970-) (neurológus) Oláh László (1967-) (neurológus) Papp Mária (1975-) (belgyógyász, gasztroenterológus) Facskó Andrea (1953-) (szemész) Kappelmayer János (1960-) (laboratóriumi szakorvos) Paragh György (1953-) (belgyógyász) Káplár Miklós (1965-) (belgyógyász, diabetológus)
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4.

001-es BibID:BIBFORM085388
035-os BibID:(WoS)000524776500001 (Scopus)85082380165 (cikkazonosító)228
Első szerző:Erdei Judit Zsuzsa (vegyész)
Cím:The Role of Hemoglobin Oxidation Products in Triggering Inflammatory Response Upon Intraventricular Hemorrhage in Premature Infants / Erdei Judit, Tóth Andrea, Nagy Andrea, Nyakundi Benard Bogonko, Fejes Zsolt, Nagy Béla Jr., Novák László, Bognár László, Balogh Enikö, Paragh György, Kappelmayer János, Bácsi Attila, Jeney Viktória
Dátum:2020
ISSN:1664-3224
Megjegyzések:Intraventricular hemorrhage (IVH) is a frequent complication of prematurity that is associated with high neonatal mortality and morbidity. IVH is accompanied by red blood cell (RBC) lysis, hemoglobin (Hb) oxidation, and sterile inflammation. Here we investigated whether extracellular Hb, metHb, ferrylHb, and heme contribute to the inflammatory response after IVH. We collected cerebrospinal fluid (CSF) (n = 20) from premature infants with grade III IVH at different time points after the onset of IVH. Levels of Hb, metHb, total heme, and free heme were the highest in CSF samples obtained between days 0 and 20 after the onset of IVH and were mostly non-detectable in CSF collected between days 41 and 60 of post-IVH. Besides Hb monomers, we detected cross-linked Hb dimers and tetramers in post-IVH CSF samples obtained in days 0-20 and 21-40, but only Hb tetramers were present in CSF samples obtained after 41-60 days. Vascular cell adhesion molecule-1 (VCAM-1) and interleukin-8 (IL-8) levels were higher in CSF samples obtained between days 0 and 20 than in CSF collected between days 41 and 60 of post-IVH. Concentrations of VCAM-1, intercellular adhesion molecule-1 (ICAM-1), and IL-8 strongly correlated with total heme levels in CSF. Applying the identified heme sources on human brain microvascular endothelial cells revealed that Hb oxidation products and free heme contribute to the inflammatory response. We concluded that RBC lysis, Hb oxidation, and heme release are important components of the inflammatory response in IVH. Pharmacological interventions targeting cell-free Hb, Hb oxidation products, and free heme could have potential to limit the neuroinflammatory response following IVH.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Frontiers in Immunology. - 11 (2020), p. 228. -
További szerzők:Tóth Andrea (1992-) (molekuláris biológus) Nagy Andrea (1958-) (csecsemő és gyermekgyógyász, neonatológus) Nyakundi, Benard Bogonko (1983-) (biokémikus) Fejes Zsolt (1988-) (molekuláris biológus) Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos) Novák László (1964-) (idegsebész) Bognár László (1958-) (idegsebész, gyermekidegsebész) Balogh Enikő (1987-) (molekuláris biológus) Paragh György (1953-) (belgyógyász) Kappelmayer János (1960-) (laboratóriumi szakorvos) Bácsi Attila (1967-) (immunológus) Jeney Viktória (1971-) (vegyész, kémia tanár)
Pályázati támogatás:GINOP-2.3.2-15-2016-00005
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5.

001-es BibID:BIBFORM001211
035-os BibID:PMID:17598012
Első szerző:Nagy Béla Jr. (labordiagnosztikai szakorvos)
Cím:Investigation of Thr715Pro P-selectin gene polymorphism and soluble P-selectin levels in type 2 diabetes mellitus / Béla Nagy, Éva Csongrádi, Harjit Pal Bhattoa, István Balogh, György Blaskó, György Paragh, János Kappelmayer, Miklós Káplár
Dátum:2007
Megjegyzések:Increased levels of soluble P-selectin (sP-selectin) have been shown in a number of different disorders, e.g. diabetes mellitus (DM) and cardiovascular disease (CVD). Several studies have attempted to demonstrate the association of the most intensively examined variant of P-selectin gene polymorphism (Thr715Pro) with sP-selectin levels in healthy subjects and in CVD, but contradictory data have been reported.To clarify the effect of Pro715 allele on the sP-selectin levels in type 2 DM, we analysed this polymorphism in diabetic patients and compared these data with sP-selectin levels. Type 2 DM patients (n=119), 48 BMImatched non diabetic individuals - consisting mostly of overweight subjects - and 57 healthy volunteers were included in the study.TheThr715Pro polymorphism was analysed by PCR-RFLP, while sP-selectin levels were measured by ELISA. Significantly elevated sP-selectin levels were found in both DM and in overweight subjects compared to healthy controls. We confirmed previous reports that in healthy Pro715 allele carriers lower sPselectin levels could be measured; however, this difference was only significant in case of lean subjects. No significant difference was detected in sP-selectin level among DM and overweight individuals according to this genotype. However, significant difference was observed in sP-selectin levels in older DM patients compared to younger ones,but these levels were not accounted for by the Thr715Pro polymorphism.We suggest that in type 2 DM individuals, the significantly elevated sP-selectin levels are not due to the Thr715Pro P-selectin gene polymorphism.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Platelet activation
soluble P-selectin
Thr715Pro P-selectin polymorphism
type 2 DM
PADGEM protein
proline
threonine
adult
aged
allele
article
controlled study
DNA polymorphism
enzyme linked immunosorbent assay
female
genetic analysis
genotype
human
human cell
major clinical study
male
non insulin dependent diabetes mellitus
obesity
polymerase chain reaction
priority journal
protein blood level
restriction fragment length polymorphism
thrombocyte activation
Adolescent
Adult
Age Factors
Aged
Amino Acid Substitution
Case-Control Studies
Diabetes Mellitus, Type 2
Female
Humans
Male
Middle Aged
Mutation, Missense
Overweight
P-Selectin
Polymorphism, Genetic
Solubility
egyetemen (Magyarországon) készült közlemény
Megjelenés:Thrombosis and Haemostasis. - 98 (2007), p. 186-191. -
További szerzők:Csongrádi Éva (1969-) (szakorvos) Bhattoa Harjit Pal (1973-) (laboratóriumi szakorvos) Balogh István (1972-) (molekuláris biológus, genetikus) Blaskó György (1947-) (belgyógyász, klinikai farmakológus) Paragh György (1953-) (belgyógyász) Kappelmayer János (1960-) (laboratóriumi szakorvos) Káplár Miklós (1965-) (belgyógyász, diabetológus)
Internet cím:elektronikus változat
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6.

001-es BibID:BIBFORM085378
035-os BibID:(cikkazonosító)8929020
Első szerző:Nyakundi, Benard Bogonko (biokémikus)
Cím:Formation and Detection of Highly Oxidized Hemoglobin Forms in Biological Fluids during Hemolytic Conditions / Nyakundi Benard Bogonko, Erdei Judit, Tóth Andrea, Balogh Enikő, Nagy Andrea, Nagy Béla Jr., Novák László, Bognár László, Paragh György, Kappelmayer János, Jeney Viktória
Dátum:2020
ISSN:1942-0900 1942-0994
Megjegyzések:Hemolytic diseases are characterized by an accelerated breakdown of red blood cells (RBCs) and the release of hemoglobin (Hb). Following, RBC lysis Hb oxidation occurs with the formation of different redox states of Hb (metHb and ferrylHb) and the release of heme. ferrylHb is unstable and decomposes to metHb with the concomitant formation of globin radicals and eventually covalently crosslinked Hb multimers. The goal of the present study was to determine the concentrations of the different redox states of Hb in biological samples during hemolytic conditions. We used plasma and urine samples of mice with intravascular hemolysis and human cerebrospinal fluid (CSF) samples following intraventricular hemorrhage. Because ferrylHb is highly unstable, we also addressed the fate of this species. metHb and free heme time-dependently accumulate in plasma and CSF samples following intravascular hemolysis and intraventricular hemorrhage, respectively. ferrylHb is hardly detectable in the biological samples during hemolytic conditions. Under in vitro conditions, ferrylHb decomposes quickly to metHb, which process is associated with the formation of covalently crosslinked Hb multimers. We detected these covalently crosslinked Hb multimers in plasma, urine, and CSF samples during hemolytic conditions. Because globin modification is specific for these Hb forms, we propose to call this heterogeneous form of Hb produced during ferrylHb decomposition as globin-modified oxidized Hb (gmoxHb). Understanding the formation and the contribution of gmoxHb species to the pathogenesis of hemolytic conditions could have therapeutic implications in the treatment of hemolytic diseases.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Oxidative Medicine and Cellular Longevity. - 2020 (2020), p. 1-13. -
További szerzők:Erdei Judit Zsuzsa (1992-) (vegyész) Tóth Andrea (1992-) (molekuláris biológus) Balogh Enikő (1987-) (molekuláris biológus) Nagy Andrea (1958-) (csecsemő és gyermekgyógyász, neonatológus) Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos) Novák László (1964-) (idegsebész) Bognár László (1958-) (idegsebész, gyermekidegsebész) Paragh György (1953-) (belgyógyász) Kappelmayer János (1960-) (laboratóriumi szakorvos) Jeney Viktória (1971-) (vegyész, kémia tanár)
Pályázati támogatás:GINOP-2.3.2-15-2016-00005
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7.

001-es BibID:BIBFORM078435
035-os BibID:(PMID)30389578
Első szerző:Nyakundi, Benard Bogonko (biokémikus)
Cím:Oxidized hemoglobin forms contribute to NLRP3 inflammasome-driven IL1[beta] production upon intravascular hemolysis / Benard Bogonko Nyakundi, Andrea Tóth, Enikő Balogh, Béla Nagy, Judit Erdei, Bernhard Ryffel, György Paragh, Mario D. Cordero, Viktória Jeney
Dátum:2019
Megjegyzések:Damage associated molecular patterns (DAMPs) are released form red blood cells (RBCs) during intravascular hemolysis (IVH). Extracellular heme, with its pro-oxidant, pro-inflammatory and cytotoxic effects, is sensed by innate immune cells through pattern recognition receptors such as toll-like receptor 4 and nucleotide-binding domain and leucine rich repeat containing family, pyrin domain containing 3 (NLRP3), while free availability of heme is strictly controlled. Here we investigated the involvement of different hemoglobin (Hb) forms in hemolysis-associated inflammatory responses. We found that after IVH most of the extracellular heme molecules are localized in oxidized Hb forms. IVH was associated with caspase-1 activation and formation of mature IL-1β in plasma and in the liver of C57BL/6 mice. We showed that ferrylHb (FHb) induces active IL-1β production in LPS-primed macrophages in vitro and triggered intraperitoneal recruitment of neutrophils and monocytes, caspase-1 activation and active IL-1β formation in the liver of C57BL/6 mice. NLRP3 deficiency provided a survival advantage upon IVH, without influencing the extent of RBC lysis or the accumulation of oxidized Hb forms. However, both hemolysis-induced and FHb-induced pro-inflammatory responses were largely attenuated in Nlrp3-/- mice. Taken together, FHb is a potent trigger of NLRP3 activation and production of IL-1β in vitro and in vivo, suggesting that FHb may contribute to hemolysis-induced inflammation. Identification of RBC-derived DAMPs might allow us to develop new therapeutic approaches for hemolytic diseases.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Ferryl hemoglobin
Heme
Hemolysis
IL-1β
Macrophage
NLRP3 inflammasome activation
Megjelenés:Biochimica et biophysica acta. Molecular basis of disease. - 1865 : 2 (2019), p. 464-475. -
További szerzők:Tóth Andrea (1992-) (molekuláris biológus) Balogh Enikő (1987-) (molekuláris biológus) Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos) Erdei Judit Zsuzsa (1992-) (vegyész) Ryffel, Bernhard Paragh György (1953-) (belgyógyász) Cordero, Mario D. Jeney Viktória (1971-) (vegyész, kémia tanár)
Pályázati támogatás:NKFIH K116024
NKFIH
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